H19 induced by oxidative stress confers temozolomide resistance in human glioma cells via activating NF-&amp;kappa;B signaling

Duan, Shibo; Li, Ming; Wang, Zhifeng; Wang, Longlong; Liu, Yongjie

doi:10.2147/ott.s173244

Cited by 32 publications

(19 citation statements)

References 29 publications

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“…lncRNA H19 is located on chromosome 11 in humans and has been characterized as an oncogenic lncRNA in a few types of cancer due to its promotion of cell proliferation and chemoresistance (15)(16)(17). In the present study, we first found that H19 expression is negatively related to sorafenib sensitivity in HCC cells based on CCK-8 and EdU assays.…”

Section: Discussionmentioning

confidence: 54%

Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675

Liu

et al. 2020

Oncol Rep

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Sorafenib is the first-line treatment for advanced hepatocellular carcinoma (HCC). Since many HCC patients experience drug resistance, there is an urgent need to discover more effective therapeutic strategies to overcome drug resistance. Long non-coding RNAs (lncRNAs) play an important role in tumor drug resistance. However, research on the role of lncRNA H19 in sorafenib resistance in HCC is quite limited. In the present study, CCK-8 assay, RT-qPCR, EdU staining, immunofluorescence staining, and western blot analysis were used to detect the effect of lncRNA H19 on sorafenib resistance of HCC cells. H19 expression was found to be negatively related to sorafenib sensitivity in HCC cells. Knockdown of lncRNA H19 elevated sorafenib sensitivity by suppressing epithelial-mesenchymal transition (EMT) in HCC cells. H19 upregulated miR-675 expression. miR-675 inhibitor decreased the cell viability in sorafenib-treated HCC cells, while miR-675 overexpression had the opposite effect on the treated cells. When the cells were pretreated with miR-675 mimic, H19 siRNA did not alter the effect of miR-675 on sorafenib sensitivity. In conclusion, our study provides new clues for further clinical treatment of sorafenib-resistant liver cancer patients.

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Section: Discussionmentioning

confidence: 54%

Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675

Liu

et al. 2020

Oncol Rep

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“…In the article, it was found that lncRNA growth arrest-specific transcript 5 (GAS5) activated NF-κB signaling pathway and enlarged nerve cell damage via downregulation of miR-124 [ 37 ]. What is more, the regulatory effects of lncRNA H19 on NF-κB signaling pathway have been excavated in various cancers, such as human osteosarcoma [ 38 ], gastric cancer [ 39 ], melanoma [ 40 ], and glioma [ 41 ]. In cancer progression, lncRNA H19 exerted a positive function on the activation of NF-κB signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI)

Qian

Tang

et al. 2021

Translational Neuroscience

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Background Spinal cord injury (SCI) is the most serious complication of spinal injury, often leading to severe dysfunction of the limbs below the injured segment. Conventional therapy approaches are becoming less and less effective, and gene therapy is a new research direction by now. Methods The Sprague-Dawley rats were haphazardly assigned to two groups, namely sham group and SCI model group, and lncRNA H19 and miR-370-3p levels were investigated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Correlation between lncRNA H19 and miR-370-3p was ascertained by luciferase report assay and RT-qPCR. After transfection with si-H19, miR-370-3p inhibitor, negative controls (NC), or both, primary spinal neurons were subjected to the simulation of lipopolysaccharide (LPS) for inducing in vitro model of SCI. Cell viability, apoptotic rate, caspase-3 activity, Bax and Bcl-2 protein, ROS generation, TNF-α, IL-1β, and IL-6 protein, as well as IκBα and p65 phosphorylation ratio were evaluated adopting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), apoptosis, caspase-3 activity, ROS generation, and western blot assays, thereby searching for the specific action mechanism on LPS-induced spinal never injury. Results SCI resulted in lncRNA H19 higher expression and miR-370-3p lower expression. LPS simulation raised a series of cellular biological changes, such as decreased viability, promoted apoptosis, generated ROS, and released inflammatory factors. lncRNA H19 inhibition reversed above LPS-induced changes. Besides, as the downstream target of lncRNA H19, miR-370-3p was oppositely regulated by lncRNA H19. The above biological changes induced by lncRNA H19 inhibition were reversed by miR-370-3p upregulation. Moreover, lncRNA H19 inhibition could block NF-κB pathway through miR-370-3p upregulation. Conclusion Inhibition of lncRNA H19/miR-370-3p mitigated spinal neuron apoptosis in an in vitro model of SCI. This provided the possibility for clinical use of gene therapy.

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“…H19 plays a critical role in the differentiation of many vital organs [25] . H19 may regulate the occurrence and development of tumors, such as breast carcinoma, bladder carcinoma, glioma and colorectal carcinoma [26][27][28] . Significantly, H19 can promote the differentiation of numerous types of cells, such as human mesenchymal stem cells (hMSCs) [29,30] , skeletal muscle satellite cells [31] and myoblasts [32] .…”

Section: Discussionmentioning

confidence: 99%

LncRNA H19 Promotes Odontoblastic Differentiation of Human Dental Pulp Stem Cells by Regulating miR-140-5p and BMP-2/FGF9

Zhong

Kong

et al. 2020

Preprint

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Background: Increasing evidence has revealed that long non-coding RNAs (lncRNAs) exert critical roles in biological mineralization. As a critical process for dentin formation, odontoblastic differentiation is regulated by complex signaling networks. The present study aimed to investigate the biological role and regulatory mechanisms of lncRNA-H19 (H19) in regulating the odontoblastic differentiation of human dental pulp stem cells (hDPSCs). Methods: We performed lncRNA microarray assay to reveal the expression patterns of lncRNAs involved in odontoblastic differentiation. H19 was identified and verified by qRT-PCR. The gain- and loss-of-function studies were performed to investigate the biological role of H19 in regulating odontoblastic differentiation of hDPSCs in vitro and in vivo. Odontoblastic differentiation was evaluated through qRT-PCR, Western blot and Alizarin Red S staining. Bioinformatics analysis identified that H19 could directly interact with miR-140-5p, which was further verified by luciferase reporter assay. After overexpression of miR-140-5p in hDPSCs, odontoblastic differentiation was determined. Moreover, the potential target genes of miR-140-5p were investigated and the biological functions of BMP-2 and FGF9 in hDPSCs were verified. Co-transfection experiments were conducted to validate miR-140-5p was involved in H19-mediated odontoblastic differentiation in hDPSCs.Results: The expression of H19 was significantly up-regulated in hDPSCs undergoing odontoblastic differentiation. Overexpression of H19 stimulated odontoblastic differentiation in vitro and in vivo, whereas down-regulation of H19 revealed the opposite effect. H19 binds directly to miR-140-5p and overexpression of miR-140-5p inhibited odontoblastic differentiation of hDPSCs. H19 acted as a miR-140-5p sponge, resulting in regulated the expression of BMP-2 and FGF9. Overexpression of H19 abrogated the inhibitory effect of miR-140-5p on odontoblastic differentiation.Conclusion: Our data revealed that H19 plays a positive regulatory role in odontoblastic differentiation of hDPSCs through miR-140-5p/BMP-2/FGF9 axis, suggesting that H19 may be a stimulatory regulator of odontogenesis.

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H19 induced by oxidative stress confers temozolomide resistance in human glioma cells via activating NF-κB signaling

Cited by 32 publications

References 29 publications

Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675

Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675

Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI)

LncRNA H19 Promotes Odontoblastic Differentiation of Human Dental Pulp Stem Cells by Regulating miR-140-5p and BMP-2/FGF9

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