H 1 histone and histone variants in Trypanosoma cruzi

Toro, G. Cecilia; Galanti, Norbel

doi:10.1016/0014-4827(88)90137-1

Cited by 43 publications

(35 citation statements)

References 27 publications

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“…In several cases, the original reports stated that HI was absent (van Holde, 1989) and only careful choice of experimental conditions, use of protease inhibitors and specially adapted gel systems allowed the demonstration of its presence. In T. b. brucei, a histone HI-like protein was postulated to be absent (Hecker & Gander, 1985;Hecker et al 1989 et al 1992 a-d), although it was shown in other trypanosomatids (Toro & Galanti, 1988Duschak & Cazzulo, 1990). In the present paper, proteins called e-region were seen in T. b. brucei for the first time.…”

Section: Discussionsupporting

confidence: 45%

“…Additionally, due to their non-extractability with 350 mM NaCl, it could be ruled out that these proteins were non-histones (Johns, 1982). Antibodies directed against HI of sea urchin showed cross-reactivity to the fast migrating proteins of T. cruzi (Toro & Galanti, 1988), the counterparts of the e-bands in T. b. brucei. It has been shown by means of amino acid analysis that these proteins of T. cruzi are histone HI-like proteins (G. C. Toro & N. Galanti, personal communication).…”

Section: Discussionmentioning

confidence: 99%

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Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

et al. 1993

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SUMMARYNucleosome filaments of two stages of the life-cycle of Trypanosoma brucei brucei, namely bloodstream forms and procyclic culture forms, were investigated by electron microscopy. Chromatin of bloodstream forms showed a salt-dependent condensation. The level of condensation was higher than that shown by chromatin from procyclic culture forms, but 30 nm fibres as formed in rat liver chromatin preparations were not found. Analysis of histones provided new evidence for the existence of HI-like proteins, which comigrated in the region of the core histones in SDS-PAGE and in front of the core histones in Triton acid urea gels. Differences were found between the HI-like proteins of the two trypanosome stages as well as between the core histones in their amount, number of bands and banding pattern. It can be concluded that T. b. brucei contains a full set of histones, including HI-like proteins, and that the poor condensation of its chromatin is not due to the absence of H1, but most probably due to histone-DNA interaction being weak. It is obvious that structural and functional differences of the chromatin exist not only between T. b. brucei and higher eukaryotes, but also between various stages of the life-cycle of the parasite. It is therefore not adequate to investigate the chromatin only of the procyclic culture forms as a model for all stages of the life-cycle of T. b. brucei.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

et al. 1993

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“…Chromatin from E. granulosus and F. hepatica was obtained following Stein et al [20] with modifications [3]. Histones were extracted from chromatin, as reported [3,4].…”

Section: Extraction Of Histonesmentioning

confidence: 99%

“…Histones were analyzed by Triton DF16-acid-urea polyacrylamide gel electrophoresis (TAU-PAGE), as described [3,4].…”

Section: Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

“…However, these chromosomal proteins present a high degree of divergence in lower eukaryotes, such as Entamoeba [1], Trichomonas [2], Trypanosoma [3][4][5][6][7][8], Leishmania and Crithidia [9], dinoflagellates [10], ciliata [11] and Giardia [12]. Histone divergence during evolution is expressed at the amino acid sequence level in core histones and mostly at the structural level in H1 histones.…”

Section: Introductionmentioning

confidence: 99%

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Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones

et al. 2004

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Histones from the parasitic platyhelminthes, Echinococcus granulosus and Fasciola hepatica, were systematically characterized. Core histones H2A, H2B, H3 and H4, which were identified on the basis of amino acid sequencing and mass spectrometry data, showed conserved electrophoretic patterns. Histones H1, identified on the basis of physicochemical properties, amino acid composition and amino acid sequencing, showed divergence, both in their number and electrophoretic mobilities, between the two species and among other organisms. According to these data, core histones but not H1 histones, would be stabilized during evolution at the level of platyhelminthes.

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Presence of an unusually high concentration of an ubiquitinated histone-like protein inTrypanosoma cruzi

1997

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The conjugation of ubiquitin to histones H2A and H2B has been established in higher eukaryotes and has been related to changes in chromatin organization. In Trypanosoma cruzi, no condensation of chromatin occurs during mitosis. In order to determine the presence of histone ubiquitination in T. cruzi epimastigotes, histones were extracted from chromatin and analyzed by three electrophoretic systems: acid-urea, triton-acid-urea and sodium-dodecyl-sulphate polyacrylamide gel. The immunochemical detection of ubiquitin-histone conjugates by Western blotting showed a strong reaction with a slow migrating band of M(r) 19 kDa. The high percentage of ubiquitin-histone conjugates present in T. cruzi chromatin may be related to the inability of this parasite to condense chromatin into a 30 nm fiber.

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H 1 histone and histone variants in Trypanosoma cruzi

Cited by 43 publications

References 27 publications

Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones

Presence of an unusually high concentration of an ubiquitinated histone-like protein inTrypanosoma cruzi

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