Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling

Ye, Qifa; Zhu, Yi; Ye, Shaojun; Liu, Hong; She, Xiaojun; Niu, Ying; Ming, Yingzi

doi:10.3892/etm.2016.3034

Cited by 23 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An earlier study demonstrated that Gyp treatment protected rat cortical cells from glutamate-induced apoptosis by increasing the level of anti-apoptotic Bcl-2 and inhibiting pro-apoptotic Bax (Shang et al, 2006). Recent studies showed that Gyp suppressed apoptosis in cardiomycocytes in a myocardial ischemia-reperfusion injury rat model (Yu et al, 2016c) and inhibited renal cell apoptosis in a renal ischemia-reperfusion injury mouse model (Ye et al, 2016), both through upregulation of Bcl-2 and dowregulation of Bax. Gyp treatment also inhibited hepatic cell apoptosis in a mouse hepatic ischemia-reperfusion injury model by increasing Bcl-2 expression and decreasing protein levels of Bax and cytochrome c, and the activities of Caspase 3/8 (Zhao et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Yu et al (2016a) reported that Gyp treatment suppressed NF-kB activation by inhibition of MAPK pathway in both oxygen-glucose deprivation-reoxygenation H9c2 cell model and ischemia-reperfusion injury rat model (Yu et al, 2016a). Gyp exposure has also been shown to result in decreased production of pro-inflammatory cytokines in a renal ischemia/reperfusion injury mouse model and in β amyloidincubated N9 microglia cells (Cai et al, 2016;Ye et al, 2016). Interestingly, a recent study demonstrated that Gyp treatment attenuated inflammation in the retina and optic nerve in a mouse model of experimental autoimmune optic neuritis (Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gypenosides protect retinal pigment epithelium cells from oxidative stress

Alhasani

Biswas

Tohari

et al. 2018

Food and Chemical Toxicology

View full text Add to dashboard Cite

Oxidative stress plays a critical role in the pathogenesis of retinal degeneration. Gypenosides are the major functional components isolated from Gynostemma pentaphyllum. They have been shown to protect against oxidative stress and inflammation and have also demonstrated a protective effect on experimental optic neuritis. In order to determine the protective properties of gypenosides against oxidative stress in human retinal pigment epithelium (RPE) cells, ARPE-19 cells were treated with HO or HO plus gypenosides for 24 h. ARPE-19 cells co-treated with gypenosides had significantly increased cell viability and decreased cell death rate when compared to cells treated with HO alone. The level of GSH, the activities of SOD and catalase, and the expression of NRF2 and antioxidant genes were notably decreased, while there were marked increases in ROS, MDA and pro-inflammatory cytokines in ARPE-19 cells exposed to HO; co-treatment with gypenosides significantly counteract these changes. Our study suggests that gypenosides protect RPE cells from oxidative damage and offer therapeutic potential for the treatment of retinal degeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gypenosides protect retinal pigment epithelium cells from oxidative stress

Alhasani

Biswas

Tohari

et al. 2018

Food and Chemical Toxicology

View full text Add to dashboard Cite

show abstract

“…Geniposide also has been revealed to have anti-inflammatory activities by blocking the downregulation of toll-like receptor 4 (TLR4) expression that is upregulated by lipopolysaccharide (LPS) [ 18 ]. The pharmacological effects of geniposide also exhibited inhibition of histamine when released on atopic dermatitis and protective effects against ischemia and reperfusion-induced renal injury [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Preclinical Pharmacokinetics of Scoparone, Geniposide and Rhein in an Herbal Medicine Using a Validated LC-MS/MS Method

Hsueh

Tsai

2018

Molecules

View full text Add to dashboard Cite

The herbal formula Yin-Chen-Hao-Tang has been reported to have anti-fibrosis properties. The aim of this study was to reveal the pharmacokinetic characteristics of bioactive compounds in this herbal formula. A new high-performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of scoparone, geniposide and rhein in rat plasma. A pharmaceutical herbal powder was administered to rats at doses of 1 g/kg and 3 g/kg orally. The method showed excellent linearity (r2 > 0.999) and validation was successfully conducted for the pharmacokinetic study. The results show that the Cmax values and areas under the curve of scoparone, geniposide and rhein were higher and not proportional to the dose in rat plasma, while the Tmax and half-life values were consistent in the group that received 1 g/kg. The clearance of the higher dose (3 g/kg) did not decrease proportionally to that of the low dose. The results showed the nonlinear pharmacokinetic properties of scoparone, geniposide and rhein in Yin-Chen-Hao-Tang that suggested possible accumulation of bioactive compounds through oral administration. This pharmacokinetic study reveals that an increased dose of this herbal formula would largely increase the maximum concentration and bioavailability of scoparone, geniposide and rhein.

show abstract

“…Gypenosides are the main chemical and pharmacological substances of the Chinese herb gynostemma. Previous clinical trials have revealed that gypenosides can significantly attenuate the levels of BUN and CRE and reduces oxidative stress [ 8 ]. Chitosan, a new type of biomedical raw material, has attracted much attention in China and abroad.…”

Section: Introductionmentioning

confidence: 99%

Combination therapy of chitosan, gynostemma, and motherwort alleviates the progression of experimental rat chronic renal failure by inhibiting STAT1 activation

Bai

Wang

et al. 2018

Oncotarget

View full text Add to dashboard Cite

This study aimed to investigate the effect of single and combination therapy using chitosan (K), gynostemma (J), and motherwort (Y) on an experimental rat model of chronic renal failure (CRF) induced by adenine and the underlying mechanisms. CRF rats were treated with individual or combinational therapy with two or three of these agents. Biochemical indicators showed that the levels of blood urea nitrogen, creatinine and uric acid decreased and the levels of albumin and hemoglobin increased by single or combination therapy of these drugs. Drug treatment also decreased oxidative stress damage of renal tissues in CRF rats. Histopathological lesions were attenuated in each drug treatment group by various degrees. Additionally, drug treatment affected the expression of extracellular matrix (ECM) proteins including plasminogen activator inhibitor 1, collagen I, matrix metalloprotease-1, and tissue inhibitor of metalloproteinases 1. In particular, the combination therapy of K, J, and Y was superior to the respective monotherapy, which supported the prescription of KJY combination. We further studied the inhibitory effect of KJY on LPS-induced inflammation in RAW264.7 macrophages. The results showed that KJY inhibited LPS-induced secretion of inflammatory cytokines (Interferon-gamma, Interleukin-1 Beta, chemokine (C-X-C motif) ligand 10, cyclooxygenase-2 and Tumor necrosis factor-α in RAW264.7 macrophages. Combination therapy of KJY suppressed the protein expression of Cyclooxygenase-2 and inducible nitric oxide synthase in vivo and in vitro. Further study indicated that KJY inhibited STAT1 activation by down regulating p-STAT1 to exert anti-inflammatory effect and improve renal function in rats with chronic renal failure.

show abstract

Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling

Cited by 23 publications

References 32 publications

Gypenosides protect retinal pigment epithelium cells from oxidative stress

Gypenosides protect retinal pigment epithelium cells from oxidative stress

Preclinical Pharmacokinetics of Scoparone, Geniposide and Rhein in an Herbal Medicine Using a Validated LC-MS/MS Method

Combination therapy of chitosan, gynostemma, and motherwort alleviates the progression of experimental rat chronic renal failure by inhibiting STAT1 activation

Contact Info

Product

Resources

About