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Abstract

BackgroundPolymorphisms in the gene that encodes the human cellular receptor for group B coxsackieviruses and adenoviruses (HCAR) could be responsible for differences in susceptibility to infections with these pathogens. Moreover, adenovirus subgroup C-mediated gene therapy could be influenced by mutations in the coding exons for the aminoterminal immunoglobulin-like 1 (Ig1) domain, which is the essential component for adenovirus fiber knob binding.ResultsUsing two primersets in the adjacent intron sequences, HCAR exons 2 and 3, which comprise the full-length Ig1 domain, were amplified by polymerase chain reactions in 108 unselected and unrelated healthy Belgian volunteers. After nucleotide sequencing, no polymorphisms could be demonstrated in the adenovirus-binding Ig1 exons 2 and 3 of the HCAR gene.ConclusionsThe adenovirus-binding Ig1 domain seems to be a highly conserved region in the Caucasian population which is a reassuring finding regarding adenovector-based gene therapy.

Genetic variation and population structure in China summer maize germplasm

“…Wu et al 15 shows that 700–1000 SNPs were necessary to robustly estimate the genetic difference among subpopulations, our result shows that about 5000 tagSNPs and haplotype loci derived from them are suitable for population structure analysis and germplasm study. In maize and many other organisms, it has also been reported that haplotype loci derived from tagSNPs are more informative than binary SNPs 16 , 40 , 41 .…”

PPARγ2Pro12Ala Polymorphism and Human Health

“…However, further studies in various ethnic populations demonstrate that effect of this mutation on body mass is more complex. An association of Ala12 variant with decreased adiposity is confirmed in diabetic, nondiabetic, or healthy subjects [ 53 – 58 ]. In studies involving an African American population and a white American population, this mutation is associated with lower BMI in African Americans and increased BMI in white Americans [ 59 , 60 ], indicating that same genetic mutation results in different responses in different ethnic groups.…”

Mining Grapevine Downy Mildew Susceptibility Genes: A Resource for Genomics-Based Breeding and Tailored Gene Editing

“…Since the first whole plant genome to be sequenced [ 27 ], de novo and reference-based SNP discovery and application are now feasible for numerous plant species. Large-scale SNP discovery was performed in almost all sequenced plant genomes such as maize [ 28 ], Arabidopsis [ 29 ], rice [ 30 ], rapeseed [ 31 ], potato [ 32 ], and pepper [ 33 ]. On the method side, Genotyping-By-Sequencing (GBS) has recently emerged as a promising genomic approach to explore plant genetic diversity on a genome-wide scale [ 34 ], followed by the more cost-effective Genotyping-in-Thousands by sequencing (GT-seq) [ 35 ].…”