GWAS for Stripe Rust Resistance in Wild Emmer Wheat (Triticum dicoccoides) Population: Obstacles and Solutions

Tene, May; Adhikari, Elina; Cobo, Nicolás; Jordan, Katherine; Matny, Oadi; Blanco, Isabel A. del; Roter, Jonathan; Ezrati, Smadar; Govta, Liubov; Manisterski, J.; Yehuda, Pnina Ben; Chen, Xianming; Steffenson, Brian J.; Akhunov, Eduard; Sela, Hanan

doi:10.3390/crops2010005

Cited by 7 publications

(3 citation statements)

References 91 publications

(105 reference statements)

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“…Our diversity panel may harbor other race-specific rust resistance genes against other races not tested herein. Testing of this diversity panel with other rust races may reveal other resistance genes/QTLs as reported for the Ethiopian Durum Wheat panel tested with four Pst races that possess diverse virulence profiles ( Tene et al., 2022 ).…”

Section: Discussionmentioning

confidence: 87%

“…There was no overlap between any of the QTN loci, which was expected because all multi-disease resistance genes known to date are APR genes ( Chen and Kang, 2017 ) and these could not be detected in our seedling tests. Discovery of APR genes could be achieved by evaluating severity in field experiments ( Fatima et al., 2020 ; Tene et al., 2022 ), which is a proposed future direction for exploring the potential and exploiting the value of this diversity panel. Expansion to include traits other than disease resistance, such as morphological traits, is also anticipated to lead to interesting and beneficial outcomes with possible application in wheat breeding programs.…”

Section: Discussionmentioning

confidence: 99%

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Genetic architecture of rust resistance in a wheat (Triticum turgidum) diversity panel

Klymiuk

Haile²,

Ens³

et al. 2023

Front. Plant Sci.

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IntroductionWheat rust diseases are widespread and affect all wheat growing areas around the globe. Breeding strategies focus on incorporating genetic disease resistance. However, pathogens can quickly evolve and overcome the resistance genes deployed in commercial cultivars, creating a constant need for identifying new sources of resistance.MethodsWe have assembled a diverse tetraploid wheat panel comprised of 447 accessions of three Triticum turgidum subspecies and performed a genome-wide association study (GWAS) for resistance to wheat stem, stripe, and leaf rusts. The panel was genotyped with the 90K Wheat iSelect single nucleotide polymorphism (SNP) array and subsequent filtering resulted in a set of 6,410 non-redundant SNP markers with known physical positions.ResultsPopulation structure and phylogenetic analyses revealed that the diversity panel could be divided into three subpopulations based on phylogenetic/geographic relatedness. Marker-trait associations (MTAs) were detected for two stem rust, two stripe rust and one leaf rust resistance loci. Of them, three MTAs coincide with the known rust resistance genes Sr13, Yr15 and Yr67, while the other two may harbor undescribed resistance genes.DiscussionThe tetraploid wheat diversity panel, developed and characterized herein, captures wide geographic origins, genetic diversity, and evolutionary history since domestication making it a useful community resource for mapping of other agronomically important traits and for conducting evolutionary studies.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Genetic architecture of rust resistance in a wheat (Triticum turgidum) diversity panel

Klymiuk

Haile²,

Ens³

et al. 2023

Front. Plant Sci.

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“…A Mexican core set of wheat accessions representing the complete variation of hexaploid landraces was found to include novel APR to both yellow rust and stem rust [ 51 ]. In addition to landraces, crop wild relatives, such as wild emmer wheat ( T. turgidum ), are also reported to carry novel sources of APR to stripe rust [ 52 ].…”

Section: Apr: the Wheat-rust Pathosystemmentioning

confidence: 99%

Harnessing adult-plant resistance genes to deploy durable disease resistance in crops

Dinglasan

Periyannan

Hickey

2022

Essays in Biochemistry

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Adult-plant resistance (APR) is a type of genetic resistance in cereals that is effective during the later growth stages and can protect plants from a range of disease-causing pathogens. Our understanding of the functions of APR-associated genes stems from the well-studied wheat-rust pathosystem. Genes conferring APR can offer pathogen-specific resistance or multi-pathogen resistance, whereby resistance is activated following a molecular recognition event. The breeding community prefers APR to other types of resistance because it offers broad-spectrum protection that has proven to be more durable. In practice, however, deployment of new cultivars incorporating APR is challenging because there is a lack of well-characterised APRs in elite germplasm and multiple loci must be combined to achieve high levels of resistance. Genebanks provide an excellent source of genetic diversity that can be used to diversify resistance factors, but introgression of novel alleles into elite germplasm is a lengthy and challenging process. To overcome this bottleneck, new tools in breeding for resistance must be integrated to fast-track the discovery, introgression and pyramiding of APR genes. This review highlights recent advances in understanding the functions of APR genes in the well-studied wheat-rust pathosystem, the opportunities to adopt APR genes in other crops and the technology that can speed up the utilisation of new sources of APR in genebank accessions.

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A new wild emmer wheat panel allows to map new loci associated with resistance to stem rust at seedling stage

Mastrangelo,

Roncallo,

Matny

et al. 2023

The Plant Genome

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Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a major wheat disease worldwide. A collection of 283 wild emmer wheat [Triticum turgidum L. subsp. dicoccoides (Körn. ex Asch. & Graebn.) Thell] accessions, representative of the entire Fertile Crescent region where wild emmer naturally occurs, was assembled, genotyped, and characterized for population structure, genetic diversity, and rate of linkage disequilibrium (LD) decay. Then, the collection was employed for mapping Pgt resistance genes, as a proof of concept of the effectiveness of genome‐wide association studies in wild emmer. The collection was evaluated in controlled conditions for reaction to six common Pgt pathotypes (TPMKC, TTTTF, JRCQC, TRTTF, TTKSK/Ug99, and TKTTF). Most resistant accessions originated from the Southern Levant wild emmer lineage, with some showing a resistance reaction toward three to six tested races. Association analysis was conducted considering a 12K polymorphic single‐nucleotide polymorphisms dataset, kinship relatedness between accessions, and population structure. Eleven significant marker–trait associations (MTA) were identified across the genome, which explained from 17% to up to 49% of phenotypic variance with an average 1.5 additive effect (based on the 1–9 scoring scale). The identified loci were either effective against single or multiple races. Some MTAs colocalized with known Pgt resistance genes, while others represent novel resistance loci useful for durum and bread wheat prebreeding. Candidate genes with an annotated function related to plant response to pathogens were identified at the regions linked to the resistance and defined according to the estimated small LD (about 126 kb), as typical of wild species.

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GWAS for Stripe Rust Resistance in Wild Emmer Wheat (Triticum dicoccoides) Population: Obstacles and Solutions

Cited by 7 publications

References 91 publications

Genetic architecture of rust resistance in a wheat (Triticum turgidum) diversity panel

Genetic architecture of rust resistance in a wheat (Triticum turgidum) diversity panel

Harnessing adult-plant resistance genes to deploy durable disease resistance in crops

A new wild emmer wheat panel allows to map new loci associated with resistance to stem rust at seedling stage

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