Untitled

Kuznetsova, Marina; Lee, Cindy

doi:10.1007/s00027-002-8070-0

Cited by 79 publications

(36 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tyr showed smaller EFSML (2.1-18.7) caused by significantly smaller concentration in ULW and SML samples (Table S3). Our study confirmed previous observations that the SML is often non-uniformly enriched with FAA (Kuznetsova and Lee, 2002;Reinthaler et al, 2008;van Pinxteren et al, 2012;Engel and Galgani, 2016). Different factors, such as the transport of FAA from the ULW to the SML, an in situ production via extracellular hydrolysis of CAA or a direct release of FAA by cell lysis can cause the observed enrichment of FAA in the SML.…”

Section: Seawater Samplessupporting

confidence: 92%

“…were reported in different oceanic areas in previous studies (Kuznetsova and Lee, 2002;Kuznetsova et al, 2004;Reinthaler et al, 2008;van Pinxteren et al, 2012;Engel and Galgani, 2016). Reinthaler et al (2008) reported concentrations of dissolved https://doi.org/10.5194/acp-2019-976 Preprint.…”

Section: Seawater Samplessupporting

confidence: 62%

“…Moreover, Kuznetsova and Lee (2002) suggested that the intracellular pools of organisms, which are rich in DFAA and DCAA compared to seawater, can be leaching out by stressed microorganisms resulting in an release of DFAA and DFAA with an effect on DFAA and DCAA pools in seawater. On the basis of previous studies, the transportation and the releasing mechanisms seems to be most likely for the observed enrichment of FAA.…”

Section: Seawater Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Concerted measurements of free amino acids at the Cape Verde Islands: High enrichments in submicron sea spray aerosol particles and cloud droplets

Triesch¹,

Pinxteren²,

Engel³

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract. This study presents measurements of free amino acids (FAA) in the marine environment to elucidate their transfer from the ocean into the atmosphere to marine aerosol particles and to clouds. FAA were investigated in seawater (underline water (ULW), sea surface microlayer (SML)), in ambient marine size-segregated aerosol particle samples at two heights (ground based at the Cape Verde Atmospheric Observatory (CVAO) and at the Mt. Verde, 744 m height) and in cloud water using concerted measurements. The ∑FAA concentration in the SML varied between 0.13–3.64 µmol L−1, in the ULW between 0.01–1.10 µmol L−1 and a strong enrichment of ∑FAA in the SML (EFSML: 1.1–298.4, average of 57.2) was observed. In the submicron (0.05–1.2 µm) aerosol particles at the CVAO, the composition of FAA was more complex and higher atmospheric concentration of ∑FAA (up to 6.3 ng m−3) compared to the supermicron (1.2–10 µm) aerosol particles (maxima of 0.5 ng m−3) were observed. The total ∑FAA concentration (PM10) was between 1.8–6.8 ng m−3 and tended to increase during the campaign. Averaged ∑FAA concentrations on the aerosol particles at the Mt. Verde were lower (submicron: 1.5 ng m−3, supermicron: 1.2 ng m−3) compared to the CVAO. A similar percentage contribution of ∑FAA to dissolved organic carbon (DOC) in the seawater (up to 7.6&tinsp;%) and to water-soluble organic carbon (WSOC) on the submicron aerosol particles (up to 5.3 %) indicated a related transfer process of FAA and DOC in the marine environment. The FAA were strongly enriched in the submicron aerosol particles (EFaer(∑FAA) 4∙102–3∙104, EFaer(WSOC) 2∙103–1∙104), possibly resulting from film droplet formation. The enrichment in supermicron aerosol particles was several orders of magnitude lower compared to supermicron size range with EFaer(∑FAA) 1∙101–2∙101, EFaer(WSOC) 3∙102–4∙102. A case study showed that several amino acids were transported from the ocean up to cloud level (e.g. aspartic acid, glutamic acid, proline) while other amino acids might not be transferred or quickly degraded (e.g. phenylalanine, tyrosine) or produced (e.g. GABA). The cloud water samples exhibited a similar composition of FAA compared to the SML but a strong variation of the atmospheric concentration of ∑FAA during the campaign (11.2–489.9 ng m−3). FAA in cloud water samples showed a strong enrichment by a factor of 4∙103 compared to the SML. The presence of high concentrations of FAA in general and of biologically produced FAA (aspartic acid) in particular together with the presence of inorganic marine tracers (sodium, methane sulfonic acid) demonstrates the influence of oceanic sources on marine clouds.

show abstract

Section: Seawater Samplessupporting

confidence: 92%

Section: Seawater Samplessupporting

confidence: 62%

Section: Seawater Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Concerted measurements of free amino acids at the Cape Verde Islands: High enrichments in submicron sea spray aerosol particles and cloud droplets

Triesch¹,

Pinxteren²,

Engel³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The unique development of certain alphaproteobacterial and gammaproteobacterial genera may also explain the much lower production of AVFA fragments during the bottom water incubation compared to the surface water incubation ( Figures 2B,D ). Either these bacteria directly took up the peptide, or the hydrolysis and subsequent uptake of the fragments were tightly coupled (Fuhrman, 1987; Kuznetsova and Lee, 2002; Liu et al, 2013). These two processes cannot be differentiated with these data, but regardless, both pathways differ from that of the surface incubation, where hydrolysis and uptake seem uncoupled.…”

Section: Discussionmentioning

confidence: 99%

“…DFAA were calculated as sum of individual amino acids analyzed in high performance liquid chromatography (HPLC, Shimadzu Prominence) equipped with a fluorescence detector after pre-column o -phthaldialdehyde (OPA) derivatization (Lindroth and Mopper, 1979; Lee et al, 2000). TDAA were analyzed in the same way as DFAA but after hydrolysis in 6 N HCl under nitrogen at 110°C for 20 h (Kuznetsova and Lee, 2002). DCAA were calculated as TDAA subtracting DFAA.…”

Section: Methodsmentioning

confidence: 99%

Different Bacterial Communities Involved in Peptide Decomposition between Normoxic and Hypoxic Coastal Waters

2017

View full text Add to dashboard Cite

Proteins and peptides are key components of the labile dissolved organic matter pool in marine environments. Knowing which types of bacteria metabolize peptides can inform the factors that govern peptide decomposition and further carbon and nitrogen remineralization in marine environments. A 13C-labeled tetrapeptide, alanine-valine-phenylalanine-alanine (AVFA), was added to both surface (normoxic) and bottom (hypoxic) seawater from a coastal station in the northern Gulf of Mexico for a 2-day incubation experiment, and bacteria that incorporated the peptide were identified using DNA stable isotope probing (SIP). The decomposition rate of AVFA in the bottom hypoxic seawater (0.018–0.035 μM h-1) was twice as fast as that in the surface normoxic seawater (0.011–0.017 μM h-1). SIP experiments indicated that incorporation of 13C was highest among the Flavobacteria, Sphingobacteria, Alphaproteobacteria, Acidimicrobiia, Verrucomicrobiae, Cyanobacteria, and Actinobacteria in surface waters. In contrast, highest 13C-enrichment was mainly observed in several Alphaproteobacteria (Thalassococcus, Rhodobacteraceae, Ruegeria) and Gammaproteobacteria genera (Colwellia, Balneatrix, Thalassomonas) in the bottom water. These data suggest that a more diverse group of both oligotrophic and copiotrophic bacteria may be involved in metabolizing labile organic matter such as peptides in normoxic coastal waters, and several copiotrophic genera belonging to Alphaproteobacteria and Gammaproteobacteria and known to be widely distributed may contribute to faster peptide decomposition in the hypoxic waters.

show abstract

Crustacean zooplankton release copious amounts of dissolved organic matter as taurine in the ocean

Clifford

Hansell

Varela

et al. 2017

Limnology & Oceanography

View full text Add to dashboard Cite

Taurine (Tau), an amino acid‐like compound, is present in almost all marine metazoans including crustacean zooplankton. It plays an important physiological role in these organisms and is released into the ambient water throughout their life cycle. However, limited information is available on the release rates by marine organisms, the concentrations and turnover of Tau in the ocean. We determined dissolved free Tau concentrations throughout the water column and its release by abundant crustacean mesozooplankton at two open ocean sites (Gulf of Alaska and North Atlantic). At both locations, the concentrations of dissolved free Tau were in the low nM range (up to 15.7 nM) in epipelagic waters, declining sharply in the mesopelagic to about 0.2 nM and remaining fairly stable throughout the bathypelagic waters. Pacific amphipod–copepod assemblages exhibited lower dissolved free Tau release rates per unit biomass (0.8 ± 0.4 μmol g−1 C‐biomass h−1) than Atlantic copepods (ranging between 1.3 ± 0.4 μmol g−1 C‐biomass h−1 and 9.5 ± 2.1 μmol g−1 C‐biomass h−1), in agreement with the well‐documented inverse relationship between biomass‐normalized excretion rates and body size. Our results indicate that crustacean zooplankton might contribute significantly to the dissolved organic matter flux in marine ecosystems via dissolved free Tau release. Based on the release rates and assuming steady state dissolved free Tau concentrations, turnover times of dissolved free Tau range from 0.05 d to 2.3 d in the upper water column and are therefore similar to those of dissolved free amino acids. This rapid turnover indicates that dissolved free Tau is efficiently consumed in oceanic waters, most likely by heterotrophic bacteria.

show abstract

Untitled

Cited by 79 publications

References 52 publications

Concerted measurements of free amino acids at the Cape Verde Islands: High enrichments in submicron sea spray aerosol particles and cloud droplets

Concerted measurements of free amino acids at the Cape Verde Islands: High enrichments in submicron sea spray aerosol particles and cloud droplets

Different Bacterial Communities Involved in Peptide Decomposition between Normoxic and Hypoxic Coastal Waters

Crustacean zooplankton release copious amounts of dissolved organic matter as taurine in the ocean

Contact Info

Product

Resources

About