Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host

Heekin, Andrew M.; Guerrero, Felix D.; Bendele, Kylie G.; Saldivar, Leo; Scoles, Glen A.; Dowd, Scot E.; Gondro, Cedric; Nene, Vishvanath; Djikeng, Appolinaire; Brayton, Kelly A.

doi:10.1007/s00436-013-3482-4

Cited by 37 publications

(27 citation statements)

References 58 publications

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“…Similar results were observed in the gut transcriptome of D. variabilis, for which the percentage of secreted transcripts among the genes expressed was small (ANDERSON et al, 2008). Fifty-three percentage of the transcripts differentially expressed in the gut transcriptome of R. microplus females were classified as intracellular and 18% as membranous (HEEKIN et al, 2013).…”

Section: Discussionsupporting

confidence: 69%

“…The classification from the automated analysis showed that the category of catalytic activity category was prevalent, thus indicating that many of the digestive tract transcripts were enzymes, such as oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. In the gut transcriptome of R. microplus, binding and catalytic activity was also identified as the most common functional types of activity (HEEKIN et al, 2013). The same results were observed by Xu et al (2016), who classified 47 and 42% of the transcripts of H. flava in the functional categories binding and catalytic activity, respectively.…”

Section: Discussionsupporting

confidence: 67%

“…Thus, knowledge about the vital molecular constituents of ticks, such as genes and/or proteins, have become the target of several studies (CASTRO et al, 2016;CHMELAŘ et al, 2016;DÍAZ-MARTÍN et al, 2015;HEEKIN et al, 2013;KOTSYFAKIS et al, 2015;PERNER et al, 2016;RIBEIRO et al, 2017;XU et al, 2016). These studies have sought to ascertain and understand the roles of the molecules involved in the tick-host or tick-pathogen relationship.…”

Section: Introductionmentioning

confidence: 99%

“…Peptidases such as cathepsins, legumain, aspartic, metallo and serine proteinase are cited as the main proteolytic enzymes actives in the digestive machinery of hemoglobin (SOJKA et al, 2013). All these peptidases have been identified and characterized by biochemical, proteomic and transcriptomic studies in different tick species, such as Dermacentor variabilis (Say, 1821) (ANDERSON et al, 2008), Rhipicephalus microplus (Canestrini, 1888) (MENDIOLA et al, 1996;KONGSUWAN et al, 2010;HEEKIN et al, 2013), Ixodes ricinus Linneaus, 1758 (SOJKA et al, 2007(SOJKA et al, , 2008(SOJKA et al, , 2013HORN et al, 2009;FRANTA et al, 2010;KOTSYFAKIS et al, 2015;PERNER et al, 2016), Haemaphysalis longicornis Neumann, 1901(KOH et al, 1990BOLDBAATAR et al, 2006), Haemaphysalis flava Neumann 1897 (XU et al, 2016), Ornithodoros tholozani (Laboulbène and Mégnin, 1882) (AKOV et al, 1976), Ornithodoros moubata (Murray, 1877) (GRANDJEAN, 1984) and Ornithodoros erraticus (Lucas, 1849) (OLEAGA et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The gut transcriptome of the species D. variabilis has been explored, thus resulting in identification of 82 transcripts directly involved in the blood meal digestion process (ANDERSON et al, 2008). A gut transcriptome study on females of R. microplus, in which the expression profiles of some genes of infected and non-infected specimens with those of the protozoon Babesia bovis were compared, showed thirty-three genes with higher levels of expression in gut samples from female ticks feeding on a B. bovis-infected calf (HEEKIN et al, 2013). Schwarz et al (2014) studied the transcriptome and proteome of the midgut and salivary gland of I. ricinus and identified 217 and 110 genes expressed exclusively in the midgut and in the salivary gland, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to know and ascertain the main molecules expressed in the gut of argasid after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon using 454-based RNA sequencing. The gut transcriptome analysis reveals several transcripts associated with hemoglobin digestion, such as serine, cysteine, aspartic proteases and metalloenzymes. The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. We recorded the presence a cathepsin O peptidase-coding transcript in ticks. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on mitochondrial genome and nuclear rRNA sequences. We deposited 2,213 sequence of O. mimon to the public databases. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity.Keywords: Argasidae, transcriptome, gut, phylogeny, Ornithodoros mimon. ResumoOrnithodoros mimon é um carrapato argasídeo parasita de morcegos, aves e marsupiais, além de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de moléculas vitais no processo de digestão e na relação parasito-hospedeiro, além de fornecer também informações sobre a evolução dos artrópodes hematófagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais moléculas expressas no intestino de uma espécie de carrapato argasídeo após o repasto sanguíneo, através de uma análise transcritômica descritiva do intestino de fêmeas ingurgitadas de O. mimon, utilizando um sequenciamento de RNA de nova geração da plataforma 454. Além de inferir a relação filogenética de carrapatos através de um conjunto de dados transcritômicos. O transcriptoma do intestino revelou diversos transcritos associados com a digestão da hemoglobina, como proteinases das classes serino, cisteína, aspártica e metalo. Registramos a presença de um transcrito de uma cisteína peptidase do tipo catepsina O em carrapatos. A inferência filogenética baseada em conjunto de dados transcritos homólogos tem uma resolução topológica similar a de outros conjuntos de dados moleculares. Foram depositados no banco de dados gênico público 2213 transcritos de O. mimon. Os achados obtidos no presente estudo podem contribuir para compreensão dos importantes processos, como digestão, nutrição e imunidade dos carrapatos da família Argasidae, além de fornecer informações sobre a filogenia da ordem Ixodida.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Transmission-Blocking Vaccines: Focus on Anti-Vector Vaccines against Tick-Borne Diseases

Neelakanta

Sultana

2014

Arch. Immunol. Ther. Exp.

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Tick-borne diseases are a potential threat that account for significant morbidity and mortality in human population worldwide. Vaccines are not available to treat several of the tick-borne diseases. With the emergence and resurgence of several tick-borne diseases, emphasis on the development of transmission-blocking vaccines remains increasing. In this review, we provide a snap shot on some of the potential candidates for the development of anti-vector vaccines (a form of transmission-blocking vaccines) against wide range of hard and soft ticks that include Ixodes, Haemaphysalis, Dermacentor, Amblyomma, Rhipicephalus and Ornithodoros species.

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Constructing and detecting a cDNA library for mites

Zhao

Juan

et al. 2015

Parasitol Res

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RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is to explore a better method to construct cDNA library for mites that will lay the foundation on transcriptome and molecular pathogenesis research. We selected Psoroptes cuniculi as an experimental subject and took the following steps to construct and verify cDNA library. First, we combined liquid nitrogen grinding with TRIzol for total RNA extraction. Then, switching mechanism at 5' end of the RNA transcript (SMART) technique was used to construct full-length cDNA library. To evaluate the quality of cDNA library, the library titer and recombination rate were calculated. The reliability of cDNA library was detected by sequencing and analyzing positive clones and genes amplified by specific primers. The results showed that the RNA concentration was 836 ng/μl and the absorbance ratio at 260/280 nm was 1.82. The library titer was 5.31 × 10(5) plaque-forming unit (PFU)/ml and the recombination rate was 98.21%, indicating that the library was of good quality. In the 33 expressed sequence tags (ESTs) of P. cuniculi, two clones of 1656 and 1658 bp were almost identical with only three variable sites detected, which had an identity of 99.63% with that of Psoroptes ovis, indicating that the cDNA library was reliable. Further detection by specific primers demonstrated that the 553-bp Pso c II gene sequences of P. cuniculi had an identity of 98.56% with those of P. ovis, confirming that the cDNA library was not only reliable but also feasible.

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Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host

Cited by 37 publications

References 58 publications

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Transmission-Blocking Vaccines: Focus on Anti-Vector Vaccines against Tick-Borne Diseases

Constructing and detecting a cDNA library for mites

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