Gut REG3γ-Associated Lactobacillus Induces Anti-inflammatory Macrophages to Maintain Adipose Tissue Homeostasis

Huang, Yugang; Qi, Houbao; Zhang, Zhiqian; Wang, Enlin; Yun, Huan; Yan, Hui; Su, Xiaomin; Liu, Yingquan; Tang, Zenzen; Gao, Yuan; Shang, Wencong; Jiang, Zhou; Wang, Tianze; Che, Yongzhe; Zhang, Yuan; Yang, Rongcun

doi:10.3389/fimmu.2017.01063

Cited by 21 publications

(23 citation statements)

References 48 publications

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“…For gut microbiota analyses, previously reported method was used 58 . Gut microbiota was analyzed by Majorbio Biotechnology Company (Shanghai, China) using primers that target to the V3–V4 regions of 16S rRNA.…”

Section: Methodsmentioning

confidence: 99%

“…Immunostaining was performed according to reported method 58 . Briefly, colon tissues were embedded in OCT compound (Tissue-Tek, Torrance, CA) and frozen over liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…For fluorescent in situ hybridization (FISH), mucus immune-staining was paired with fluorescent in situ hybridization (FISH) in order to analyze bacteria localization at the surface of the intestinal mucosa according to reported method 58 . In brief, the ileum and colonic tissues (proximal colon, second centimeters from the caecum) containing fecal material were placed in methanol-Carnoy’s fixative solution (60% methanol, 30% chloroform, 10% glacial acetic acid) for a minimum of 3 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR and qRT-PCR were performed according to our previous methods 58 . Total RNA was extracted from the cells, tissues and organs using TRIzol reagent (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

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Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut

Wei

Gao

et al. 2020

Commun Biol

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The expansion of Enterobacteriaceae, such as E. coli is a main characteristic of gut inflammation and is related to multiple human diseases. However, how to control these E. coli overgrowth is not well understood. Here, we demonstrate that gut complement factor D (CFD) plays an important role in eliminating E. coli. Increased E. coli, which could stimulate inflammatory macrophages to induce colitis, were found in the gut of CFD deficient mice. We also showed that gut Reg4, which is expressed in gut epithelial cells, stimulated complementmediated attack complexes to eliminate E. coli. Reg4 deficient mice also had increased E. coli. The dominant E. coli were isolated from colitis tissues of mice and found to be sensitive to both CFD-and Reg4-mediated attack complexes. Thus, gut Reg4-and CFD-mediated membrane attack complexes may maintain gut homeostasis by killing inflammatory E. coli.

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Section: Methodsmentioning

confidence: 99%

“…Immunostaining was performed according to reported method 58 . Briefly, colon tissues were embedded in OCT compound (Tissue-Tek, Torrance, CA) and frozen over liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…RT-PCR and qRT-PCR were performed according to our previous methods 58 . Total RNA was extracted from the cells, tissues and organs using TRIzol reagent (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

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Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut

Wei

Gao

et al. 2020

Commun Biol

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“…Quantitative real-time PCR (RT-qPCR) were performed according to methods published previously [23,24]. Total RNA was extracted from the PPFE samples by using RNeasy FFPE Kit (cat.…”

Section: Rna Isolation and Reverse Transcription Pcrmentioning

confidence: 99%

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Tolerogenic probiotics Lactobacillus delbrueckii and Lactobacillus rhamnosus promote anti‐inflammatory profile of macrophages‐derived monocytes of newly diagnosed patients with systemic lupus erythematosus

Javanmardi,

Mahmoudi,

Rafatpanah

et al. 2024

Cell Biochemistry & Function

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Systemic lupus erythematosus (SLE) is known as an autoimmune disorder that is characterized by the breakdown of self‐tolerance, resulting in disease onset and progression. Macrophages have been implicated as a factor in the development of SLE through faulty phagocytosis of dead cells or an imbalanced M1/M2 ratio. The study aimed to investigate the immunomodulatory effects of Lactobacillus delbrueckii and Lactobacillus rhamnosus on M1 and M2 macrophages in new case lupus patients. For this purpose, blood monocytes were collected from lupus patients and healthy people and were cultured for 5 days to produce macrophages. For 48 h, the macrophages were then cocultured with either probiotics or lipopolysaccharides (LPS). Flow cytometry and real‐time polymerase chain reaction were then used to analyze the expression of cluster of differentiation (CD) 14, CD80, and human leukocyte antigen – DR (HLADR) markers, as well as cytokine expression (interleukin [IL]1‐β, IL‐12, tumor necrosis factor α [TNF‐α], IL‐10, and transforming growth factor beta [TGF‐β]). The results indicated three distinct macrophage populations, M0, M1, and M2. In both control and patient‐derived macrophage‐derived monocytes (MDMs), the probiotic groups showed a decrease in CD14, CD80, and HLADR expression compared to the LPS group. This decrease was particularly evident in M0 and M2 macrophages from lupus patients and M1 macrophages from healthy subjects. In addition, the probiotic groups showed increased levels of IL‐10 and TGF‐β and decreased levels of IL‐12, IL1‐β, and TNF‐α in MDMs from both healthy and lupus subjects compared to the LPS groups. Although there was a higher expression of pro‐inflammatory cytokines in lupus patients, there was a higher expression of anti‐inflammatory cytokines in healthy subjects. In general, L. delbrueckii and L. rhamnosus could induce anti‐inflammatory effects on MDMs from both healthy and lupus subjects.

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Gut REG3γ-Associated Lactobacillus Induces Anti-inflammatory Macrophages to Maintain Adipose Tissue Homeostasis

Cited by 21 publications

References 48 publications

Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut

Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut

Untitled

Tolerogenic probiotics Lactobacillus delbrueckii and Lactobacillus rhamnosus promote anti‐inflammatory profile of macrophages‐derived monocytes of newly diagnosed patients with systemic lupus erythematosus

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