Gut mucosal-associated microbiota better discloses inflammatory bowel disease differential patterns than faecal microbiota

Altomare, Annamaria; Putignani, Lorenza; Chierico, Federica Del; Cocca, Silvia; Angeletti, Silvia; Ciccozzi, Massimo; Tripiciano, Costanza; Piccola, Bruno Dalla; Cicala, Michele; Guarino, Michele Pier Luca

doi:10.1016/j.dld.2018.11.021

Cited by 62 publications

(66 citation statements)

References 59 publications

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“…Recently, among the Firmicutes, a predominance of V. dispar has been described in patients affected by IBD and primary sclerosing cholangitis (Altomare et al, 2018). In our study a preponderanceof V. dispar was found in the MM and FM of patients with EII.…”

Section: Discussionsupporting

confidence: 71%

Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries

Romani

Chierico

Chiriaco

et al. 2020

Front. Cell. Infect. Microbiol.

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Section: Discussionsupporting

confidence: 71%

Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries

Romani

Chierico

Chiriaco

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Supporting that hypothesis, most of our results were consistent with published studies with IBD patients. For example, reduced abundance of F. prausnitzii was reported in many studies [22][23][24][25][26][27][28][29]. We found that F. prausnitzii was negatively associated with fecal calprotectin, EPX, and IgA in the current study.…”

Section: Discussionsupporting

confidence: 67%

“…We found that F. prausnitzii was negatively associated with fecal calprotectin, EPX, and IgA in the current study. The Roseburia genus [23,[30][31][32][33] or specific species (e.g., Roseburia intestinalis and Roseburia hominis) [34,35] were significantly decreased in patients with IBD. In our analysis, Roseburia spp.…”

Section: Discussionmentioning

confidence: 95%

“…Additionally, fecal levels of several commensal bacteria, including Veillonella spp., E. coli, and Fusobacterium spp., were positively associated with inflammation biomarkers in the current study. All of those bacteria were considered as invasive [38][39][40], and high levels were reported previously in IBD patients [23,28,31,33,[41][42][43][44][45]. Changes in Prevotella spp.…”

Section: Discussionmentioning

confidence: 96%

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Development of an Index Score for Intestinal Inflammation-Associated Dysbiosis Using Real-World Stool Test Results

et al. 2019

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Background Gut microbiota play an important role in human health. However, the application of gut microbiome in regular clinical practice is limited by interindividual variations and complexity of test results. Hypothesis It is possible to address interindividual variation by using large data-based exploratory-pattern analysis. Methods The current study was conducted using a large data set (n = 173,221) of nonselective incoming patients' test results from a stool test. The data set included assays for the detection of 24 selected commensal microorganisms and multiple biomarkers in feces. Patients were grouped based on their levels of inflammation biomarkers such as calprotectin, eosinophil protein X, and IgA. Group mean values of biomarkers and commensal microbes were used in an exploratory-pattern analysis for association from which an index score for intestinal inflammation-associated dysbiosis (IAD) was developed. The IAD score was evaluated in one questionnaire-based study (n = 7263) and one prospective case series study (n = 122) with patients of inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease. Results We identified a microbial profile strongly associated with fecal inflammation biomarkers. Developed on the pattern of the microbial profile, the IAD score demonstrated a strong association with fecal inflammation biomarkers and was significantly different between patients with IBD and those with IBS or celiac disease. Conclusion Using real-world data, we have developed a method to predict gut dysbiosis associated with different GI disease conditions. It may help clinicians simplify the process of interpreting gut microbial status and provide gut health assessment and treatment evaluation.

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“…Several studies [5][6][7] have reported that faecal microbes may not accurately represent the engraftment colony in the gastrointestinal tract. The microbiota of the intestinal mucosa and faeces as well as their relevance to diseases are largely different.…”

Section: Introductionmentioning

confidence: 99%

Intestinal barrier breakdown and mucosal microbiota disturbance in neuromyelitis optical spectrum disorder

Chu

Tan

et al. 2020

Preprint

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Background and purpose The mechanism underlying the pathology of neuromyelitis optica spectrum disorder (NMOSD) remains unclear even though increased expression of the water channel protein aquaporin-4 (AQP4) on astrocytes plays an important role. Our previous study revealed that dysbiosis was detected in the faecal microbiota of NMOSD patients. In this study, we further investigated whether the intestinal barrier and mucosal flora balance were also interrupted in NMOSD patients. Methods Sigmoid mucosal biopsies were collected via endoscopy from six patients with NMOSD and compared with those from three patients with multiple sclerosis (MS) and five healthy controls (HCs). These samples were processed for electron microscopy and immunohistochemistry to investigate changes in ultrastructure and in the number and size of intestinal inflammatory cells. Changes in mucosal flora were also analysed by high-throughput 16S ribosomal RNA gene amplicon sequencing. Results The intercellular space between epithelia of the colonic mucosa became wider in MS and NMOSD patients compared to the HCs (P < 0.01), and the expression of tight junction proteins in MS and NMOSD patients significantly decreased compared to that in the HCs. Activation of microphages with many inclusions inside the cytoplasm and enlarged plasmocytes with more particles were found in the NMOSD group. Quantitative analysis showed that the percentage of small-size CD 38 + and CD138 + cells was lower but that of larger-size cells became higher in NMOSD patients, and 16S data showed that the abundance of Streptococcus and Granulicatella was dramatically increased in NMOSD patients. Conclusions NMOSD patients exhibited a disrupted intestinal barrier and intestinal dysbiosis and activation of intestinal inflammation, which suggested a potential pathophysiological mechanism of NMOSD underlying intestinal inflammation.

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Gut mucosal-associated microbiota better discloses inflammatory bowel disease differential patterns than faecal microbiota

Cited by 62 publications

References 59 publications

Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries

Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries

Development of an Index Score for Intestinal Inflammation-Associated Dysbiosis Using Real-World Stool Test Results

Intestinal barrier breakdown and mucosal microbiota disturbance in neuromyelitis optical spectrum disorder

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