Gut microbiota profiling of pediatric nonalcoholic fatty liver disease and obese patients unveiled by an integrated meta‐omics‐based approach

Chierico, Federica Del; Nobili, Valério; Vernocchi, Pamela; Russo, Alessandra; Stefanis, Cristiano De; Gnani, Daniela; Furlanello, Cesare; Zandonà, Alessandro; Paci, Paola; Capuani, Giorgio; Dallapiccola, Bruno; Miccheli, Alfredo; Alisi, Anna; Putignani, Lorenza

doi:10.1002/hep.28572

Cited by 572 publications

(514 citation statements)

References 45 publications

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“…Importantly, it has been reported that improved intrahepatic TG content is related to a lower abundance of Firmicutes and a higher abundance of Bacteroidetes (45) (15). In support of these studies, pediatric patients with NAFLD showed an increase in Bradyrhizobium, Anaerococcus, Peptoniphilus, Propionibacterium acnes, Dorea, and Ruminococcus and a decrease in Oscillospira and Rikenellaceae (44). Although it is clear that there may be some discrepancies in what defines dysbiosis in liver disease, the frequency of disease also occurs in association with obesity and is considered a manifestation of metabolic syndrome.…”

Section: Introductionmentioning

confidence: 66%

Nonalcoholic Fatty Liver Disease, the Gut Microbiome, and Diet

Mokhtari

Gibson

Hekmatdoost

2017

Advances in Nutrition

134

100

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Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder in the world, yet the pathogenesis of the disease is not well elucidated. Due to the close anatomic and functional association between the intestinal lumen and the liver through the portal system, it is speculated that the gut microbiome may play a pivotal role in the pathogenesis of NAFLD. Furthermore, diet, which can modulate the gut microbiome and several metabolic pathways involved in NAFLD development, shows a potential tripartite relation between the gut, diet, and the liver. In this review, we summarize the current evidence that supports the association between NAFLD, the gut microbiome, and the role of diet. Adv Nutr 2017;8:240-52.

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Section: Introductionmentioning

confidence: 66%

Nonalcoholic Fatty Liver Disease, the Gut Microbiome, and Diet

Mokhtari

Gibson

Hekmatdoost

2017

Advances in Nutrition

134

100

View full text Add to dashboard Cite

show abstract

“…166 Supporting these studies, pediatric patients with NAFLD had increased numbers of Bradyrhizobium, Anaerococcus, Peptoniphilus, Propionibacterium acnes, Dorea, and Ruminococcus, and decreased numbers of Oscillospira and Rikenellaceae. 167 In another study with adults, patients with NASH harbored a lower abundance of Faecalibacterium and Firmicutes (Clostridiales family, Anaerosporobacter) but a higher abundance of Parabacteroides and Allisonella in their fecal microbiomes. 168 Crucially, it has been reported that improved intrahepatic triglyceride content is related to a lower abundance of Firmicutes and a higher abundance of Bacteroidetes.…”

Section: Nonalcoholic Fatty Liver Diseasementioning

confidence: 99%

Exploring the microbiome in health and disease

Scotti

Boué

Sasso

et al. 2017

Toxicology Research and Application

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The analysis of human microbiome is an exciting and rapidly expanding field of research. In the past decade, the biological relevance of the microbiome for human health has become evident. Microbiome comprises a complex collection of microorganisms, with their genes and metabolites, colonizing different body niches. It is now well known that the microbiome interacts with its host, assisting in the bioconversion of nutrients and detoxification, supporting immunity, protecting against pathogenic microbes, and maintaining health. Remarkable new findings showed that our microbiome not only primarily affects the health and function of the gastrointestinal tract but also has a strong influence on general body health through its close interaction with the nervous system and the lung. Therefore, a perfect and sensitive balanced interaction of microbes with the host is required for a healthy body. In fact, growing evidence suggests that the dynamics and function of the indigenous microbiota can be influenced by many factors, including genetics, diet, age, and toxicological agents like cigarette smoke, environmental contaminants, and drugs. The disruption of this balance, that is called dysbiosis, is associated with a plethora of diseases, including metabolic diseases, inflammatory bowel disease, chronic obstructive pulmonary disease, periodontitis, skin diseases, and neurological disorders. The importance of the host microbiome for the human health has also led to the emergence of novel therapeutic approaches focused on the intentional manipulation of the microbiota, either by restoring missing functions or eliminating harmful roles. In the present review, we outline recent studies devoted to elucidate not only the role of microbiome in health conditions and the possible link with various types of diseases but also the influence of various toxicological factors on the microbial composition and function.

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“…Some bacterial strains, such as Escherichia coli, produce a significant amount of ethanol, which may reach the liver via the bloodstream in a continuous fashion and promote steatosis and liver injury [58]. Several studies assessed microbiome composition and fecal VOC composition in obesity related NAFLD [58][59][60].…”

Section: Non-alcoholic Fatty Liver Diseasementioning

confidence: 99%

“…It was found that 12 fecal VOCS were significantly less, and 18 VOCs more abundant in NAFDL subjects (Table 3). Recently, Del Chierico and colleagues aimed to assess the structural and functional role of gut microbiota in onset and progression of pediatric NAFLD by analysis of fecal microbiota composition and bacterial derived fecal VOCs, by means of GC-MS-SPME [60]. In total, 61 children were included, comprising of 27 (NAFLD), 26 nonalcoholic steatohepatitis (NASH), 8 obese subjects without steatosis and 54 controls.…”

Section: Non-alcoholic Fatty Liver Diseasementioning

confidence: 99%

Application of Fecal Volatile Organic Compound Analysis in Clinical Practice: Current State and Future Perspectives

et al. 2018

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Abstract:Increasing interest is noticed in the potential of volatile organic compound (VOC) analysis as non-invasive diagnostic biomarker in clinical medical practice. The spectrum of VOCs, originating from (patho)physiological metabolic processes in the human body and detectable in bodily excrements, such as exhaled breath, urine and feces, harbors a magnificent source of information. Thus far, the majority of studies have focused on VOC analysis in exhaled breath, aiming at identification of disease-specific VOC profiles. Recently, an increasing number of studies have evaluated the usability of VOC present in the headspace of feces in the diagnostic work-up of a wide range of gastrointestinal diseases. Promising results have been demonstrated particularly in those diseases in which microbiota alterations are considered to play a significant etiological role, such as colorectal carcinoma, inflammatory bowel disease, irritable bowel syndrome, celiac disease and infectious bowel diseases. In addition, fecal VOC analysis seems to have potential as a diagnostic biomarker for extra-intestinal diseases, including bronchopulmonary dysplasia and sepsis. Different methods for VOC analysis have been used in medical studies, such as gas-chromatography mass spectrometry, selected-ion flow tube-mass spectrometry, ion-mobility spectrometry, and electronic nose devices. In this review, the available literature on the potential of fecal VOCs as diagnostic biomarker, including an overview of relevant VOC detection techniques, is discussed. In addition, future hurdles, which need to be taken prior to implementation of VOC analysis in daily clinical practice, are outlined.

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Gut microbiota profiling of pediatric nonalcoholic fatty liver disease and obese patients unveiled by an integrated meta‐omics‐based approach

Abstract: An Oscillospira decrease coupled to a 2-butanone up-regulation and increases in Ruminococcus and Dorea were identified as gut microbiota signatures of NAFL onset and NAFL-NASH progression, respectively. (Hepatology 2017;65:451-464).

Cited by 572 publications

References 45 publications

Nonalcoholic Fatty Liver Disease, the Gut Microbiome, and Diet

Nonalcoholic Fatty Liver Disease, the Gut Microbiome, and Diet

Exploring the microbiome in health and disease

Application of Fecal Volatile Organic Compound Analysis in Clinical Practice: Current State and Future Perspectives

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