Gut microbiota profiling in Han Chinese with type 1 diabetes

Huang, Yun; Li, Si Cheng; Hu, Ji; Ruan, Hai Bin; Guo, Hongyu; Zhang, Hong Hong; Wang, Xin; Pei, Yu; Pan, Yang; Chen, Fang

doi:10.1016/j.diabres.2018.04.032

Cited by 81 publications

(72 citation statements)

References 39 publications

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“…All these results suggest that Christensenellaceae played a We also observed that several genera from the Ruminococcaceae family were more abundant in controls than in women with HIP and were negatively correlated with HbA1c. Consistent with previous findings, Ruminococcaceae were depleted in women with GDM in the third trimester of pregnancy [40], in non-pregnant individuals with type 1 diabetes [41] and even in pre-diabetic subjects [36]. However, the opposite result was obtained in some other studies.…”

Section: Discussionsupporting

confidence: 91%

Gut microbiota in early pregnancy among women with Hyperglycaemia vs. Normal blood glucose

Gao

Zhong

Shen

et al. 2020

BMC Pregnancy Childbirth

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Background: Recent studies suggest that there is a link between the gut microbiota and glucose metabolism. This study aimed to compare the gut microbiota during early pregnancy of women with hyperglycymia to those with normal blood glucose. Methods: Gut microbial composition was analysed in 22 women with hyperglycaemia and 28 age-matched healthy controls during their first prenatal visits (< 20 weeks) using high throughput sequencing of the V3-V4 region of the 16S ribosomal RNA gene. Hyperglycemia was diagnosed based on the criteria recommended by the International Association of Diabetes and Pregnancy Study Groups in 2010. Results: Women with hyperglycemia in pregnancy (HIP) had significantly lower microbial richness and diversity compared with healthy pregnant women. The proportions of the Firmicutes and Bacteroidetes phyla and the ratio of Firmicutes:Bacteroidetes were not different between the two groups. We observed that individuals with HIP had an increased abundance of Nocardiaceae, Fusobacteriaceae, etc., whereas healthy controls had significantly higher levels of Christensenellaceae, Clostridiales_vadinBB60_group, Coriobacteriaceae, etc. Similarly, levels of the members of the Ruminococcaceae family, including Ruminococcaceae_UCG-014, Ruminococcaceae_UCG-003, and Ruminococcaceae_ UCG-002, were significantly reduced in the HIP group and were negatively correlated with HbA1c. HbA1c levels were positively correlated with Bacteroidaceae and Enterobacteriaceae and negatively correlated with Christensenellaceae, etc. CRP was positively correlated with the Bacteroidaceae and Fusobacteriaceae families and the Fusobacterium genus. Conclusions: Our study revealed that individuals with HIP have gut microbial dysbiosis and that certain bacterial groups are associated with glucose metabolism during pregnancy. Further study is needed to provide new ideas to control glucose by modifying the gut microbiota.

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Section: Discussionsupporting

confidence: 91%

Gut microbiota in early pregnancy among women with Hyperglycaemia vs. Normal blood glucose

Gao

Zhong

Shen

et al. 2020

BMC Pregnancy Childbirth

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“…Interestingly, several studies have shown that the Firmicutes: Bacteroides ratio was important in metabolism and related to weight [36][37][38]. Huang et al found that this ratio was related to type 1 diabetes mellitus [39]. Furthermore, our study indicated that the relative abundance of two crucial symbiotic bacteria, Bacteroides spp.…”

Section: Discussionsupporting

confidence: 54%

Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats

Yang

et al. 2020

BMC Complement Med Ther

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Background: The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widely been used in the clinic. Recently, other clinical functions of PPM have been widely analyzed. Results: Administration of the water extract from PPM decreased the level of FBG, TC, and TG, and increased the level of FGC, thereby reducing the IR index and improving IR. Furthermore, Western blot analysis revealed that PPM significantly increased GPR43 and AMPK expression when compared with the MOD group, and GPR43, AMPK were known as glucose metabolism-related proteins. In addition, treatment with PPM can restore the balance of gut microbiota by adjusting the relative abundance of bacteria both at the phylum and genus level, and these changes have been reported to be related to IR. Methods: Sprague Dawley (SD) rats were fed a high-fat diet and were gavaged daily with either normal saline solution or PPM for 12 weeks. Major biochemical indexes, such as fasting blood glucose (FBG), fasting glucagon (FGC), total cholesterol (TC), and triglyceride (TG) were measured. Then the protein expression of adenosine 5′monophosphate-activated protein kinase (AMPK) and G protein-coupled receptor 43 (GPR43) was evaluated by using Western blot analysis. Moreover, the composition of gut microbiota was assessed by analyzing 16S rRNA sequences. Conclusions: Our findings showed that PPM reversed the increasing of FBG and the decreasing of IRI, PPM accelerated the expression of glucose metabolism-related proteins and regulated the intestinal microecological balance. Therefore, we hold the opinion that PPM may be an effective option for treating IR.

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“…It should be noted that another beneficial microbe, Clostridiaceae , was also significantly decreased in CHB, LC and HCC cohorts. Clostridiaceae , comprising many short‐chain fatty acid (SCFA)‐producing bacteria, can produce SCFAs such as propionate, acetate and butyrate; while SCFAs have multiple functions in maintaining human health such as acting as special nutrients and energy component for enterocytes, strengthening epithelial barrier function, reducing the level of inflammation and promoting peripheral Treg cell generation, etc A study documented that a decrease in SCFAs would result in increased colonic pH, ammonia production and absorption in the gut, with hyperammonemia being a very important pathogenetic factor in hepatic encephalopathy . Butyrate, a kind of SCFAs, is the major energy source of the intestinal mucosa and plays an important role in human gut health for its beneficial properties such as its immunomodulatory effects .…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota dysbiosis in patients with hepatitis B virus–induced chronic liver disease covering chronic hepatitis, liver cirrhosis and hepatocellular carcinoma

Zeng

Chen

et al. 2019

Journal of Viral Hepatitis

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The information regarding the effect of hepatitis B virus (HBV) infection on gut microbiota and the relationship between gut microbiota dysbiosis and hepatitis B virus-induced chronic liver disease (HBVCLD) is limited. In this study, we aimed at characterizing the gut microbiota composition in the three different stages of hepatitis B virus-induced chronic liver disease patients and healthy individuals. Faecal samples and clinical data were collected from HBVCLD patients and healthy individuals.The 16S rDNA gene amplification products were sequenced. Bioinformatic analysis including alpha diversity and PICRUSt was performed. A total of 19 phyla, 43 classes, 72 orders, 126 families and 225 genera were detected. The beta-diversity showed a separate clustering of healthy controls and HBVCLD patients covering chronic hepatitis (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC); and gut microbiota of healthy controls was more consistent, whereas those of CHB, LC and HCC varied substantially. The abundance of Firmicutes was lower, and Bacteroidetes was higher in patients with CHB, LC and HCC than in healthy controls. Predicted metagenomics of microbial communities showed an increase in glycan biosynthesis and metabolism-related genes and lipid metabolism-related genes in HBVCLD than in healthy individuals. Our study suggested that HBVCLD is associated with gut dysbiosis, with characteristics including, a gain in potential bacteria and a loss in potential beneficial bacteria or genes. Further study of CHB, LC and HCC based on microbiota may provide a novel insight into the pathogenesis of HBVCLD as well as a novel treatment strategy. K E Y W O R D S16S rDNA, dysbiosis, Gut Microbiota, hepatitis B virus, progression

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Gut microbiota profiling in Han Chinese with type 1 diabetes

Cited by 81 publications

References 39 publications

Gut microbiota in early pregnancy among women with Hyperglycaemia vs. Normal blood glucose

Gut microbiota in early pregnancy among women with Hyperglycaemia vs. Normal blood glucose

Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats

Gut microbiota dysbiosis in patients with hepatitis B virus–induced chronic liver disease covering chronic hepatitis, liver cirrhosis and hepatocellular carcinoma

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