Gut microbiota mediates the protective role of Lactobacillus plantarum in ameliorating deoxynivalenol-induced apoptosis and intestinal inflammation of broiler chickens

Yang, Xin; Liang, Saisai; Guo, Fangshen; Ren, Zhouzheng; Long, Fangyu

doi:10.1016/j.psj.2019.10.034

Cited by 47 publications

(48 citation statements)

References 42 publications

(51 reference statements)

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“…However, dietary DON and C. perfringens challenge had an interaction effect on butyric acid content. The changes of apoptosis and intestinal inflammation status are driven by the modified microbiota ( Yang et al., 2020 ). Spearman correlation analysis was used to characterize associations of bacterial relative abundances with SCFAs' concentrations in cecal digesta and mRNA expression of inflammation and apoptosis-relative genes.…”

Section: Discussionmentioning

confidence: 99%

Study on the interactive effect of deoxynivalenol and Clostridium perfringens on the jejunal health of broiler chickens

Guo

Wang

et al. 2021

Poultry Science

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With global warming and ban on antibiotics, it occurs occasionally that deoxynivalenol ( DON ) together with Clostridium perfringens impairs the gut health of broiler chickens. However, the interactive effect of DON and C. perfringens on intestinal health is still unknown. A total of 120 one-day-old Arbor Acres broilers were randomly distributed to 4 groups. Birds were gavaged with C. perfringens (8 × 10 8 CFU/d per bird) or sterile medium and fed a DON diet (0 or 5 mg of DON per kg diet) to investigate the interactive effects. The main effect analysis showed that DON diet significantly downregulated ( P < 0.05) the mRNA expression of mucin-2, B-cell lymphoma-2–associated X, and cysteinyl aspartate–specific proteinase-3 of jejunal mucosa; decreased ( P < 0.05) the indexes of ACE, Chao1, Shannon, and Simpson; and also decreased the relative abundance of the phylum Bacteroidete and the genera Lactococcus in jejunal contents of broilers chickens. Meanwhile, C. perfringens significantly increased ( P < 0.05) crypt depth; decreased ( P < 0.05) the ratio of villi height to crypt depth, the activity of jejunal diamine oxidase, and the relative abundance of Lactococcus ; and upregulated ( P < 0.05) the relative expression of B-cell lymphoma-2 and cysteinyl aspartate–specific proteinase-8. Furthermore, the interactions between DON and C. perfringens were most significant ( P < 0.05) in the mRNA expression of lipopolysaccharide-induced TNF factor ( LITA F ) and TLR-4 , the abundance of the genera Lactococcus in jejunal contents, and butyric acid concentrations in cecal contents of birds. Finally, Spearman correlation analysis suggested that the most negative correlations ( P < 0.05) with the abundance of the genera except Lactobacillus were observed within the mRNA expression of LITAF . The abundance of Lactococcus had a positive correlation ( P < 0.05) with the expression of Caspase-3. Most genera except Lactobacillus negatively correlated ( P < 0.05) with acetic acid, butyric acid, and total short-chain fatty acids. In conclusion, dietary deoxynivalenol and C. perfringens challenge had a harmful effect on the jejunal health and should be carefully monitored in broiler production.

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Section: Discussionmentioning

confidence: 99%

Study on the interactive effect of deoxynivalenol and Clostridium perfringens on the jejunal health of broiler chickens

Guo

Wang

et al. 2021

Poultry Science

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“…This down-regulation of mTOR may be attributed to the reduced amino acids and energy uptake in intestinal cells of C. perfringens -challenged broilers 18 and the reduced VH:CD ratio of C. perfringens -challenged broilers. The mTOR/RPS6KB1 pathway is essential to the intestinal cell migration 19 and thus, could help to accelerate the healing of NE-induced intestinal damage and promote the recovery of tissues. In contrast, Eimeria -challenged birds showed an activation of mTOR c omplex 1 pathways indicating a higher gut cell turnover to reduce the intestinal mucosal disruption.…”

Section: Discussionmentioning

confidence: 99%

Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions

Criado-Mesas

Abdelli

Noce

et al. 2021

Sci Rep

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There is a high interest on gut health in poultry with special focus on consequences of the intestinal diseases, such as coccidiosis and C. perfringens-induced necrotic enteritis (NE). We developed a custom gene expression panel, which could provide a snapshot of gene expression variation under challenging conditions. Ileum gene expression studies were performed through high throughput reverse transcription quantitative real-time polymerase chain reaction. A deep review on the bibliography was done and genes related to intestinal health were selected for barrier function, immune response, oxidation, digestive hormones, nutrient transport, and metabolism. The panel was firstly tested by using a nutritional/Clostridium perfringens model of intestinal barrier failure (induced using commercial reused litter and wheat-based diets without exogenous supplementation of enzymes) and the consistency of results was evaluated by another experiment under a coccidiosis challenge (orally gavaged with a commercial coccidiosis vaccine, 90× vaccine dose). Growth traits and intestinal morphological analysis were performed to check the gut barrier failure occurrence. Results of ileum gene expression showed a higher expression in genes involved in barrier function and nutrient transport in chickens raised in healthy conditions, while genes involved in immune response presented higher expression in C.perfringens-challenged birds. On the other hand, the Eimeria challenge also altered the expression of genes related to barrier function and metabolism, and increased the expression of genes related to immune response and oxidative stress. The panel developed in the current study gives us an overview of genes and pathways involved in broiler response to pathogen challenge. It also allows us to deep into the study of differences in gene expression pattern and magnitude of responses under either a coccidial vaccine or a NE.

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“…Consequently, intestinal microbes might be a source of amino acids by themselves Metges (2000); Rubio (2019). However, the bulk of these microbial proteins are vanished to the host with the defecation as most of the gut microorganisms in chickens live in the cecum which cannot break and absorb protein Yang et al (2019).…”

Section: Nutritional Interactionsmentioning

confidence: 99%

“…The gastrointestinal (GI) tract of chicken is exposed to exogenous microbes instantly after hatch and subsequently, it turns into a suitable environment for dense microbes containing mostly the anaerobic microorganisms. As chicken matures, these microbes become very divergent until it attains a comparatively active state Yang et al (2019); Blanch et al (2020). In comparison to other animals, chicken has a smaller gastrointestinal tract and rapid digesta passage.…”

Section: Introductionmentioning

confidence: 99%

Addition of Alpha-Lipoic Acid in Semen Extender Improves Post-Thaw Antioxidant Profile and Semen Quality of Achai (Bos Indicus) Cattle Bulls

2020

THE JAPS

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The actual functioning of the intestinal tract of chicken and its wellbeing are key elements in determining chicken health and performance. Many complicated mechanisms are engaged in the adaptation of gastrointestinal tract functioning and wellbeing. The intestinal tract of chicken is heavily colonized with microbes, which directly interrelate with the host. The gut microbiota has been displayed to principally influence chicken well-being via several functions in relation to diet, immune response, and other physical indices. Intestinal microbiota assists by supplying nutrients from low nutritious feeds and moderating the growth of gastrointestinal tract and immune functions. In response, host delivers a lenient environment and nutrients for microbial establishment and development. Modifying the intestinal microbiota may help the host in terms of good health and proper functioning. The idea of administering favorable microorganisms to the feed has led to the advancement of feed additives especially prebiotics and probiotics. The intestinal microbiota is quickly variable by feed, antibiotics, contamination by microbes, and other host and environmental factors. The capability to deliberately influence the intestinal microbiota by supplying nutritional elements, moderating host immune system, inhibiting microbial gut establishment, or increasing gut barrier function has led to many new techniques to inhibit disease chances, however, it led to enhanced weight gain, carcass production and feed conversion ratio (FCR). Moreover, the application of genomics (next-generation sequencing platforms and sequence database) will be economical, easy to use, and capable of dealing with the nature of poultry and the food safety requirements.

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Gut microbiota mediates the protective role of Lactobacillus plantarum in ameliorating deoxynivalenol-induced apoptosis and intestinal inflammation of broiler chickens

Cited by 47 publications

References 42 publications

Study on the interactive effect of deoxynivalenol and Clostridium perfringens on the jejunal health of broiler chickens

Study on the interactive effect of deoxynivalenol and Clostridium perfringens on the jejunal health of broiler chickens

Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions

Addition of Alpha-Lipoic Acid in Semen Extender Improves Post-Thaw Antioxidant Profile and Semen Quality of Achai (Bos Indicus) Cattle Bulls

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