Gut Microbiota Mediate Insecticide Resistance in the Diamondback Moth, Plutella xylostella (L.)

Xia, Xiaofeng; Sun, Botong; Gurr, Geoff M.; Vasseur, Liette; Xue, Minqian; You, Minsheng

doi:10.3389/fmicb.2018.00025

Cited by 177 publications

(165 citation statements)

References 52 publications

(76 reference statements)

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“…was more susceptible to the insecticide chlorpyrifos. 63 We have shown here and in our previous work that S. symbiotica can invade various tissues of the pea aphid, as is also the case for Rickettsia spp. in B. tabaci, which increased the sensitivity of the host to insecticides (Table 1).…”

Section: Discussionsupporting

confidence: 71%

See 1 more Smart Citation

Fitness costs of infection with Serratia symbiotica are associated with greater susceptibility to insecticides in the pea aphid Acyrthosiphon pisum

Škaljac

Kirfel

Grotmann

et al. 2018

Pest Management Science

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show abstract

Section: Discussionsupporting

confidence: 71%

“…In addition, P. xylostella infected with gut symbiont Serratia spp. was more susceptible to the insecticide chlorpyrifos . We have shown here and in our previous work that S. symbiotica can invade various tissues of the pea aphid, as is also the case for Rickettsia spp.…”

Section: Discussionmentioning

confidence: 99%

Fitness costs of infection with Serratia symbiotica are associated with greater susceptibility to insecticides in the pea aphid Acyrthosiphon pisum

Škaljac

Kirfel

Grotmann

et al. 2018

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Similarly, numerous investigations have been devoted to elucidating the role of intestinal microflora in mammals (Pasinetti et al, 2018;Roselli et al, 2017) and fishes (Gómez & José Luis, 2010;Zhou, Ringø, Olsen, & Song, 2018). Although studying entomic intestinal microflora remains relatively novel, its development and metabolism, even behavior is shown to be similar to the gut microbiota of the other animals (Ayayee, Muñoz-Garcia, & Keeney, 2018;Qiao, Keesey, Hansson, & Knaden, 2019;Xia et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Zeng

Shi

Guo

et al. 2020

Arch Insect Biochem Physiol

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To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After feeding on the diets with different pH values for 84 hr, fourth‐instar caterpillars were randomly collected. Growth and various physiological traits were determined and 16S recombinant DNA sequencing was performed using the intestinal microflora of surviving larvae. Results showed that the mortality was 30% in DPB6, and 10% in DPB8, while no mortality was observed in control. The partial least squares discriminant analyses suggested that diets prepared with PB of different pH resulted in different food intake, amount of produced feces, weight gain, digestive enzyme activities, and antioxidant enzyme activities in larvae. Interestingly, both the highest weight gain and the lowest total antioxidant capacities were seen in control larvae. Results also showed that the larval gut microbiota community structure was significantly affected by dietary pH. Moreover, linear discriminant analysis effect size suggested that the family Acetobacteraceae in control, genus Prevotella in DPB8, and genus Lactococcus, family Flavobacteriaceae, family Mitochondria, and family Burkholderiaceae in DPB6 contributed to the diversity of the larval gut microbial community.

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“…Previous studies have also identified important roles of bacterial symbionts in the interactions between phytophagous insects and host plants (Frago et al 2012; Biere and Bennett 2013; Brady and White 2013). In addition, microbial symbionts can break down complex molecules such as insecticides and promote insecticide resistance (Kikuchi et al 2012; Xia et al 2018). It is also notable that pathogenic bacteria can reside in host guts, only initiating or facilitating pathogenesis under certain conditions (Wei et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya

Gichuhi

Subramanian

Khamis

et al. 2019

Preprint

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9The invasive fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes 10 widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. 11frugiperda could play a role in their success and adaptability. However, these bacterial communities remain 12 poorly studied, especially for S. frugiperda in Africa. We investigated the composition, abundance and 13 diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four 14 maize growing regions in Kenya through high throughput sequencing of bacterial 16S rRNA gene. We 15 identified Proteobacteria and Firmicutes as the most dominant phyla and lesser proportions of 16 Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between 17 larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during 18 metamorphosis. Several bacterial groups were found in both adults and larvae suggesting that they are 19 transmitted across developmental stages. Reads corresponding to several known entomopathogenic 20 bacterial clades as well as the non-bacterial entomopathogen, Metarhizium rileyi (Farl.) Kepler, Rehner & 21 Humber (2014), were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya 22 indicated the presence of both 'Rice' and 'Corn' strains, with a higher prevalence of the 'Rice' strain. Insights 23 into the microbiota may ultimately provide alternative avenues for controlling of this pest.24

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Gut Microbiota Mediate Insecticide Resistance in the Diamondback Moth, Plutella xylostella (L.)

Cited by 177 publications

References 52 publications

Fitness costs of infection with Serratia symbiotica are associated with greater susceptibility to insecticides in the pea aphid Acyrthosiphon pisum

Fitness costs of infection with Serratia symbiotica are associated with greater susceptibility to insecticides in the pea aphid Acyrthosiphon pisum

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya

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