Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study

Abstract

BACKGROUND: There is limited information on gut microbiota of neonates with congenital gastrointestinal surgical conditions (CGISCs) available. METHODS: This study compared stool microbiota and short-chain fatty acids (SCFAs) of 37 term infants with CGISCs with 36 term healthy infants (HIs). Two stool samples were collected from each infant: as soon as possible after birth (week 1) and 10-14 days of life (week 2). RESULTS: Bacterial richness and alpha diversity were comparable between CGISCs and HIs at week 1 and week 2 (all p > 0.05). Beta diversity analysis revealed that at week 1, CGISCs had similar community structures to HIs (p = 0.415). However, by week 2, community structures of CGISCs were significantly different from HIs (p = 0.003). At week 1, there were no significant differences in the relative abundances of genera Bifidobacterium and Bacteroides between CGISCs and HIs. At week 2, the relative abundance of Bifidobacterium was significantly lower in CGISCs (mean percentage 7.21 ± 13.49 vs. 28.96 ± 19.6; p = 0.002). Bacteroides were also less abundant in the CGISC group (mean percentage 0.12 ± 0.49 vs. 6.59 ± 8.62; p = 0.039). Relative abundance of genera Pseudomonas and Escherichia-Shigella were higher in CGISCs. At week 2, stool concentrations of all SCFAs were lower in CGISCs (all p < 0.001). CONCLUSIONS: During hospitalization, neonates with CGISCs develop gut dysbiosis and deficiency of SCFAs.

Probiotic supplementation in neonates with congenital gastrointestinal surgical conditions: a pilot randomised controlled trial

“…The stool microbiota was assessed using the 16S ribosomal RNA gene sequencing method (Supplementary File 1 ). Stool short-chain fatty acid assay was performed according to our previously described method 13 with slight modifications (Supplementary File 1 ). The scientists who conducted microbial and SCFA assays and their respective statistical analyses were blinded to the randomisation groups.…”

“… 10 – 15 Our previous prospective study found that neonates with CGISC develop gut dysbiosis during their stay in the neonatal intensive care unit. 13 …”

“…Our previous prospective study also found that neonates with CGISC have lower faecal SCFA levels. 13 …”

Physical development and its relationship to the metabolic activity of the intestinal microbiota in young children

“…По мнению Ra S. C. и коллег (2020 г. ), одним из последствий перенесенной резекции части кишечника у детей является нарушение микробиоты с дефицитом Bifidobacteria и Bacteroides, повышенной распространенностью Escherichia, Shigella и Pseudomonas, а также низкий уровень короткоцепочечных жирных кислот (КЖК) в кале в сравнении со здоровыми детьми [12]. Это может способствовать нарушениям функционирования системы органов пищеварения, ассимиляции питательных веществ, оптимальных процессов роста и развития ребенка в целом [13,14].…”

“…Выявлено снижение темпов роста (длина тела SD: от -1до -2) при низких значениях (<25‰) суммы кислот, при нарастании пропионата (˃25‰) в кале. Снижение питания (масса тела SD: от -1до -2) ассоциировалось с низкими значениями суммы изокислот и АИ (-) (<25‰); к 3 годам жизни риск пониженного питания снижался в 4 раза при высоких значениях (˃75‰) суммы кислот в кале (OR=4,33[1,(9)(10)(11)(12)(13)(14)(15)(16)(17)71]). Разнонаправленность содержания КЖК в кале у детей раннего возраста и их влияние на показатели ФР подчеркивает многогранность функций кишечной микробиоты, оказывающей локальное и системное воздействие на организм человека.Ключевые слова: дети раннего возраста, физическое развитие, метаболическая активность кишечника, короткоцепочечные жирные кислоты.…”