Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese Centenarians

Abstract

Increasing evidence suggests that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella, and Akkermansia was significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit the health of centenarians.

Gut microbiota alterations in moderate to severe acne vulgaris patients

“…Here, we used this advanced sequencing technology based on the 16S rRNA sequences in microbial genomes to analyze the gut microbiota in 31 acne patients using 31 paired healthy individuals as the controls. In healthy controls, the commonest phylum was Bacteroidetes, followed by Firmicutes and Proteobacteria, consistent with other studies from Chinese fecal samples 15,17 but different from the results from Americans and Egyptians, in which the commonest phyla were Firmicutes and then Bacteroidetes. 14 The major taxa are related to human colon environment, and the ingredients of diets in different countries may affect the structure of gut microbiota.…”

“…In healthy controls, the commonest phylum was Bacteroidetes, followed by Firmicutes and Proteobacteria, consistent with other studies from Chinese fecal samples but different from the results from Americans and Egyptians, in which the commonest phyla were Firmicutes and then Bacteroidetes . The major taxa are related to human colon environment, and the ingredients of diets in different countries may affect the structure of gut microbiota .…”

The influence of bioregenerative life‐support system dietary structure and lifestyle on the gut microbiota: a 105‐day ground‐based space simulation in Lunar Palace 1

“…The dietary information of the crewmembers was monitored using a food frequency questionnaire (FFQ) before and after 105d‐E (Wang et al ., ). Survey content was divided into 23 kinds of food and food combinations, the food intake ( M ) was calculated as follows: …”

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