Gut microbiota: a new avenue to reveal pathological mechanisms of constipation

Yang, Lei; Wang, Yu; Zhang, Yun; Li, Wenwen; Yang, Lei; Qian, Dawei; Duan, Jin‐Ao

doi:10.1007/s00253-022-12197-2

Cited by 21 publications

(17 citation statements)

References 145 publications

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“…Combined with the results in Figure 6 , it was discovered that the majority of the distinctive bacterial species in the B . longum intervention group were SCFA-producing bacteria (mainly concentrated in Bifidobacteriaceae, Ruminococcaceae, and Lachnospiraceae) [ 46 , 47 , 48 ]. They also play crucial roles in the control of gastrointestinal motility.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Dose–Effect Relationship of Bifidobacterium longum in Relieving Loperamide Hydrochloride-Induced Constipation in Rats through Colon-Released Capsules

Zhou

Mao

Tang

et al. 2023

IJMS

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Constipation is a common disease affecting humans. Bifidobacterium longum is reportedly effective in relieving constipation. Current studies generally focus on the dose–response relationship of oral doses; however, the dose–effect relationship of B. longum in the colon, which is the primary site where B. longum exerts constipation-relieving effects, to treat constipation has not been studied. Herein, three strains of B. longum (FGSZY6M4, FJSWXJ10M2, and FSDJN6M3) were packaged in colon-released capsules to explore the dose–effect relationship in the colon. For each strain, three groups of capsules (104, 106, and 108 CFU/capsule, respectively) and one group of free probiotics (108 CFU/mL) were used to explore the colonic dose effect of B. longum. The results showed that the three strains of B. longum improved fecal water content and promoted intestinal motility by regulating gastrointestinal peptide (MTL, GAS, and VIP), aquaporin-3, and 5-hydroxytryptamine levels while promoting gastrointestinal motility and relieving constipation by regulating the intestinal flora composition of constipated rats and changing their metabolite content (short-chain fatty acids). Among the three free bacterial solution groups (108 CFU/mL), FGSZY6M4 was the most effective in relieving constipation caused by loperamide hydrochloride in rats. The optimal effective dose of each strain was 6M4 (104 CFU/day), 10M2 (106 CFU/day), and S3 (108 CFU/day) of the colon-released capsules. Therefore, for some effective strains, the dose of oral probiotics can be reduced by colon-released capsules, and constipation can be relieved without administering a great number of bacterial solutions. Therefore, investigating the most effective dose of B. longum at the colon site can help to improve the efficiency of relieving constipation.

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Section: Discussionmentioning

confidence: 99%

Exploring the Dose–Effect Relationship of Bifidobacterium longum in Relieving Loperamide Hydrochloride-Induced Constipation in Rats through Colon-Released Capsules

Zhou

Mao

Tang

et al. 2023

IJMS

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“…Additionally, gut microbiota has been demonstrated to be closely related to the progress of constipation . By performing 16S rDNA amplicon sequencing, we uncovered that BEN intervention improved LOP-lowered species richness and evenness and also reshaped LOP-disordered microbial structure in constipated mice.…”

Section: Discussionmentioning

confidence: 90%

“…Additionally, gut microbiota has been demonstrated to be closely related to the progress of constipation. 35 By performing 16S rDNA amplicon sequencing, we uncovered that BEN intervention improved LOP-lowered species richness and evenness and also reshaped LOP-disordered microbial structure in constipated mice. Among those BEN-responsive gut microbes with the abundance altered significantly, Anaerotruncus can increase the production of butyric acid and participate in glucose metabolism, 36 and Bacteroidetes are acetic acid producers and correlated with the absorption of water and electrolytes to relieve constipation.…”

Section: Discussionmentioning

confidence: 94%

Broccoli-Derived Exosome-like Nanoparticles Alleviate Loperamide-Induced Constipation, in Correlation with Regulation on Gut Microbiota and Tryptophan Metabolism

Duan,

Wang,

Dong

et al. 2023

J. Agric. Food Chem.

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Constipation, a common gastrointestinal dysfunction, damages patients' life quality and predisposes them to other serious diseases. Current strategies against constipation often cause drug dependency and side effects. Here, we demonstrated that broccoli-derived exosome-like nanoparticles (BENs), a natural product with high gastrointestinal stability, ameliorated LOP-induced constipation in mice. Specifically, orally administered BENs (17.5 mg/kg/d) effectively shortened defecation time, sped up intestinal propulsion rate, and increased feces amount in constipated mice. BENs also raised excitatory neurotransmitters SP and MTL and reduced inhibitory neurotransmitters VIP and ET-1. Mechanistically, BENs were taken up by gut microbes, restored LOP-disordered gut microbiota, and altered microbial metabolism of SCFAs and tryptophan, as evidenced by the results of fluorescence microscopy, 16S rRNA gene sequencing, and nontargeted metabolomics. Thereinto, BEN-enriched SCFA-producing microorganisms are closely associated with the feces amount and SP and VIP levels and BEN-elevated indole-3-pyruvic acid and 3-indoleacetic acid are highly linked to ET-1, SP, and MTL levels. Conclusively, BENs, mitigating constipation by regulating gut microbiota and microbial tryptophan metabolism, showed high potential to be developed as alternative regimens for constipation.

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“…Therefore, it was reasonable to deduce that DF-BPC upregulated SCFAs biosynthesis primarily through the enrichment of the Butyricimonas and Clostridium genera. Interestingly, these two SCFAs (acetic and propionic acids) are natural ligands for G protein-coupled receptors that regulate gut motility through multiple pathways. , GPR41 and GPR43 are extensively distributed in various colonic cells, including epithelial and neuronal cells. Propionic and butyric acids are natural ligands associated with the activation of free fatty acid receptors such as GPR41 and GPR43. , Consistently, the mRNA expression levels of GPR41 and GPR43 were upregulated in mice treated with DF-BPC, relative to those in constipated mice.…”

Section: Discussionmentioning

confidence: 99%

Lychee Pulp-Derived Dietary Fiber-Bound Phenolic Complex Upregulates the SCFAs-GPRs-ENS Pathway and Aquaporins in Loperamide-Induced Constipated Mice by Reshaping Gut Microbiome

Dong,

Xu,

Huang

et al. 2023

J. Agric. Food Chem.

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This study aimed to investigate the effects of the lychee pulp-derived dietary fiber-bound phenolic complex (DF-BPC) on a murine model of loperamide-induced constipation and its molecular mechanism associated with gut microbiota modification. DF-BPC supplementation mitigated loperamide-induced dyschezia, intestinal hypomotility, and colonic impairment, as evidenced by the increased gastro-intestinal transit rate and mucus cell counts. By comparison, short-chain fatty acids (SCFAs) contents and relative abundances of associated genera (Butyricimonas, Clostridium, and Lactobacillus) were effectively upregulated following DF-BPC supplementation. Notably, DF-BPC significantly enhanced expressions of G protein-coupled receptor (GPR) 41 and 43, reaching 1.43-and 1.62-fold increase, respectively. Neurotransmitter secretions were simultaneously altered in DF-BPCtreated mice, suggesting upregulation of the SCFAs-GPRs-enteric nervous system pathway. The overexpression of aquaporins (AQP3, 8, and 9) was stimulated partly through GPRs activation. Mild inflammation associated with constipation was inhibited by suppressing LBP-TLR4-NF-κB signaling translocation. These findings suggest that DF-BPC from lychee pulp has the potential to alleviate constipation in mice through modifying the gut microbiome.

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Gut microbiota: a new avenue to reveal pathological mechanisms of constipation

Cited by 21 publications

References 145 publications

Exploring the Dose–Effect Relationship of Bifidobacterium longum in Relieving Loperamide Hydrochloride-Induced Constipation in Rats through Colon-Released Capsules

Exploring the Dose–Effect Relationship of Bifidobacterium longum in Relieving Loperamide Hydrochloride-Induced Constipation in Rats through Colon-Released Capsules

Broccoli-Derived Exosome-like Nanoparticles Alleviate Loperamide-Induced Constipation, in Correlation with Regulation on Gut Microbiota and Tryptophan Metabolism

Lychee Pulp-Derived Dietary Fiber-Bound Phenolic Complex Upregulates the SCFAs-GPRs-ENS Pathway and Aquaporins in Loperamide-Induced Constipated Mice by Reshaping Gut Microbiome

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