Gut microbiome dysbiosis in patients with hepatitis B virus-related hepatocellular carcinoma after extended hepatectomy liver failure

Abstract

Background: Hepatitis B virus-related hepatocellular carcinoma (B-HCC) negatively affects the gut microbiome. This study aimed to investigate the gut microbiome profiles and functions post-hepatectomy liver failure (PHLF) after extended hepatectomy (e-PHLF) to obtain valuable insights, identify potential diagnostic biomarkers, and assist in the treatment of this disease.Methods: B-HCC patients who underwent extended hepatectomy were consecutively recruited and divided into Group A (n=15) and Group B (n=15) based on the presence and absence of e-PHLF, respectively.The relationships between gut microbiota and extended hepatectomy liver failure were explored using 16S ribosomal RNA (16S rRNA) gene sequencing data.Results: Following extended hepatectomy, the α-diversity of Group A was significantly higher than that of Group B (Shannon P=0.034 or Simpson P=0.031), and the β-diversity differed significantly between Groups A and B (P=0.004, R=0.100). At the genus level, 10 bacterial genera (Bacteroides, Pantoea, Methylobacterium-Methylorubrum, Inquilinus, Mycobacterium, Allisonella, Helicobacter, GCA-900066575, IS-44, and Faecalibacterium) were significantly enriched in Group A, whereas five genera (Papillibacter, Scardovia, Turicibacter, Catabacter, and Senegalimassilia) were significantly enriched in Group B. The highly abundant genera Bacteroides, Pantoea, Faecalibacterium, and Turicibacter participated in multiple amino acid metabolism pathways, organic acid metabolism pathways, pyrimidine metabolism pathways, palmitate biosynthesis, and stearate biosynthesis. Redundancy analysis showed that four environmental factors (total bilirubin, international normalized ratio, prealbumin, and albumin) were significantly correlated with intestinal microorganisms. The formation of interaction networks between different gut microbiomes revealed important correlations between the gut microbiome, and there was a significant correlation between the highly abundant gut microbiome and main functions. Conclusions:The gut microbiota characteristics in B-HCC patients after extended hepatectomy liver failure might allow for the use of non-invasive biomarkers for disease diagnosis and treatment.

Cyanidin-3-O-Glucoside Alleviates Alcoholic Liver Injury via Modulating Gut Microbiota and Metabolites in Mice

“…Yeast β-glucan had a significant impact on reducing the abundance of Bilophila in human cohorts, a microorganism associated with obesity. Methylobacterium-Methylorubrum was significantly enriched in post-hepatectomy liver failure [ 59 ]. The interactions among bile acid composition, gut microbiota, and metabolic phenotype were confirmed in the healthy and high-fat diet mice [ 60 ].…”

Alterations of the gut microbiota associated with the occurrence and progression of viral hepatitis

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“…Note that all the records concerning HAV and HDV were excluded (Figure 1). Among the 12 included studies from PubMed, 1 was about HEV infection (Wu et al, 2020), 4 were about HCV infection (Aly et al, 2016;Taylor et al, 2020;Sultan et al, 2021;Ali et al, 2023), and 7 were HBV-related liver disorders (Wang et al, 2017;Liu et al, 2018;Liu et al, 2019;Ni et al, 2019;Chen et al, 2020;Zheng et al, 2020;Li et al, 2022). The remaining unpublished data was from BioProject and concerned HBV (BioProject accession number PRJEB32568).…”

Microbial influence on liver regeneration: understanding gut microbiota and hepatic recovery post partial hepatectomy

“…However, this study revealed a potential remedy: the restoration of specific microbial taxa capable of producing SCFAs, particularly acetate, effectively rescued liver regeneration in mice. This finding indicates potential implications for humans undergoing liver resection procedures, namely, that gut dysbiosis could adversely affect the regeneration process and subsequent patient survival ( 10 , 11 ).…”