Gut microbiome and serum metabolome analyses identify biomarkers associated with sexual maturity in quails

Xiong, Xinwei; Xu, Jiguo; Yan, Xiao; Wu, Shuoshuo; Ma, Jinge; Wang, Zhangfeng; He, Qiaojun; Gong, Jishang; Rao, Yanghui

doi:10.1016/j.psj.2023.102762

Cited by 4 publications

(4 citation statements)

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“…Akkermansia spp. and Erysipelaceae UGG were the units with significantly increased abundance in the microbial taxa of a healthy aging population [27] . Monitoring data on immune function, blood pressure, and body weight in both groups also illustrated the role of quantitative changes in taxa such as Ruminococcus, Prevotella, Oscellobacter CAG, and Bacteroides in the gut microbiome in healthy aging [28] .…”

Section: Gut Microbiota As Predictive Biomarkersmentioning

confidence: 97%

“…and Erysipelaceae UGG were the units with significantly increased abundance in the microbial taxa of a healthy aging population [ 27 ] . Monitoring data on immune function, blood pressure, and body weight in both groups also illustrated the role of quantitative changes in taxa such as Ruminococcus , Prevotella , Oscellobacter CAG, and Bacteroides in the gut microbiome in healthy aging [ 28 ] . Notably, a study has confirmed the feasibility of using altered Sartorius spp./ Pasteurella ratios as an essential microbial alteration for aging-associated sarcopenia; the role of gut bacteria-derived metabolites in characterizing aging-induced neurodegeneration has also been highlighted in another study [ 29 ] , which inspired us to construct a subsystem of aging-related diseases with associated microbial factors for a more precise aging representation.…”

Section: Biological or Clinical Perspectives To Define And Characteri...mentioning

confidence: 99%

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Gut microbiota: an ideal biomarker and intervention strategy for aging

Xu,

Wei

et al. 2024

Microbiome Res Rep

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Population aging is a substantial challenge for the global sanitation framework. Unhealthy aging tends to be accompanied by chronic diseases such as cardiovascular disease, diabetes, and cancer, which undermine the welfare of the elderly. Based on the fact that aging is inevitable but retarding aging is attainable, flexible aging characterization and efficient anti-aging become imperative for healthy aging. The gut microbiome, as the most dynamic component interacting with the organism, can affect the aging process through its own structure and metabolites, thus holding the potential to become both an ideal aging-related biomarker and an intervention strategy. This review summarizes the value of applying gut microbiota as aging-related microbial biomarkers in diagnosing aging state and monitoring the effect of anti-aging interventions, ultimately pointing to the future prospects of microbial intervention strategies in maintaining healthy aging.

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Section: Gut Microbiota As Predictive Biomarkersmentioning

confidence: 97%

Section: Biological or Clinical Perspectives To Define And Characteri...mentioning

confidence: 99%

Gut microbiota: an ideal biomarker and intervention strategy for aging

Xu,

Wei

et al. 2024

Microbiome Res Rep

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“…As described in our previous study [13], the untargeted metabolomics was used to determine the metabolomic profiles of all 22 quail serum samples. A mixture of 400 µL precooled methanol and 100 µL quail serum was vortexed.…”

Section: Metabolomic Profile Determination Of Quail Serum Samplesmentioning

confidence: 99%

“…To date, few studies have concentrated on the alterations in the gut microbiome during quail sexual maturity. Our previous research identified five species that were abundant in the day 20 group (e.g., Enterococcus faecalis) and 12 species that were prevalent in the day 70 group (e.g., Bacteroides neonati and Christensenella massiliensis) as key bacterial markers for quail sexual maturity [13]. However, the quail bacteriophage interactions, bacteria-phage dynamics, and bacteriophageserum metabolites interactions during sexual maturity transition remains unreported.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of intestinal bacteriophages and their relationship with Bacteria and serum metabolites during quail sexual maturity transition

Xiong,

Gong,

et al. 2024

BMC Vet Res

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Background Bacteriophages are prokaryotic viruses that rank among the most abundant microbes in the gut but remain among the least understood, especially in quails. In this study, we surveyed the gut bacteriophage communities in 22 quails at different ages (days 20 and 70) using shotgun metagenomic sequencing. We then systematically evaluated the relationships with gut bacteria and host serum metabolites. Results We discovered that Myoviridae and Siphoviridae were the dominant bacteriophage families in quails. Through a random forest and LEfSe analysis, we identified 23 differential bacteriophages with overlapping presence. Of these, 21 bacteriophages (e.g., Enterococcus phage IME-EFm5 and Enterococcus phage IME-EFm1) showed higher abundances in the day 20 group, while two bacteriophages (Bacillus phage Silence and Bacillus virus WPh) were enriched in the day 70 group. These key bacteriophages can serve as biomarkers for quail sexual maturity. Additionally, the differential bacteriophages significantly correlated with specific bacterial species and shifts in the functional capacities of the gut microbiome. For example, Enterococcus phages (e.g., Enterococcus phage EFP01, Enterococcus phage IME-EFm5, and Enterococcus phage IME-EFm1) were significantly (P < 0.001, FDR) and positively correlated with Enterococcus faecalis. However, the relationships between the host serum metabolites and either bacteriophages or bacterial species varied. None of the bacteriophages significantly (P > 0.05, FDR) correlated with nicotinamide riboside and triacetate lactone. In contrast, some differential bacterial species (e.g., Christensenella massiliensis and Bacteroides neonati) significantly (P < 0.05, FDR) correlated with nicotinamide riboside and triacetate lactone. Furthermore, characteristic successional alterations in gut bacteriophages, bacteria, and host serum metabolites across different ages highlighted a sexual maturity transition coexpression network. Conclusion This study improves our understanding of the gut bacteriophage characteristics in quails and offers profound insights into the interactions among gut bacteriophages, bacteria, and host serum metabolites during the quail’s sexual maturity transition.

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