Gut Microbiome 16S rRNA Gene Amplicon Taxonomic Profiling of Hospitalized Moroccan COVID-19 Patients

Sehli, Sofia; Azami, Abdellah Idrissi; Dini, N.; Habib, Nihal; Chaouni, Bouchra; Hamdi, Salsabil; Idrissi, Najib Al; Amzazi, Saaïd; Allali, Imane; Nejjari, Chakib; Ghazal, Hassan

doi:10.1128/mra.00256-22

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…After removing duplicate studies, screening the title and abstract, and performing a full-text assessment according to the eligibility criteria, 27 studies were finally included in this systematic review (Figure 1). Of the 27 studies, 18 studies compared only COVID-19 patients and HCs, [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] six compared only recovered COVID-19 patients and HCs, [25][26][27][28][29][30] and three compared both COVID-19 and recovered COVID-19 patients to HCs [31][32][33] (Table 1, Tables S3 and S4). Twenty-two studies were conducted in Asia (17 in China [including 4 in Hong Kong SAR], two in Japan, and one each in United Arab Emirates, India, and Bangladesh), while five others were 1, Table S5).…”

Section: Study Selection and Study Characteristicsmentioning

confidence: 99%

A systematic review of gut microbiota profile in COVID‐19 patients and among those who have recovered from COVID‐19

Simadibrata

Lesmana

Gunawan

et al. 2023

J of Digest Diseases

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Given the scale and persistence of coronavirus disease 2019 (COVID-19), significant attention has been devoted to understanding the relationship between human gut microbiota and COVID-19. In this systematic review we aimed to comprehensively assess the gut microbiota composition in patients infected with COVID-19 and those recovered from COVID-19 in comparison to healthy controls (HCs). Methods: Peer-reviewed articles and preprints published up to September 1, 2022, were searched in Ovid MEDLINE, Ovid EMBASE, and SCOPUS. Observational studies reporting the gut microbiota profile in adult (≥18 years) COVID-19 patients or those recovered from COVID-19 compared to HCs were eligible for inclusion in this systematic review. The quality assessment of studies was performed using the Newcastle-Ottawa scale. Results: We identified 27 studies comprising 18 studies that compared COVID-19 patients and six that compared recovered COVID-19 patients to HCs, while the other three studies compared both COVID-19 and recovered COVID-19 patients to HCs. Compared to HCs, decreased gut microbial diversity and richness and a distinctive microbial composition were reported in COVID-19 patients and recovered COVID-19 patients. In COVID-19 patients, Bacteroidetes were found to be enriched, and Firmicutes depleted. Decreased short-chain fatty acid (SCFA)-producing bacteria, such as Faecalibacterium, Ruminococcus, and Bifidobacterium, among others, were also observed in COVID-19 patients, which were not restored to normal levels in those who recovered. Conclusion: Gut dysbiosis was evident in COVID-19, and available data suggested that dysbiosis persisted even in recovered COVID-19 patients, with decreased Firmicutes and SCFA-producing bacteria.

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Section: Study Selection and Study Characteristicsmentioning

confidence: 99%

A systematic review of gut microbiota profile in COVID‐19 patients and among those who have recovered from COVID‐19

Simadibrata

Lesmana

Gunawan

et al. 2023

J of Digest Diseases

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Microbiome and the COVID-19 pandemic

Patel,

Bhattacharjee

2024

Microbes, Microbial Metabolism, and Mucosal Immunity

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Identification of the intestinal microbiota: Methods and applications to Moroccan studies

Semlali,

Benazzouz,

Filali-Zegzouti

et al. 2024

E3S Web Conf.

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The gut microbiota (GM) is a complex microbial community that exerts a considerable influence on human health, and its accurate characterisation has become essential to understanding its roles and implications in various medical conditions. Recent technological advances have paved the way for analytical methods that can uncover this complex microbial community. For these reasons, this review describes the methods used by research teams to discover and understanding the impact of GM in our environment. This paper explores the first phase of collecting and preserving samples, highlighting the impact of storage methods on sample stability and the reliability microbiological analyses. Then, it examines various methods for analysing GM. This diversity of approaches adapted to the specific objectives of each study, whether to characterise diversity using metagenomics, quantify specific micro-organisms using real-time PCR, or use techniques based on microbial targets. These methodologies promise new perspectives for understanding the physiological impact of the microbiota. Finally, concrete examples from Moroccan studies illustrate the application of these techniques to the characterisation of GM. By providing practical advice, this article guides researchers in the judicious choice of methods adapted to their research objectives and budgetary constraints, thereby contributing to the advancement of knowledge about the GM.

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Gut Microbiome 16S rRNA Gene Amplicon Taxonomic Profiling of Hospitalized Moroccan COVID-19 Patients

Cited by 4 publications

References 12 publications

A systematic review of gut microbiota profile in COVID‐19 patients and among those who have recovered from COVID‐19

A systematic review of gut microbiota profile in COVID‐19 patients and among those who have recovered from COVID‐19

Microbiome and the COVID-19 pandemic

Identification of the intestinal microbiota: Methods and applications to Moroccan studies

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