Gut microbial characteristical comparison reveals potential anti-aging function of Dubosiella newyorkensis in mice

Liu, Tianhao; Wang, Juan; Zhang, Chen-yang; Zhao, Lin; Sheng, Ying-yue; Tao, Guo-shui; Xue, Yu-zheng

doi:10.3389/fendo.2023.1133167

Cited by 20 publications

(10 citation statements)

References 41 publications

(41 reference statements)

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“…27 The prominent strain Dubosiella inhabiting the intestinal tract can metabolize compounds to produce butyric acid, which possesses the beneficial effect of enhancing intestinal immunity and promoting host resistance to inflammation. 28,29 Akkermansia, recognized as a beneficial bacterium residing in the gut, has been linked to pathophysiological processes such as inflammation, obesity, and other disease states. 30−32 Importantly, studies employing pglyrp-deficient mice have demonstrated the colitis-protective characteristics of Alistipes, where it exerts mitigating effects on DSS-induced colitis.…”

Section: Discussionmentioning

confidence: 99%

“…Increased abundance of Lachnoclostridium has been observed in patients diagnosed with ulcerative colitis and displaying inflammatory bowel lesions, establishing it as a potential microbial marker for the noninvasive detection of colorectal tumors and colon cancer . The prominent strain Dubosiella inhabiting the intestinal tract can metabolize compounds to produce butyric acid, which possesses the beneficial effect of enhancing intestinal immunity and promoting host resistance to inflammation. , Akkermansia , recognized as a beneficial bacterium residing in the gut, has been linked to pathophysiological processes such as inflammation, obesity, and other disease states. − Importantly, studies employing pglyrp-deficient mice have demonstrated the colitis-protective characteristics of Alistipes , where it exerts mitigating effects on DSS-induced colitis . Furthermore, Lactobacillus has been identified as a key player in the inhibition of DSS-induced colonic inflammation in murine models, presumably through the activation of the AhR pathway, exhibiting regulatory functions on immune responses and nutrient metabolism .…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Gut Microbial Metabolism by Cyanidin-3-O-Glucoside in Mitigating Polystyrene-Induced Colonic Inflammation: Insights from 16S rRNA Sequencing and Metabolomics

Chen,

Zheng,

Yan

et al. 2024

J. Agric. Food Chem.

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Microplastics derived from plastic waste have emerged as a pervasive environmental pollutant with potential transfer and accumulation through the food chain, thus posing risks to both ecosystems and human health. The gut microbiota, tightly intertwined with metabolic processes, exert substantial influences on host physiology by utilizing dietary compounds and generating bacterial metabolites such as tryptophan and bile acid. Our previous studies have demonstrated that exposure to microplastic polystyrene (PS) disrupts the gut microbiota and induces colonic inflammation. Meanwhile, intervention with cyanidin-3-Oglucoside (C3G), a natural anthocyanin derived from red bayberry, could mitigate colonic inflammation by reshaping the gut bacterial composition. Despite these findings, the specific influence of gut bacteria and their metabolites on alleviating colonic inflammation through C3G intervention remains incompletely elucidated. Therefore, employing a C57BL/6 mouse model, this study aims to investigate the mechanisms underlying how C3G modulates gut bacteria and their metabolites to alleviate colonic inflammation. Notably, our findings demonstrated the efficacy of C3G in reversing the elevated levels of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and the upregulation of mRNA expression (Il-6, Il-1β, and Tnf-α) induced by PS exposure. Meanwhile, C3G effectively inhibited the reduction in levels (IL-22, IL-10, and IL-4) and the downregulation of mRNA expression (Il-22, Il-10, and Il-4) of anti-inflammatory cytokines induced by PS exposure. Moreover, PS-induced phosphorylation of the transcription factor NF-κB in the nucleus, as well as the increased level of protein expression of iNOS and COX-2 in the colon, were inhibited by C3G. Metabolisms of gut bacterial tryptophan and bile acids have been extensively implicated in the regulation of inflammatory processes. The 16S rRNA high-throughput sequencing disclosed that PS treatment significantly increased the abundance of pro-inflammatory bacteria (Desulfovibrio, norank_f_Oscillospiraceae, Helicobacter, and Lachnoclostridium) while decreasing the abundance of antiinflammatory bacteria (Dubosiella, Akkermansia, and Alistipes). Intriguingly, C3G intervention reversed these pro-inflammatory changes in bacterial abundances and augmented the enrichment of bacterial genes involved in tryptophan and bile acid metabolism pathways. Furthermore, untargeted metabolomic analysis revealed the notable upregulation of metabolites associated with tryptophan metabolism (shikimate, L-tryptophan, indole-3-lactic acid, and N-acetylserotonin) and bile acid metabolism (3b-hydroxy-5-cholenoic acid, chenodeoxycholate, taurine, and lithocholic acid) following C3G administration. Collectively, these findings shed new light on the protective effects of dietary C3G against PS exposure and underscore the involvement of specific gut bacterial metabolites in the amelioration of colonic inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Modulation of Gut Microbial Metabolism by Cyanidin-3-O-Glucoside in Mitigating Polystyrene-Induced Colonic Inflammation: Insights from 16S rRNA Sequencing and Metabolomics

Chen,

Zheng,

Yan

et al. 2024

J. Agric. Food Chem.

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“…Each of these taxa have been previously identified as inversely associated with obese phenotypes [ 51 , 52 , 53 , 54 , 55 , 56 ]. The utility of these taxa in promoting metabolic health directly has been explored via probiotic potential; supplementation with D. newyorkensis has demonstrated improved endothelial function in mice [ 57 , 58 ], and clinical trials with A. muciniphila have shown improvement in markers of MetS including insulin sensitivity and hypercholesterolemia [ 11 , 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Polyphenols Support Akkermansia muciniphila Growth via Mediation of the Gastrointestinal Redox Environment

Van Buiten,

Seitz,

Metcalf

et al. 2024

Antioxidants

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Obesity and metabolic dysfunction have been shown to be associated with overproduction of reactive oxygen species (ROS) in the gastrointestinal (GI) tract, which contributes to dysbiosis or imbalances in the gut microbiota. Recently, the reversal of dysbiosis has been observed as a result of dietary supplementation with antioxidative compounds including polyphenols. Likewise, dietary polyphenols have been associated with scavenging of GI ROS, leading to the hypothesis that radical scavenging in the GI tract is a potential mechanism for the reversal of dysbiosis. The objective of this study was to investigate the relationship between GI ROS, dietary antioxidants and beneficial gut bacterium Akkermansia muciniphila. The results of this study demonstrated A. muciniphila to be a discriminant microorganism between lean (n = 7) and obese (n = 7) mice. The relative abundance of A. muciniphila was also found to have a significant negative correlation with extracellular ROS in the GI tract as measured using fluorescent probe hydroindocyanine green. The ability of the dietary antioxidants ascorbic acid, β-carotene and grape polyphenols to scavenge GI ROS was evaluated in tandem with their ability to support A. muciniphila bloom in lean mice (n = 20). While the relationship between GI ROS and relative abundance of A. muciniphila was conserved in lean mice, only grape polyphenols stimulated the bloom of A. muciniphila. Analysis of fecal antioxidant capacity and differences in the bioavailability of the antioxidants of interest suggested that the poor bioavailability of grape polyphenols contributes to their superior radical scavenging activity and support of A. muciniphila in comparison to the other compounds tested. These findings demonstrate the utility of the GI redox environment as a modifiable therapeutic target in the treatment of chronic inflammatory diseases like metabolic syndrome.

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“…Previous studies have shown that interventions with beneficial substances can reshape the gut microbiota in colitis mice, leading to significant changes in certain bacterial genera [ 37 , 38 ]. In our study, we observed that compared to DSS-induced model mice, treatment with heat-killed S. boulardii increased the abundance of beneficial bacterial genera, including Dubosiella [ 39 ], Ligilactobacillus [ 40 , 41 ], Turicibacter [ 42 , 43 ], and Ileibacterium [ 44 ]. The growths of these genera were significantly suppressed in DSS model mice, indicating that heat-killed S. boulardii intervention can regulate the gut microbiota by restoring the abundance of dominant bacteria, thus achieving a new balance.…”

Section: Discussionmentioning

confidence: 99%

Heat-Killed Saccharomyces boulardii Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Restoring the Intestinal Barrier, Reducing Inflammation, and Modulating the Gut Microbiota

Jin,

Wu,

Huang

et al. 2024

Nutrients

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Ulcerative colitis (UC) is a global intestinal disease, and conventional therapeutic drugs often fail to meet the needs of patients. There is an urgent need to find efficient and affordable novel biological therapies. Saccharomyces boulardii has been widely used in food and pharmaceutical research due to its anti-inflammatory properties and gut health benefits. However, there is still a relatively limited comparison and evaluation of different forms of S. boulardii treatment for UC. This study aimed to compare the therapeutic effects of S. boulardii, heat-killed S. boulardii, and S. boulardii β-glucan on UC, to explore the potential of heat-killed S. boulardii as a new biological therapy. The results demonstrate that all three treatments were able to restore body weight, reduce the disease activity index (DAI), inhibit splenomegaly, shorten colon length, and alleviate histopathological damage to colonic epithelial tissues in DSS-induced colitis mice. The oral administration of S. boulardii, heat-killed S. boulardii, and S. boulardii β-glucan also increased the levels of tight junction proteins (Occludin and ZO-1), decreased the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the serum, and suppressed the expressions of TNF-α, IL-1β, and IL-6 mRNA in the colon. In particular, in terms of gut microbiota, S. boulardii, heat-killed S. boulardii, and S. boulardii β-glucan exhibited varying degrees of modulation on DSS-induced dysbiosis. Among them, heat-killed S. boulardii maximally restored the composition, structure, and functionality of the intestinal microbiota to normal levels. In conclusion, heat-killed S. boulardii showed greater advantages over S. boulardii and S. boulardii β-glucan in the treatment of intestinal diseases, and it holds promise as an effective novel biological therapy for UC. This study is of great importance in improving the quality of life for UC patients and reducing the burden of the disease.

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Gut microbial characteristical comparison reveals potential anti-aging function of Dubosiella newyorkensis in mice

Cited by 20 publications

References 41 publications

Modulation of Gut Microbial Metabolism by Cyanidin-3-O-Glucoside in Mitigating Polystyrene-Induced Colonic Inflammation: Insights from 16S rRNA Sequencing and Metabolomics

Modulation of Gut Microbial Metabolism by Cyanidin-3-O-Glucoside in Mitigating Polystyrene-Induced Colonic Inflammation: Insights from 16S rRNA Sequencing and Metabolomics

Dietary Polyphenols Support Akkermansia muciniphila Growth via Mediation of the Gastrointestinal Redox Environment

Heat-Killed Saccharomyces boulardii Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Restoring the Intestinal Barrier, Reducing Inflammation, and Modulating the Gut Microbiota

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