Gut microbial and metabolic characterization of Atlantic salmon (Salmon salar) challenged with Aeromonas salmonicida

Liu, Yanyun; Li, Xiaohao; Xia, Yuqing; Cheng, Jianxin; Zhou, Cheng; Liu, Pengfei

doi:10.1016/j.aquaculture.2023.739420

Cited by 2 publications

(1 citation statement)

References 83 publications

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“…A healthy gut contributes to the resilience of aquatic species by helping them cope with stress more effectively. Stress can compromise the integrity of the gut lining, leading to conditions like inflammation and increased susceptibility to diseases (McLaren and Callahan, 2020;Liu et al, 2023). Gut health is also linked to the immune system of aquatic species.…”

Section: Duplicated Genes and The Plastic Lifestyle Of The Gilthead S...mentioning

confidence: 99%

The Two Genomes of Gilthead Sea Bream (Sparus aurata): a Multi-Omics and Holobiont Approach

Naya Català

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Summaries and Glossary 1. General Introduction 1.1. Aquaculture 1.2. Omics 2. Aims, outline, and institutional support 2.1. Aims 2.2. Outline 2.3. Institutional support 3. Transcriptomic profiling of Gh/Igf system reveals a prompted tissuespecific differentiation and novel hypoxia responsive genes in gilthead sea bream Abstract 3.1. Introduction 3.2. Results 3.3. Discussion 3.4. Methods Supplementary Material 4. Targeting the Mild-Hypoxia Driving Force for Metabolic and Muscle Transcriptional Reprogramming of Gilthead Sea Bream (Sparus aurata) Juveniles Simple summary Abstract 4.1. Introduction 4.2. Material and Methods 4.3. Results 4.4. Discussion 4.5. Conclusions Supplementary material 5. Broodstock nutritional programming differentially affects the hepatic transcriptome and genome-wide DNA methylome of farmed gilthead sea bream (Sparus aurata) depending on genetic background Abstract 5.1. Background 5.2. Results 5.3. Discussion 5.4. Conclusions 5.5. Methods Supplementary material 11 6. Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream Abstract 6.1. Background 6.2. Results 6.3. Discussion 6.4. Conclusions 6.5. Methods Supplementary Material 7. Diet and Host Genetics Drive the Bacterial and Fungal Intestinal Metatranscriptome of Gilthead Sea Bream Abstract 7.1. Introduction 7.2. Results 7.3. Discussion 7.4. Materials and Methods Supplementary Material 8. Genetics and Nutrition Drive the Gut Microbiota Succession and Host-Transcriptome Interactions through the Gilthead Sea Bream (Sparus aurata) Production Cycle Simple summary Abstract 8.1. Introduction 8.2. Materials and Methods 8.3. Results 8.4. Discussion 8.5. Conclusions Supplementary Material 9. Can the genetic background modulate the effects of feed additives? Answers from gut microbiome and transcriptome interactions in farmed gilthead sea bream (Sparus aurata) Abstract 9.1. Introduction 9.2. Materials and methods 9.3. Results 9.4. Discussion 9.5. Conclusions Supplementary Material 10. General Discussion 11. Conclusions(▲) Figure 1.2. Ongoing world main challenges affecting aquaculture and the ultimate goals needed to achieve by the sector. Sustainability and biotechnological, automation, and nutritional technological innovations are postulated as the mechanisms for the accomplishment of these goals, as well as to lead the greening of the aquaculture sector. (▲) Figure 1.4. Simplified scheme of the Gh/Igf endocrine axis controlling growth in teleost fish. Multiple hormonal and nutritional factors may promote (+) the production and/or influence (+/-) the activity of IGF-1. Negative feedback (-) by Igf1 inhibits growth hormone secretion by the pituitary. (▲) Figure 1.9. Overview of the main questions addressed by A genomics, B transcriptomics, epigenomics, and C microbiomics, the omics used in this PhD thesis, in aquaculture settings.Harnessing the potential of omics integration approaches, as well as applying their outcomes again to the aquaculture systems, emerge as a k...

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Section: Duplicated Genes and The Plastic Lifestyle Of The Gilthead S...mentioning

confidence: 99%

The Two Genomes of Gilthead Sea Bream (Sparus aurata): a Multi-Omics and Holobiont Approach

Naya Català

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Hypoxia-inducible factor-1α promotes macrophage functional activities in protecting hypoxia-tolerant large yellow croaker (Larimichthys crocea) against Aeromonas hydrophila infection

Zhang,

Wang,

Gao

et al. 2024

Front. Immunol.

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The immune system requires a high energy expenditure to resist pathogen invasion. Macrophages undergo metabolic reprogramming to meet these energy requirements and immunologic activity and polarize to M1-type macrophages. Understanding the metabolic pathway switching in large yellow croaker (Larimichthys crocea) macrophages in response to lipopolysaccharide (LPS) stimulation and whether this switching affects immunity is helpful in explaining the stronger immunity of hypoxia-tolerant L. crocea. In this study, transcript levels of glycolytic pathway genes (Glut1 and Pdk1), mRNA levels or enzyme activities of glycolytic enzymes [hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), and lactate dehydrogenase A (LDHA)], aerobic respiratory enzymes [pyruvate dehydrogenase (PDH), isocitrate dehydrogenase (IDH), and succinate dehydrogenase (SDH)], metabolites [lactic acid (LA) and adenosine triphosphate (ATP)], levels of bactericidal products [reactive oxygen species (ROS) and nitric oxide (NO)], and transcripts and level changes of inflammatory factors [IL1β, TNFα, and interferon (IFN) γ] were detected in LPS-stimulated L. crocea head kidney macrophages. We showed that glycolysis was significantly induced, the tricarboxylic acid (TCA) cycle was inhibited, and metabolic reprogramming occurred, showing the Warburg effect when immune cells were activated. To determine the potential regulatory mechanism behind these changes, LcHIF-1α was detected and found to be significantly induced and transferred to the nucleus after LPS stimulation. LcHif-1α interference led to a significant reduction in glycolytic pathway gene transcript expression, enzyme activity, metabolites, bactericidal substances, and inflammatory factor levels; a significant increase in the aerobic respiration enzymes; and decreased migration, invasion, and phagocytosis. Further ultrastructural observation by electron microscopy showed that fewer microspheres contained phagocytes and that more cells were damaged after LcHif-1α interference. LcHif-1α overexpression L. crocea head kidney macrophages showed the opposite trend, and promoter activities of Ldha and Il1β were significantly enhanced after LcHif-1α overexpression in HEK293T cells. Our data showed that LcHIF-1α acted as a metabolic switch in L. crocea macrophages and was important in polarization. Hypoxia-tolerant L. crocea head kidney showed a stronger Warburg effect and inhibited the TCA cycle, higher metabolites, and bactericidal substance levels. These results collectively revealed that LcHif-1α may promote the functional activities of head kidney macrophages in protecting hypoxia-tolerant L. crocea from Aeromonas hydrophila infection.

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Gut microbial and metabolic characterization of Atlantic salmon (Salmon salar) challenged with Aeromonas salmonicida

Cited by 2 publications

References 83 publications

The Two Genomes of Gilthead Sea Bream (Sparus aurata): a Multi-Omics and Holobiont Approach

The Two Genomes of Gilthead Sea Bream (Sparus aurata): a Multi-Omics and Holobiont Approach

Hypoxia-inducible factor-1α promotes macrophage functional activities in protecting hypoxia-tolerant large yellow croaker (Larimichthys crocea) against Aeromonas hydrophila infection

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