Gut microbes exacerbate systemic inflammation and behavior disorders in neurologic disease CADASIL

Abstract

Background Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a cerebral small vessel disease that carries mutations in NOTCH3. The clinical manifestations are influenced by genetic and environmental factors that may include gut microbiome. Results We investigated the fecal metagenome, fecal metabolome, serum metabolome, neurotransmitters, and cytokines in a cohort of 24 CADASIL patients with 28 healthy household controls. The integrated-omics study showed CADASIL patients harbored an altered microbiota composition and functions. The abundance of bacterial coenzyme A, thiamin, and flavin-synthesizing pathways was depleted in patients. Neurotransmitter balance, represented by the glutamate/GABA (4-aminobutanoate) ratio, was disrupted in patients, which was consistent with the increased abundance of two major GABA-consuming bacteria, Megasphaera elsdenii and Eubacterium siraeum. Essential inflammatory cytokines were significantly elevated in patients, accompanied by an increased abundance of bacterial virulence gene homologs. The abundance of patient-enriched Fusobacterium varium positively correlated with the levels of IL-1β and IL-6. Random forest classification based on gut microbial species, serum cytokines, and neurotransmitters showed high predictivity for CADASIL with AUC = 0.89. Targeted culturomics and mechanisms study further showed that patient-derived F. varium infection caused systemic inflammation and behavior disorder in Notch3R170C/+ mice potentially via induction of caspase-8-dependent noncanonical inflammasome activation in macrophages. Conclusion These findings suggested the potential linkage among the brain-gut-microbe axis in CADASIL.

Gut pathobiome mediates behavioral and developmental disorders in biotoxin-exposed amphibians

“…Recently, a pioneering study shed light on the disruption of the normal metabolic rhythm of the gut microbiome in host mammals with a high-fat diet, indicating a potential link between the gut pathobiome and diseases caused by extrinsic factors [ 40 ]. A study suggested that the gut pathobiome could exacerbate systemic inflammation and behavioral disorders in the neurological disease CADASIL [ 41 ]. However, the causality between the gut pathobiome and host diseases, especially upon exposure to emerging biotoxin pollution, remained largely unexplored and elusive.…”

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