Gut Metagenome as a Potential Diagnostic and Predictive Biomarker in Slow Transit Constipation

Tian, Hui; Wang, Hongzhi; Yang, Bo; Cui, Jiaqu; Zheng, Zhijun; Wu, Chunyan; Zhou, Shen; Lv, Xiaoqiong; Qin, Nan; Qin, Huanlong; Li, Ning; Chen, Qiyi

doi:10.3389/fmed.2021.777961

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…However, in our study, their decrease in FC patients suggests that a certain abundance of Escherichia–Shigella and Klebsiella may benefit the balance of the intestinal microenvironment. Alistipes and Parabacteroides have been reported to increase in patients with slow transit constipation (STC), while Klebsiella is enriched in normal controls [ 24 ]. Such changes are consistent with our findings.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Wang

et al. 2023

Nutrients

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Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the relationship between the gut microbiome and metabolites. This study used 16S rRNA microbial genomics and non-target metabolome based on liquid chromatography–mass spectrometry to analyze the gut microbiota composition and serum metabolic profiles of 30 FC patients and 28 healthy individuals. We found that patients with FC and healthy individuals have different gut microbiota structures and serum metabolic profiles. FC patients had more Bacteroides and butyrate-producing bacteria (Roseburia, Faecaliberium, Butyriccoccus). The upstream products of host arginine biosynthesis (2-oxoglutaric acid, L-glutamic acid, N-acetylornithine, and L-ornithine) were significantly reduced in FC patients’ serum metabolites. In summary, our study describes the gut microbiome and serum metabolome of patients with functional constipation. It reveals that functional constipation may be associated with increased Bacteroidetes and downregulation of upstream products of host arginine biosynthesis, which may be potential markers for diagnosing functional constipation.

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Section: Discussionmentioning

confidence: 99%

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Wang

et al. 2023

Nutrients

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“…All of the above were flora involved in the allergic immune response of the organism, suggesting that the emergence and progress of PUD may be strongly involved in the ecological immunity of the gastrointestinal microenvironment. Sequencing studies of slow transit constipation patients and colorectal cancer patients both identified significant enrichment of Alistipes and suggested that it could be used as a potential pathogenic genus and diagnostic marker for gastrointestinal diseases ( 45 , 46 ). Coprococcus_2 was significantly enriched as a probiotic in HC patients, and a clinical study of irritable bowel syndrome ( 47 ) reached similar conclusions.…”

Section: Discussionmentioning

confidence: 99%

Integrating microbiome and metabolome revealed microbe-metabolism interactions in the stomach of patients with different severity of peptic ulcer disease

Wang

Xiao²,

Lin³

et al. 2023

Front. Immunol.

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BackgroundPeptic ulcer disease (PUD) is a multi-cause illness with an unknown role for gastric flora and metabolism in its pathogenesis. In order to further understand the pathogenesis of gastric flora and metabolism in PUD, this study used histological techniques to analyze the microbiome and metabolome of gastric biopsy tissue. In this paper, our work described the complex interactions of phenotype-microbial-metabolite-metabolic pathways in PUD patients at different pathological stages.MethodsGastric biopsy tissue samples from 32 patients with chronic non-atrophic gastritis, 24 patients with mucosal erosions, and 8 patients with ulcers were collected for the microbiome. UPLC-MS metabolomics was also used to detect gastric tissue samples. These datasets were analyzed individually and integrated using various bioinformatics methods.ResultsOur work found reduced diversity of gastric flora in patients with PUD. PUD patients at different pathological stages presented their own unique flora, and there were significant differences in flora phenotypes. Coprococcus_2, Phenylobacterium, Candidatus_Hepatoplasma, and other bacteria were found in the flora of people with chronic non-atrophic gastritis (HC). The representative flora of mucosal erosion (ME) had uncultured_bacterium_c_Subgroup_6, Sphingomonadaceae, Xanthobacteraceae, and uncultured_bacterium_f_Xanthobacteraceae. In comparison, the characteristic flora of the PUD group was the most numerous and complex, including Ruminococcus_2, Agathobacter, Alistipes, Helicobacter, Bacteroides and Faecalibacterium. Metabolomics identified and annotated 66 differential metabolites and 12 significantly different metabolic pathways. The comprehensive analysis correlated microorganisms with metabolites at different pathological stages and initially explored the complex interactions of phenotype-microbial-metabolite-metabolic pathways in PUD patients at different pathological stages.ConclusionOur research results provided substantial evidence to support some data on the analysis of the microbial community and its metabolism in the stomach, and they demonstrated many specific interactions between the gastric microbiome and the metabolome. Our study can help reveal the pathogenesis of PUD and indicate plausible disease-specific mechanisms for future studies from a new perspective.

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“…g_Pseudoramibacter-Eubacterium, the primary producer of short-chain fatty acids, can provide energy for intestinal cells and act as an anti-inflammatory factor in the intestinal tract by metabolizing dietary fiber [ 51 ]. However, studies [ 52 ] have shown that Eubacter has a high abundance in patients with constipation, which requires further study in the future.…”

Section: Discussionmentioning

confidence: 99%

Association between Depression, Anxiety Symptoms and Gut Microbiota in Chinese Elderly with Functional Constipation

Liang

Zhao²,

et al. 2022

Nutrients

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This study aimed to investigate the relationship between anxiety, depression, and gut microbiota in elderly patients with FC. Methods: in this cross-sectional study, a total of 198 elderly participants (85 male and 113 female) aged over 60 years were recruited. The study was conducted in Changsha city, China. The participants completed an online questionnaire, including The Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 (GAD-7), The Patient Assessment of Symptoms (PAC-SYM), and The Patient Assessment of Quality of Life (PAC-QoL). We selected the 16S rDNA V3 + V4 region as the amplification region and sequenced the gut microbiota using the Illumina Novaseq PE250 high-throughput sequencing platform. Results: in total, 30.3% of patients with constipation had depression, while 21.3% had anxiety. The relative abundance of intestinal microbiota in the normal group was higher than that in the anxiety and depression group. According to LEfSe analysis, the relative abundance of g_Peptoniphilus and g_Geobacter in the people without depression and anxiety was higher. The relative abundance of g_Pseudoramibacter-Eubacterium and g_Candidatus-Solibacter in the depression group was lower, and the relative abundance of g_Bacteroides and g_Paraprevotella, g_Cc_115 in the anxiety group was higher. In addition, according to the correlation analysis, g_Aquicella and g_Limnohabitans were negatively correlated with constipation symptoms, anxiety, and depression. Conclusions: this study found that gut microbiota composition may be associated with a higher incidence of anxiety and depression in patients with FC, thus providing insight into the mechanisms that ameliorate mood disorders in patients with FC.

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Gut Metagenome as a Potential Diagnostic and Predictive Biomarker in Slow Transit Constipation

Cited by 13 publications

References 40 publications

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Integrating microbiome and metabolome revealed microbe-metabolism interactions in the stomach of patients with different severity of peptic ulcer disease

Association between Depression, Anxiety Symptoms and Gut Microbiota in Chinese Elderly with Functional Constipation

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