Gut colonization with extended-spectrum β-lactamase-producing Enterobacteriaceae may increase disease activity in biologic-naive outpatients with ulcerative colitis: an interim analysis

Skuja, Vita; Derovs, Aleksejs; Pekarska, Katrina; Rudzite, Dace; Lavrinovica, Elvira; Piekuse, Linda; Kempa, Inga; Straume, Zane; Eglīte, Jeļena; Lejnieks, Aivars; Krūmiņa, Angelika; Eliakim, Rami

doi:10.1097/meg.0000000000000989

Cited by 10 publications

(6 citation statements)

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“…Enterobacteriaceae and Desulfovibrionaceae were abundant in mice with acute colitis, while Rikenellaceae , Akkermansiaceae and Prevotellaceae showed lower abundance. Excessive proliferation of Enterobacteriaceae has been observed in the gut of UC patients [ 32 , 33 ]. Surprisingly, the SCFAs-producing Rikenellaceae , Akkermansiaceae and Prevotellaceae were raised in mice with chronic colitis, which is contradictory to existing reports.…”

Section: Discussionmentioning

confidence: 99%

Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile

Huang

Liu

et al. 2021

BMC Microbiol

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Background Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic colitis. Methods Acute colitis was induced in mice by administering 3% DSS for 1 week in the drinking water, and chronic colitis was induced by supplementing drinking water with 2.5% DSS every other week for 5 weeks. Control groups received the same drinking water without DSS supplementation. The histopathological score and length of the colons, and disease activity index (DAI) were evaluated to confirm the presence of experimental colitis. Intestinal microbiota was profiled by 16S rDNA sequencing of cecal content. Results Mice with both acute and chronic DSS-triggered colitis had significantly higher DAI and colon histopathological scores in contrast to the control groups (P < 0.0001, P < 0.0001), and the colon was remarkably shortened (P < 0.0001, P < 0.0001). The gut microbiota α-diversity was partly downregulated in both acute and chronic colitis groups in contrast to their respective control groups (Pielou index P = 0.0022, P = 0.0649; Shannon index P = 0.0022, P = 0.0931). The reduction in the Pielou and Shannon indices were more obvious in mice with acute colitis (P = 0.0022, P = 0.0043). The relative abundance of Bacteroides and Turicibacter was increased (all P < 0.05), while that of Lachnospiraceae, Ruminococcaceae, Ruminiclostridium, Rikenella, Alistipes, Alloprevotella, and Butyricicoccus was significantly decreased after acute DSS induction (all P < 0.05). The relative abundance of Bacteroides, Akkermansia, Helicobacter, Parabacteroides, Erysipelatoclostridium, Turicibacter and Romboutsia was also markedly increased (all P < 0.05), and that of Lachnospiraceae_NK4A136_group, Alistipes, Enterorhabdus, Prevotellaceae_UCG-001, Butyricicoccus, Ruminiclostridium_6, Muribaculum, Ruminococcaceae_NK4A214_group, Family_XIII_UCG-001 and Flavonifractor was significantly decreased after chronic DSS induction (all P < 0.05). Conclusion DSS-induced acute and chronic colitis demonstrated similar symptoms and histopathological changes. The changes in the gut microbiota of the acute colitis model were closer to that observed in UC. The acute colitis model had greater abundance of SCFAs-producing bacteria and lower α-diversity compared to the chronic colitis model.

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Section: Discussionmentioning

confidence: 99%

Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile

Huang

Liu

et al. 2021

BMC Microbiol

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“…Previous studies showed that patients with IBD had increased abundances of Enterobacteriaceae, including one study reporting Enterobacteriaceae as a dysbiotic signature in patients with IBD in clinical remission. 26,27 In a microbiome study of patients with irritable bowel syndrome (IBS), an increased abundance of Enterobacteriaceae was observed compared to that in the healthy controls. 28 The pathogenicity of Enterobacteriaceae spp.…”

Section: Discussionmentioning

confidence: 99%

“…Enterobacteriaceae_g were enriched in the altered mucosa group compared to those in the preserved mucosa or control groups and were also found to be a marker of symptomatic non‐remission group. Previous studies showed that patients with IBD had increased abundances of Enterobacteriaceae , including one study reporting Enterobacteriaceae as a dysbiotic signature in patients with IBD in clinical remission 26,27 . In a microbiome study of patients with irritable bowel syndrome (IBS), an increased abundance of Enterobacteriaceae was observed compared to that in the healthy controls 28 .…”

Section: Discussionmentioning

confidence: 99%

Gut microbial signatures in clinically stable ulcerative colitis according to the mucosal state and associated symptoms

Kim,

Jung,

Lee

et al. 2023

J of Gastro and Hepatol

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Background and AimThe gut microbiome of patients with clinically stable ulcerative colitis (UC) differs from that of healthy individuals depending on the state of the colonic mucosa, especially with or without advanced scarring; however, the underlying mechanism is unclear. Therefore, this study examined the gut microbiome compositional signatures in patients with significant mucosal scarring and UC‐related symptoms.MethodsStool samples for gut microbiome analysis were prospectively collected from 57 patients with clinically stable UC between January 1 and December 31, 2022. Data from 57 individuals without inflammatory bowel disease (non‐IBD) paired by age and sex were selected from our previous study as the control group. The fecal samples were subjected to 16S rRNA gene sequencing. Associations between gut microbiome profiles and clinical or colonoscopic assessments were examined using diversity and differential abundance analyses.ResultsGut microbiome compositions between the patients with clinically stable UC and non‐IBD controls differed significantly. Furthermore, gut microbiome compositions varied between the preserved and altered mucosa groups identified based on mucosal changes in the UC group. Differential abundance test of patients with UC for symptomatic remission based on stool frequency from the two‐item patient‐reported outcome identified several overlapping taxa specified as gut microbiome signatures, including the Enterobacteriaceae unknown genera (Enterobacteriaceae_g), Klebsiella, and several Lachnospiraceae spp. both in mucosal and symptom change analyses.ConclusionsThe gut microbiome can change with mucosal changes, even in clinically stable UC, and some gut microbial signatures may explain the symptom manifestations in patients with UC showing significant mucosal changes.

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“…The improved detection of pathogens has two goals: (1) improving poultry health which safeguards the food supply; and (2) reducing the transmission of antibiotic resistant pathogens and elements. There is a rise in foodborne pathogens containing antibiotic resistance elements that are capable of disseminating to the naïve microbiota ( Nguyen et al, 2016 ; Kassem et al, 2017 ; Vita et al, 2018 ). Therefore, the uncontrolled spread of antibiotic resistant foodborne pathogens increases the risk to the food supply and potentially result in resistant clinical infections ( Economou and Gousia, 2015 ; McCrackin et al, 2015 ; Battersby et al, 2017 ; Kassem et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR

et al. 2018

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Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathogens within a flock. The surface contamination of production eggs with invasive pathogens such as Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes during embryogenesis results in gastrointestinal tract (GIT) colonization. Pathogens that secure a niche within the GIT during embryonic development are nearly impossible to eradicate from the food chain. Therefore, current monitoring paradigms are not comprehensive because they fail to capture the presence of invasive pathogens within the embryonic GIT rapidly. By developing tools to recognize the pathogens’ presence in the GIT during embryogenesis, producers are then able to spot evaluate broiler eggs for their potential risk as carriers of foodborne pathogens. In this study a novel qPCR assay was developed to semi-quantify pathogen load relative to total bacterial burden. Eggs sampled from three independent production broiler flocks of different ages were assayed for S. enterica (invA), C. jejuni (HipO), and L. monocytogenes (HlyA) against total microbial load (16s). The eggs were sampled at 1-day post-set within each flock, 2 weeks post-set, after vaccination (at 2.5 weeks) and 1-day post-hatch. The eggs were washed, and the yolk and embryonic chick GIT were collected. The DNA was extracted and subjected to a qPCR assay. The results confirm a novel technique for pathogen monitoring relative to total bacterial load and a unique method for monitoring the dynamics of foodborne pathogen invasion throughout broiler egg production.

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Gut colonization with extended-spectrum β-lactamase-producing Enterobacteriaceae may increase disease activity in biologic-naive outpatients with ulcerative colitis: an interim analysis

Abstract: Gut colonization with ESBL-producing Enterobacteriaceae may increase UC disease activity. Further research is needed to analyze the possible confounding factors that could contribute toward this outcome.

Cited by 10 publications

References 63 publications

Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile

Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile

Gut microbial signatures in clinically stable ulcerative colitis according to the mucosal state and associated symptoms

Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR

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