Gut Bacterial Communities in Geographically Distant Populations of Farmed Sea Bream (Sparus aurata) and Sea Bass (Dicentrarchus labrax)

Nikouli, Eleni; Meziti, Alexandra; Antonopoulou, Efthimia; Mente, Eleni; Kormas, Konstantinos Ar.

doi:10.3390/microorganisms6030092

Cited by 47 publications

(55 citation statements)

References 63 publications

(69 reference statements)

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“…The remaining 12 genera were previously reported in the gut microflora of freshwater fishes such as Ctenopharyngodon idellus [18], Fundulus heteroclitus [19], Oncorhynchus mykiss [20,21], Danio rerio [22], Carassius auratus [23], Carassius gibelio [24], and Coregonus baicalensis [25]. With the rapid development of modern molecular biotechnology, an increasing number of novel microbial communities and functions have recently been detected in the guts of fishes by using new techniques and methods [7][8][9]. Our results suggested that carnivorous coral reef fishes from the South China Sea harbor many previously unknown components of the gut microbial community.…”

Section: New Insights Into the Gut Microbial Communities Of Coral Reementioning

confidence: 99%

“…The fish-associated symbiotic gut microbiota play a crucial role in nutritional provision and metabolic homeostasis. Nikouli et al found that the gut microbial communities of farmed sea bream (Sparus aurata) fed different diets could influence the host's nutritional intake [7]. Another study reported that the intestinal microbiota of grass carp (Ctenopharyngodon idellus) fed with faba beans could significantly enhance the host's metabolic functions [8].…”

Section: Introductionmentioning

confidence: 99%

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Deep Insights into Gut Microbiota in Four Carnivorous Coral Reef Fishes from the South China Sea

et al. 2020

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Investigations of gut microbial diversity among fish to provide baseline data for wild marine fish, especially the carnivorous coral reef fishes of the South China Sea, are lacking. The present study investigated the gut microbiota of four carnivorous coral reef fishes, including Oxycheilinus unifasciatus, Cephalopholis urodeta, Lutjanus kasmira, and Gnathodentex aurolineatus, from the South China Sea for the first time using high-throughput Illumina sequencing. Proteobacteria, Firmicutes, and Bacteroidetes constituted 98% of the gut microbiota of the four fishes, and 20 of the gut microbial genera recovered in this study represent new reports from marine fishes. Comparative analysis indicated that the four fishes shared a similar microbial community, suggesting that diet type (carnivorous) might play a more important role in shaping the gut microbiota of coral reef fishes than the species of fish. Furthermore, the genera Psychrobacter, Escherichia-Shigella, and Vibrio constituted the core microbial community of the four fishes, accounting for 61-91% of the total sequences in each fish. The lack of the genus Epulopiscium in the four fishes was in sharp contrast to what has been found in coral reef fishes from the Red Sea, in which Epulopiscium was shown to be the most dominant gut microbial genus in seven herbivorous coral reef fishes. In addition, while unique gut microbial genera accounted for a small proportion (8-13%) of the total sequences, many such genera were distributed in each coral reef fish species, including several genera (Endozoicomonas, Clostridium, and Staphylococcus) that are frequently found in marine fishes and 11 new reports of gut microbes in marine fishes. The present study expands our knowledge of the diversity and specificity of gut microbes associated with coral reef fishes.

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Section: New Insights Into the Gut Microbial Communities Of Coral Reementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Deep Insights into Gut Microbiota in Four Carnivorous Coral Reef Fishes from the South China Sea

et al. 2020

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“…Mollicutes are an interesting group of bacteria, lacking a cell wall, and include Mycoplasmas which are well-known fish parasites [94]. Some studies have proposed that Mycoplasmas are commensal gut or even intra-host dependent microorganisms in Atlantic salmon [29], [95]. Gemmatimonadetes are largely uncharacterised though have been found previously in the gut of sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax), at low abundances [96].…”

Section: Discussionmentioning

confidence: 99%

Temporal changes in the gut microbiota in farmed Atlantic cod (Gadus morhua) outweigh the response to diet supplementation with macroalgae

Keating

Bolton‐Warberg

Hinchcliffe

et al. 2020

Preprint

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Background: Aquaculture successfully meets global food demands for many fish species. However, squaculture production of Atlantic cod (Gadus morhua) is modest in comparison to market demand. For cod farming to be a viable economic venture specific challenges on how to increase growth, health and farming productivity need to be addressed. Feed ingredients play a key role here. Macroalgae (seaweeds) have been suggested as a functional feed supplement with both health and economic benefits for terrestrial farmed animals and fish. The impact of such dietary supplements to cod gut integrity and microbiota, which contribute to overall fish robustness is unknown. The objective of this study was to supplement the diet of juvenile Atlantic cod with macroalgae and determine the impacts on fish condition and growth, gut morphology and hindgut microbiota composition (16S rRNA amplicon sequencing). Fish were fed one of three diets: control (no macroalgal inclusion), 10% inclusion of either egg wrack (Ascophyllum nodosum) or sea lettuce (Ulva rigida) macroalgae in a 12-week trial. Results: The results demonstrated that there was no significant difference in fish condition, gut morphology or hindgut microbiota between the U. rigida supplemented fish group and the control group at any time point. This contrasts with the A. nodosum treatment. Fish within this group were further categorised as either 'Normal' or 'Lower Growth'. 'Lower Growth' individuals found the diet unpalatable resulting in reduced weight and condition factor combined with an altered gut morphology and microbiome relative to the other treatments. Excluding this group, our results show that the hindgut microbiota were largely driven by temporal pressures with the microbial communities becoming more similar over time irrespective of dietary treatment. The core microbiome at the final time-point consisted of the orders Vibrionales (Vibrio and Photobacterium), Bacetroidales (Bacetroidetes and Macellibacteroides) and Clostridiales (Lachnoclostridium. Conclusions: Our study indicates that U. rigida macroalgae can be supplemented at 10% inclusion levels in the diet of jevenile farmed Atlantic cod without any impact on fish condition or hindgut microbial community structure. We also conclude that 10% dietary inclusion of A. nodosum is not a suitable feed supplement in a farmed cod diet.

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“…Due to its economic value, many studies on gilthead sea bream intestinal microbiota have been conducted. These studies were mainly focused on de ning baseline populations [35][36][37] or changes induced by diet [18,[38][39][40][41][42][43][44] or environmental conditions [45]. The microbiota is composed of very dynamic populations that are affected by different factors [18,36,[53][54][55]42,[46][47][48][49][50][51][52] such as diet, season, habitat, rearing density, age, sex and genetic background, the focus of the current study.…”

Section: Introductionmentioning

confidence: 99%

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Piazzon

Naya-Català

Perera

et al. 2020

Preprint

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Background The key effects of intestinal microbiota in animal health has led to an increasing interest in manipulating these bacterial populations to improve animal welfare. The aquaculture sector is no exception and in the last years many studies have described these populations in different fish species. However, this is not an easy task, as intestinal microbiota is composed of very dynamic populations that are influenced by different factors, such as diet, environment, host age and genetics. In the current study, we aimed to determine whether the genetic background of gilthead sea bream ( Sparus aurata ) influences the intestinal microbial composition, how these bacterial populations are modulated by dietary changes, and the effect of selection by growth on intestinal disease resistance. To that aim, three different groups of five families of gilthead sea bream that were selected during two generations for fast, intermediate or slow growth (F3 generation) were kept together in the same open-flow tanks and fed a control or a well-balanced plant-based diet during nine months. Six animals per family and dietary treatment were sacrificed and the adherent bacteria from the anterior intestinal portion were sequenced. In parallel, fish of the fast- and slow-growth groups were infected with the intestinal parasite Enteromyxum leei and the disease signs, prevalence, intensity and parasite abundance were evaluated. Results No differences were detected in alpha diversity indexes among families, and the core bacterial architecture was the prototypical composition of gilthead sea bream intestinal microbiota, indicating no dysbiosis in any of the groups. The plant-based diet significantly changed the microbiota in the intermediate- and slow-growth families, with a much lower effect on the fast-growth group. Interestingly, the smaller changes detected in the fast-growth families potentially accounted for more changes at the metabolic level when compared to the other families. Upon parasitic infection, the fast-growth group showed significantly lower disease signs and parasite intensity and abundance than the slow-growth animals. Conclusions These results show a clear genome-metagenome interaction indicating that the fast-growth families harbor a microbiota that is more flexible upon dietary changes. These animals also showed a better ability to cope with intestinal infections.

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Gut Bacterial Communities in Geographically Distant Populations of Farmed Sea Bream (Sparus aurata) and Sea Bass (Dicentrarchus labrax)

Cited by 47 publications

References 63 publications

Deep Insights into Gut Microbiota in Four Carnivorous Coral Reef Fishes from the South China Sea

Deep Insights into Gut Microbiota in Four Carnivorous Coral Reef Fishes from the South China Sea

Temporal changes in the gut microbiota in farmed Atlantic cod (Gadus morhua) outweigh the response to diet supplementation with macroalgae

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

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