Gut bacteria, bacteriophages, and probiotics: Tripartite mutualism to quench the SARS-CoV2 storm

Zeinali, Tahereh; Faraji, Niloofar; Joukar, Farahnaz; Mirzaei, Mohammadali Khan; Jalali, H.; Shenagari, Mohammad; Ghanaei, F Mansour

doi:10.1016/j.micpath.2022.105704

Cited by 7 publications

(4 citation statements)

References 152 publications

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“…Viral, bacterial and parasite antigens have been produced in L. lactis. Additionally, IL-12 (IL-12, a cytokine aid in fighting against several bacterial, viral and parasite infections) has been made using L. lactis (Zeinali et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Viral, bacterial and parasite antigens have been produced in L. lactis . Additionally, IL‐12 (IL‐12, a cytokine aid in fighting against several bacterial, viral and parasite infections) has been made using L. lactis (Zeinali et al., 2022 ). Additionally, the treatment of L. lactis isolates secreting IL‐12 and L. lactis isolates displaying cell wall–anchored HPV‐16 E7 proteins significantly improved the immune reaction following co‐administration (Bermúdez‐Humarán et al., 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

Gouran,

Doosti,

Jami

2023

Veterinary Medicine & Sci

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Background Most Brucella infections take place on mucosal membranes. Therefore, creating vaccinations delivered through the mucosa may be crucial for managing brucellosis. Consequently, we assessed the efficacy of a recombinant oral antigen delivery system based on Lactococcus lactis for Brucella abortus omp25 antigen. Method Oral vaccinations with L. lactis transformed with pNZ8148 variants encoding for omp25 (pNZ8148:omp25) and free‐pNZ8148 were administered to mice. On day 30, following immunization in animal groups, anti‐omp25‐specific IgG1 antibodies were assessed by the ELISA test. Additionally, nasal and bronchoalveolar lavages containing omp25‐specific secretory IgA (sIgA) were analysed by ELISA. ELISA test and real‐time PCR were also used to analyse cytokine responses up to 28 days following the last boost. In addition, the protective potential of L. lactis pNZ8148:omp25 vaccines was assessed in BALB/c mice by exposing them to the B. abortus strain. Results Based on the initial screening results, the omp25 protein was identified for immunogenicity because it had the maximum solubility and flexibility and antigenic values of 0.75. The produced plasmid was digested using KpnI and XbaI. By electrophoretic isolation of the digestion fragments at 786 bp, the omp25 gene, the successful production of the recombinant plasmid, was confirmed. Antigen expression at the protein level revealed that the target group generated the 25 kDa‐sized omp25 protein, but there was no protein expression in the control group. Fourteen days after priming, there was a considerable amount of omp25‐specific IgG1 in the sera of mice vaccinated with pNZ8148–Usp45–omp25–L. lactis (p < 0.001 in target groups compared to the phosphate‐buffered saline control group). IFN‐γ and TNF‐α levels were more significant in samples from mice that had been given the pNZ8148–Usp45–omp25–L. lactis and IRBA vaccinations, in samples taken on days 14 and 28, respectively (p < 0.001). The pNZ8148–Usp45–omp25–L. lactis and IRBA immunization groups had significantly greater IL‐4 and IL‐10 transcription levels than the other groups. The spleen portions from the pNZ8148–Usp45–omp25–L. lactis and IRIBA vac group had less extensive spleen injuries, alveolar oedema, lymphocyte infiltration and morphological damage due to the inflammatory process. Conclusion Our study offers a novel method for using the food‐grade, non‐pathogenic and noncommercial bacterium L. lactis as a protein cell factory to produce the novel immunogenic fusion candidate romp25. This method offers an appealing new approach to assessing the cost‐effective, safe, sustainable, simple pilot development of pharmaceutical products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

Gouran,

Doosti,

Jami

2023

Veterinary Medicine & Sci

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“…The most important receptor in human host cells is the angiotensin-converting enzyme 2 (ACE2) receptor that provides the entry for SARS-CoV-2 [3]. Both immune impairment and excessive immune response to SARS-CoV-2 result in severe conditions in patients [4].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Smoking and Opioid Consumption on the Severity of the Disease and Duration of Hospitalization in COVID-19 Patients

Fayazi¹,

Naeimi²,

Yaseri³

et al. 2023

Acta Medica Bulgarica

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Objective In the Coronavirus 2019 (COVID-19) global pandemic, various studies on the target communities of this virus were widely started and rapidly progressed. Smokers and opioid consumers are one of the virus targets since they have a vulnerable respiratory system. Due to the contradictory results in previous studies and the lack of similar investigations in this area, we aimed to perform this study to investigate the effect of smoking and opioid consumption on the consequences of the COVID-19 disease. Materials and Methods In this retrospective study, the required information was collected and analyzed from the archives of Razi Hospital, Rasht, Iran. Study variables included age, sex, the need for intubation, hospital length of stay, history of current smoking or opioid consumption, and intensive care unit (ICU) admission, ICU length of stay, admission oxygen saturation, disease severity, and the outcome of death or recovery. Data were collected and divided into the case (including current cigarette smokers, opioid consumers, and cigarette-opioid consumers) and control (non-smokers and non-opioid-consumers) groups. Out of 986 patients, 489 patients met the criteria for inclusion and subsequent analysis. The average age was 69.79 ± 16.06, and 294 (60.1%) patients were male. The median age of the case group (65.15 ± 42.41) was older than the control group (57.45 ± 15.71, P = 0.001). The case group consisted of more male patients than the control group (P = 0.001). Results The adjusted regression models demonstrated that current cigarette smoking, opioid, and cigarette-opioid consumption did not significantly predict hospital and ICU length of stay, ICU admission, disease severity, and mortality outcomes (P > 0.05). Current cigarette smoking and opioid consumption could not be an independent predictor for the consequences of ICU admission, hospital and ICU length of stay, the need for intubation, disease severity, and mortality in COVID-19 patients.

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“…Since the evaluation of clinical and laboratory findings are more accessible and valuable for the diagnosis of infections such as SARS-CoV-2 [14]; we aimed to evaluate demographical data, clinical characteristics, and laboratory findings of patients with COVID-19 in the North of Iran.…”

Section: Introductionmentioning

confidence: 99%

Clinical characteristics and laboratory findings of patients with COVID-19 in Rasht, Iran

et al. 2022

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In December 2019, a pandemic of an unknown respiratory virus emerged in Wuhan, China, putting the whole world in crisis; a newly emerged coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, we evaluated the clinical characteristics and laboratory findings of patients with coronavirus disease 2019 (COVID-19) in the North of Iran. Demographical data and clinical characteristics of 126 patients with COVID-19 in Razi Hospital, Rasht, Iran, were recorded. The mean age of patients was 62 years old and 57.1% of cases were male. About 17.5% of patients had direct contact with a SARS-CoV-2-infected patient. The most common underlying diseases were lung diseases (11.9%), diabetes (11.9%), cardiovascular disease (CVD) (7.1%), and hypertension (4.8%). The mean levels of lactate dehydrogenase (LDH), creatine phosphokinase (CPK), creatine kinase MB (CK-MB), serum glutamic oxaloacetic transaminase (SGOT), and erythrocyte sedimentation rate (ESR) (1231.

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Gut bacteria, bacteriophages, and probiotics: Tripartite mutualism to quench the SARS-CoV2 storm

Cited by 7 publications

References 152 publications

Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

The Effect of Smoking and Opioid Consumption on the Severity of the Disease and Duration of Hospitalization in COVID-19 Patients

Clinical characteristics and laboratory findings of patients with COVID-19 in Rasht, Iran

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