Gustatory response in the shimaisaki (tigerfish) Therapon oxyrhynchus.

Hidaka, Iwao; Ishida, Yusuke

doi:10.2331/suisan.51.387

Cited by 16 publications

(16 citation statements)

References 9 publications

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“…Nucleotide-sensitive fibers Although nucleotides are known gustatory stimulants to teleosts (Harada 1986;Hidaka and Ishida 1985;Ishida and Hidaka 1987;Kiyohara et al 1975), the finding that the tryptophan-sensitive group of fibers (28 of 83 fibers analyzed) in the yellowtail was also highly responsive to UMP (Hidaka and Zeng 1989) is contrary to what was found for the majority of amino acid-sensitive fibers in the present report for the channel catfish and for the Japanese puffer (Kiyohara et al 1975 ). For the channel catfish (Fig.…”

Section: Dose-responsecontrasting

confidence: 92%

Responses of single facial taste fibers in the channel catfish, Ictalurus punctatus, to amino acids

Kohbara

Michel²,

Caprio³

1992

Journal of Neurophysiology

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SUMMARYAND CONCLUSIONS 1. Amino acids and nucleotides stimulate taste receptors of teleosts. In this report, responses to these compounds of 105 facial taste fibers (79 fully characterized) that innervate maxillary barbel taste buds of the channel catfish (Ictalurus punctatus) were analyzed.2. The fully characterized facial taste fibers that responded to amino acids ( y2 = 68) were generally poorly responsive to nucleotides and related substances (NRS), whereas the fibers responsive to NRS (~2 = 11) were poorly responsive to amino acids. Spike discharge of the amino acid-responsive fibers to the most potent amino acid stimulus tested per fiber increased 44-fold from a mean spontaneous activity of 2.1 t 3.5 to 92.1 t 42.4 (SD) spikes/3 s. Spike activity of the NRS-responsive fibers to NRS increased 11 S-fold from a mean spontaneous activity of 3.4 t 5.9 to 39.1 t 27.4 spikes/3 s. There was no significant difference between the spontaneous rates, but stimulus evoked spike rates for the amino acid-responsive fibers were significantly greater (P < 0.05; Mann-Whitney test) than those for the NRS-responsive fibers.3. Hierarchical cluster analysis based on the 3-s response time identified three major groups of neurons. The identified clusters comprised neurons that were highly responsive to either L-alanine (i.e., Ala cluster; y1 = 39) L-arginine (i.e., Arg cluster; yt = 29), or NRS (NRS cluster; y1 = 11) . Fibers comprising the Arg cluster were more narrowly tuned than those within the Ala cluster. This report further characterizes the responses to amino acids of the individual facial taste fibers comprising the Ala and Arg clusters.4. Subclusters were evident within both of the amino acid-responsive clusters. The Arg cluster was divisible into two subclusters dependent on the response to 1 mM L-proline. Twelve neurons that were significantly (P < 0.05; Mann-Whitney test) more responsive to L-proline than the remaining 17 neurons within the Arg cluster formed the Arg/ Pro subcluster; these latter 17 neurons comprised the Arg subcluster. However, there was no significant difference (Mann-Whitney test) in the response to L-arginine between fibers within either subcluster across four different response times analyzed. Fibers within the Ala cluster were generally poorly responsive to L-proline. Four alanine subclusters were suggested on the basis of their relative responses to L-alanine, D-alanine, L-arginine, and the NRS; however of the 39 fibers comprising the alanine cluster, two alanine subclusters comprised only two fibers each, and the third subcluster consisted of four fibers. Neuron classification into clusters and subclusters was little influenced by the four different response times analyzed.5. Single-fiber dose-response relationships indicate that the Lproline gustatory receptor is of relatively low affinity in comparison to the receptors for L-alanine and L-arginine. Electrophysiological thresholds of single fibers within the Ala and Arg clusters were approximately nanomolar for L-alanine and L-arginine, respe...

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Section: Dose-responsecontrasting

confidence: 92%

Responses of single facial taste fibers in the channel catfish, Ictalurus punctatus, to amino acids

Kohbara

Michel²,

Caprio³

1992

Journal of Neurophysiology

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“…A cyprinid, Pseudorasbora parva, shows similar response characteristics to that of channel catfish (Kiyohara et al, 1981). A herbivore (Tilapia zillii, Cichlidae) and seven marine species respond to all three types of amino acids (Goh and Tamura, 1980;Hidaka and Ishida, 1985;Ishida and Hidaka, 1987;Johnsen et al, 1990).…”

Section: Amino Acidsmentioning

confidence: 97%

“…Four marine species in this group are essentially of salmonid type, responding to L-Pro, L-Ala, and betaine Hidaka and Ishida, 1985;Ishida and Hidaka, 1987). However, in the puffer, receptors for L-Pro and L-Ala are separate from each other (Kiyohara and Hidaka, 1991).…”

Section: Amino Acidsmentioning

confidence: 99%

“…Fish species which have been shown to have gustatory sensitivities to nucleotides include: puffer ; minnow ( Pseudorasbora parva, Cyprinidae) (Kaku et al, 1980); yellowtail Zeng and Hidaka, 1990); tigerfish (Therapon oxyrhynchus, Theraponidae) (Hidaka and Ishida, 1985;Ishida and Hidaka, 1987); amberjack (Seriola dumerilli, Carangidae), jack mackerel (Trachurusjaponicus, Caran~-dae), club mackerel (Scomber japonicus, Scombridae), and grunt (Parapristipoma trilineatum, Haemulidae) (Ishida and Hidaka, 1987). All the effective nucleotides are of 5'-nucleotide type, and include adenosine-5'-monophosphate (Y-AMP), adenosine-5'-diphosphate (5'-ADP), adenosine-5'-triphosphate (5'-ATP), citidine-5'-monophosphate (5'-CMP), guanosine-5'-monophosphate (5'-GMP), inosine-5'-monophosphate (Y-IMP) and uridine-5'-monophosphate (5'-UMP).…”

Section: Nucleotidesmentioning

confidence: 99%

“…Johnsen et aL (1990) Kiyohara et al (1981) Hidaka and Ishida (1985) Ishida and Hidaka (1987) Goh and Tamura (1980) Goh and Tamura (1980) Ishida and Hidaka (1987) Ishida and Hidaka (1987) Ishida and Hidaka (1987) Acknowledgements I thank Dr Charles Hollingworth for providing the opportunity to write this article. I also thank Scott Brown, Robert Evans, and Chunbo Zhang for technical and Carol Catt for secretarial assistance.…”

mentioning

confidence: 99%

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