“…At the final of the thermal decomposition process, the residue of around 50% refers to the presence of pure AuNPs. These results indicate the protective effect of the extract compounds on the surface of AuNPs 66 . Figure 7 TGA analysis of BRP crude extract and its fractions as well as the biosynthesized AuNPs.…”
Gold nanoparticles (AuNPs) are highlighted due to their low toxicity, compatibility with the human body, high surface area to volume ratio, and surfaces that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis (BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl acetate) and evaluated their structural properties and their potential against microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR) band at 535 nm. The sizes and morphologies were influenced by the BRP sample used in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from BRP extract or its fractions in the synthesis and stabilization of AuNPs. AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all strains tested, showing their efficacy as antimicrobial agents to treat infectious diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3 cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms associated with apoptosis. The results highlight a potential low-cost green method using Brazilian red propolis to synthesize AuNPs, which demonstrated significant biological properties.
“…At the final of the thermal decomposition process, the residue of around 50% refers to the presence of pure AuNPs. These results indicate the protective effect of the extract compounds on the surface of AuNPs 66 . Figure 7 TGA analysis of BRP crude extract and its fractions as well as the biosynthesized AuNPs.…”
Gold nanoparticles (AuNPs) are highlighted due to their low toxicity, compatibility with the human body, high surface area to volume ratio, and surfaces that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis (BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl acetate) and evaluated their structural properties and their potential against microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR) band at 535 nm. The sizes and morphologies were influenced by the BRP sample used in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from BRP extract or its fractions in the synthesis and stabilization of AuNPs. AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all strains tested, showing their efficacy as antimicrobial agents to treat infectious diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3 cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms associated with apoptosis. The results highlight a potential low-cost green method using Brazilian red propolis to synthesize AuNPs, which demonstrated significant biological properties.
“…Inside the bacterial cells, they interact with the DNA and inhibit transcription of DNA strains, which makes growth inhibition and cellular damage [38][39][40]. In the treatment of E. coli and S. aureus with biosynthesized AuNPs using various plant extracts such as Prunus armeniaca, Curcuma pseudomontana, Carica papaya, Solanum nigrum, Areca catechu, similar results were obtained [8,[41][42][43][44].…”
In the present work, we studied the reduction of gold ions into gold nanoparticles using Nigella arvensis leaf extract in the one-step green synthesis method. The formation of N. arvensis gold nanoparticles (NA-GNPs) was confirmed by UV-Vis spectroscopy, XRD, FT-IR and TEM analyses. The XRD pattern confirmed the crystal structure of NA-GNPs, and TEM image showed the small size (3-37 nm) and almost spherical shape of NA-GNPs. NA-GNPs have not shown enhanced antioxidant properties compared to the plant extract while they were active against the clinical isolated bacterial strains. These nanoparticles showed the cytotoxicity effects against H1299 and MCF-7 cancer cell lines with an IC value of 10 and 25 μg/ml, respectively. The extract of N. arvensis contained 212 μg/ml flavonoids and 145 μg/ml phenolic compounds. The contents of total phenolics and flavonoids of biosynthesized NA-GNPs were 68 and 189 μg/ml, respectively. Plant extract and NA-GNPs exhibited a maximum DPPH scavenging activity of 32% and 12%, respectively. The catalytic activity of NA-GNPs against methylene blue was 44%. In conclusion, these results suggest that NA-GNPs can act as a promising candidate for different medical applications produced by cost-effective, eco-friendly and straightforward green method.
“…Group 2 was administered the standard diclofenac at a dose of 50 mg/kg (i.p.). 13 Groups 3-6 were injected with the tested doses of eriocitrin (5,10,15, and 30 mg/kg), respectively. After an interval of 20 minutes, all the animals were intraperitoneally injected with a 1% acetic acid solution and the number of abdominal constrictions were counted after 10 minutes of acetic acid injection and continued for an additional 20 minutes.…”
“…8,9 Natural products have been shown to play an important role in the discovery of analgesic drugs. 10,14 Different chemical moieties having potent antinociceptive effects have been obtained from natural sources, resulting in novel lead compound classes for designing of analgesic drugs. 15,16 Among natural compounds, flavonoids are considered important phytochemical classes that displayed distinct pharmacological properties.…”
Background: Postoperative pain remains a major clinical problem as there are limited analgesic strategies that have been proven to be effective in preventing and relieving this type of pain. Natural products, including flavonoids, have distinct pharmacological properties and play an important role in the discovery of analgesic drugs. Materials and Methods: In this study, the flavonoid eriocitrin (eriodictyol 7-O-rutinoside), which is the main flavonoid in lemon fruit (Citrus limon), was mechanistically investigated for its prospective antinociceptive effect in a mouse model of postoperative pain. The antinociceptive property was evaluated by utilizing both tonic (acetic acid-induced writhing behavior) and phasic (hot-plate) nociception modalities. The hindpaw incisional surgery was performed and hyperalgesia was assessed using von Frey filaments. Results: The tested doses of eriocitrin significantly attenuated (P<0.01, P<0.001) the chemically-induced tonic visceral nociception (5, 10, 15, and 30 mg/kg) and acute phasic thermal nociception (10, 15, and 30 mg/kg). A significant dose-dependent reduction in the incisional nociceptive hyperalgesia was exhibited by eriocitrin, with a marked antinociception observed at doses of 15 mg/kg (P<0.05 during 30-60 minutes) and 30 mg/kg (P<0.05, P<0.01 during 30-120 minutes). Conclusion: The antinociceptive effect of eriocitrin (30 mg/kg) was strongly blocked by the antagonists of the opioid receptor, naltrexone, and GABA A receptor, bicuculline, thereby suggesting the involvement of opioidergic and GABAergic mechanisms in the nociception, reducing proclivity of eriocitrin during transmission of incisional nociception. These results concluded that eriocitrin has a potent antinociceptive effect in postoperative pain conditions, probably mediated through opioid and GABA A receptors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
