Gum-gut axis: The potential role of salivary biomarkers in the diagnosis and monitoring progress of inflammatory bowel diseases

Balto, Hanan Abdulgafour; Al‐Hadlaq, Solaiman M.; Alhadlaq, Abdulmalik; El‐Ansary, Afaf

doi:10.1016/j.sdentj.2022.12.006

Cited by 2 publications

(4 citation statements)

References 65 publications

(87 reference statements)

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“…Thus, employing traditional cultivation methods, Veillonella spp., Prevotella spp., and P. salivae were successfully isolated from both IBD patients and healthy controls. Our results align with previous studies ( Said et al., 2014 ; Z. Xu et al., 2018 ; Abdelbary et al., 2022 ; Balto et al., 2023 ), indicating a higher prevalence of Veillonella spp. and Prevotella spp.…”

Section: Discussionsupporting

confidence: 93%

“…Our results align with previous studies (Said et al, 2014;Z. Xu et al, 2018;Abdelbary et al, 2022;Balto et al, 2023), indicating a higher prevalence of Veillonella spp. and Prevotella spp.…”

Section: High Prevalence Of Veillonella Spp and Prevotella Spp In Ibd...supporting

confidence: 93%

“…The body’s mucosal surfaces, including the gut and oral mucosa, are colonized by a complex ecosystem ( Chattopadhyay et al., 2019 ). Given the concept that the gut mucosa can be viewed as a continuum of the oral mucosa, thereby bridging the gap between the mouth and the gastrointestinal tract, it is conceivable that the oral cavity might serve as a reservoir for both intestinal commensals and potential pathogens ( Braga and Squier, 1980 ; Bartlett et al., 2020 ; Balto et al., 2023 ). It was previously suggested that swallowed oral microbes may disrupt the homeostasis of the gut microbiota leading to dysbiosis ( Donoff et al., 2014 ; Elad et al., 2019 ; Bao et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, and significance of salivary Veillonella spp., Prevotella spp., and Prevotella salivae in patients with inflammatory bowel disease

Hammad,

Conrads,

Abdelbary

2023

Front. Cell. Infect. Microbiol.

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The global prevalence of inflammatory bowel disease (IBD) is on the rise, prompting significant attention from researchers worldwide. IBD entails chronic inflammatory disorders of the intestinal tract, characterized by alternating flares and remissions. Through high-throughput sequencing, numerous studies have unveiled a potential microbial signature for IBD patients showing intestinal enrichment of oral-associated bacteria. Simultaneously, the oral microbiome can be perturbed by intestinal inflammation. Our prior investigation, based on 16S rRNA amplicon sequencing, underscored elevated abundance of Veillonella spp. and Prevotella spp. in the salivary microbiomes of IBD patients. Noteworthy, Prevotella salivae emerged as a distinct species significantly associated with IBD. P. salivae is an under-recognized pathogen that was found to play a role in both oral and systemic diseases. In this study, we delve deeper into the salivary microbiomes of both IBD patients and healthy controls. Employing diverse cultivation techniques and real-time quantitative polymerase chain reactions (RT-qPCR), we gauged the prevalence and abundance of Veillonella spp., Prevotella spp., and P. salivae. Our isolation efforts yielded 407 and 168 strains of Veillonella spp., as well as 173 and 90 strains of Prevotella spp., from the saliva samples of IBD patients and healthy controls, respectively. Veillonella-vancomycin agar emerged as the discerning choice for optimal Veillonella spp. cultivation, while Schaedler kanamycin-vancomycin agar proved to be the most suitable medium for cultivating Prevotella spp. strains. Comparing our RT-qPCR findings to the previous 16S rRNA amplicon sequencing data, the results corroborated the higher abundance of Veillonella spp., Prevotella spp., and P. salivae in the saliva of IBD patients compared to healthy controls. However, it’s worth noting that in contrast to RT-qPCR, the 16S rRNA amplicon sequencing data revealed greater absolute abundance of all three bacterial groups in both IBD patients and controls.

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Section: Discussionsupporting

confidence: 93%

Section: High Prevalence Of Veillonella Spp and Prevotella Spp In Ibd...supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, and significance of salivary Veillonella spp., Prevotella spp., and Prevotella salivae in patients with inflammatory bowel disease

Hammad,

Conrads,

Abdelbary

2023

Front. Cell. Infect. Microbiol.

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“…There is increasing evidence that the oral microbiome and periodontal inflammation play a significant role in systemic diseases, including gut disorders characterized by gut microbial dysbiosis [ 6 , 7 ]. The gut microbiome is the most abundant and diverse microbiome.…”

Section: Introductionmentioning

confidence: 99%

Periodontal Inflammation and Dysbiosis Relate to Microbial Changes in the Gut

Kamer,

Pushalkar,

Hamidi

et al. 2024

Microorganisms

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Periodontal disease (PerioD) is a chronic inflammatory disease of dysbiotic etiology. Animal models and few human data showed a relationship between oral bacteria and gut dysbiosis. However, the effect of periodontal inflammation and subgingival dysbiosis on the gut is unknown. We hypothesized that periodontal inflammation and its associated subgingival dysbiosis contribute to gut dysbiosis even in subjects free of known gut disorders. We evaluated and compared elderly subjects with Low and High periodontal inflammation (assessed by Periodontal Inflamed Surface Area (PISA)) for stool and subgingival derived bacteria (assayed by 16S rRNA sequencing). The associations between PISA/subgingival dysbiosis and gut dysbiosis and bacteria known to produce short-chain fatty acid (SCFA) were assessed. LEfSe analysis showed that, in Low PISA, species belonging to Lactobacillus, Roseburia, and Ruminococcus taxa and Lactobacillus zeae were enriched, while species belonging to Coprococcus, Clostridiales, and Atopobium were enriched in High PISA. Regression analyses showed that PISA associated with indicators of dysbiosis in the gut mainly reduced abundance of SCFA producing bacteria (Radj = −0.38, p = 0.03). Subgingival bacterial dysbiosis also associated with reduced levels of gut SCFA producing bacteria (Radj = −0.58, p = 0.002). These results suggest that periodontal inflammation and subgingival microbiota contribute to gut bacterial changes.

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Gum-gut axis: The potential role of salivary biomarkers in the diagnosis and monitoring progress of inflammatory bowel diseases

Cited by 2 publications

References 65 publications

Isolation, identification, and significance of salivary Veillonella spp., Prevotella spp., and Prevotella salivae in patients with inflammatory bowel disease

Isolation, identification, and significance of salivary Veillonella spp., Prevotella spp., and Prevotella salivae in patients with inflammatory bowel disease

Periodontal Inflammation and Dysbiosis Relate to Microbial Changes in the Gut

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