Gulliver, a long terminal repeat retrotransposon from the genome of the oriental blood fluke Schistosoma japonicum

Laha, Thewarach; Loukas, Alex; Verity, Christiana K.; McManus, Donald P.; Brindley, Paul J.

doi:10.1016/s0378-1119(00)00601-6

Cited by 23 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As described above, like the errantiviruses such as gypsy (32) and ZAM (38), Boudicca may also have a third ORF encoding an envelope and, if so, may likewise be capable of horizontal transmission as an extracellular infectious particle. Boudicca was clearly distinct from Gulliver, an LTR retrotransposon reported previously from the Asian schistosome Schistosoma japonicum (36) (Fig. 6).…”

Section: Resultsmentioning

confidence: 51%

“…Both the evolution and size of this genome may be highly influenced by mobile genetic elements. Indeed, more than half of the schistosome genome appears to be composed of or derived from repetitive sequences, to a large extent from retrotransposable elements (34)(35)(36).…”

mentioning

confidence: 99%

“…Previously characterized schistosome mobile genetic elements include SINE-like retrotransposons (60,18), LTR retrotransposons (36), and at least two families of non-LTR retrotransposons (35). Although active replication of these elements has not been definitively proven, mRNA transcripts encoding reverse transcriptase and endonuclease have been detected (34,36), as has reverse transcriptase activity in schistosome extracts (29), suggesting that at least some of these elements are actively mobile within the genome.…”

mentioning

confidence: 99%

“…Although active replication of these elements has not been definitively proven, mRNA transcripts encoding reverse transcriptase and endonuclease have been detected (34,36), as has reverse transcriptase activity in schistosome extracts (29), suggesting that at least some of these elements are actively mobile within the genome. Indeed, actively replicating mobile genetic elements in other platyhelminths have been described as RNA intermediates (4) and DNA transposons (3,53).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Boudicca , a Retrovirus-Like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni

Copeland

Brindley

Heyers

et al. 2003

J Virol

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 51%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Boudicca , a Retrovirus-Like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni

Copeland

Brindley

Heyers

et al. 2003

J Virol

Self Cite

View full text Add to dashboard Cite

“…In schistosomes, more than half of the genome appears to be composed of, or derived from, repetitive sequences, to a large extent from retrotransposable elements (Brindley 2005, Copeland et al 2005b. Schistosome MGEs include SINE-like retroposons (Spotila et al 1989, Drew & Brindley 1995, long terminal repeat (LTR) retrotransposons (Laha et al 2001, Foulk et al 2002, Copeland et al 2003, 2005a, DeMarco et al 2004, non-LTR retrotransposons (Laha et al 2002a, b, Brindley et al 2003, and DNA transposons related to bacterial IS1016 insertion sequences (Feschotte 2004). The long terminal repeat retrotransposable elements, i.e.…”

mentioning

confidence: 99%

Identification of the Boudicca and Sinbad retrotransposons in the genome of the human blood fluke Schistosoma haematobium

Copeland

Lewis

Brindley

2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Schistosomes have a comparatively large genome, estimated for Schistosoma mansoni to be about 270 megabase pairs (haploid genome). Recent findings have shown that mobile genetic elements constitute significant proportions of the genomes of S. mansoni and S. japonicum. Much less information is available on the genome of the third major human schistosome, S. haematobium. In order to investigate the possible evolutionary origins of the S. mansoni long terminal repeat retrotransposons Boudicca and Sinbad, several genomes were searched by Southern blot for the presence of these retrotransposons. These included three species of schistosomes, S. mansoni, S. japonicum, and S. haematobium, and three related platyhelminth genomes, the liver flukes Fasciola hepatica and Fascioloides magna and the planarian, Dugesia dorotocephala. In addition, Homo sapiens and three snail host genomes, Biomphalaria glabrata, Oncomelania hupensis, and Bulinus truncatus, were examined for possible indications of a horizontal origin for these retrotransposons. Southern hybridization analysis indicated that both Boudicca and Sinbad were present in the genome of S. haematobium. Furthermore, low stringency Southern hybridization analyses suggested that a Boudicca-like retrotransposon was present in the genome of B. truncatus, the snail host of S. haematobium.Key words: schistosome -long terminal report retrotransposon -mobile genetic element -reverse transcriptase -BulinusThe life cycle of schistosomes, diecious trematodes of the order Digenea, involves parasitism of both humans and genera of aquatic or amphibious snails specific to each species: Biomphalaria for Schistosoma mansoni, Oncomelania for S. japonicum, and Bulinus for S. haematobium. In the case of S. mansoni and S. japonicum, adult worms inhabit the portal system blood vessels and mesenteric veins of the intestines of their human hosts. Much progress has been made toward sequencing and sequence annotation of the genomes of S. mansoni and S. japonicum (El-Sayed et al. 2004), including characterization of much of their transcriptomes (Hu et al. 2003, Verjovski-Almeida et al. 2003, and the construction of a bacterial artificial chromosome (BAC) library representing an eight-fold coverage of the S. mansoni genome (Le Paslier et al. 2000). In contrast to the burgeoning wealth of sequence information for S. mansoni and S. japonicum, relatively little is known about the genome sequence of S. haematobium; less than seventy S. haematobium nucleotide sequences were present in S. haematobium by physical measurement (Hilyer 1974, Rollinson et al. 1997) and their chromosomal karyotypes are similar in appearance .Mobile genetic elements (MGEs) represent a major force driving the evolution of eukaryotic genomes (Charlesworth et al. 1994, Kidwell & Lisch 1997, Kazazian 2004 and play an important role in the establishment of genome size (Petrov et al. 2000). In schistosomes, more than half of the genome appears to be composed of, or derived from, repetitive sequences, to a large extent from retrotrans...

show abstract

Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis)

Wang

Bao

et al. 2007

Genetica

View full text Add to dashboard Cite

Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons were cloned from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis). The total length of the CFG1 element is 4826 bp, including 5'-LTR (192 bp), the entire ORF (4047 bp) and 3'-LTR (189 bp). The entire ORFs of both CFG1 and PYG1 elements are composed of 1348 aa and do not have any frameshifts. Their closest relative is Jule element from the poeciliid fish (Xiphophorus maculatus). On average, the diploid genome of C. farreri contains approximately 84 copies of CFG1 elements. We summarize the major features of CFG1, PYG1 and other elements of Mag lineage of the Ty3/Gypsy group. mRNA expression of CFG1 element in larvae increases gradually before the gastrulae stage and decreases gradually afterward, whereas in adductor such expression in adductor muscle and digestive gland are lower than those in other tissues. Overall, mRNA expression of CFG1 element in the early larvae is significantly higher than that in adult tissues. In muscle tissue, while the promoter and partial GAG domain of CFG1 element are unmethylated, the partial RT domain is highly methylated. These results suggest that CFG1 expression may be controlled by a post-transcriptional gene silencing mechanism that is associated with coding-region (RT domain) methylation.

show abstract

Gulliver, a long terminal repeat retrotransposon from the genome of the oriental blood fluke Schistosoma japonicum

Cited by 23 publications

References 25 publications

Boudicca , a Retrovirus-Like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni

Boudicca , a Retrovirus-Like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni

Identification of the Boudicca and Sinbad retrotransposons in the genome of the human blood fluke Schistosoma haematobium

Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis)

Contact Info

Product

Resources

About