Guinea pig heterophil and eosinophil peroxidase

Desser, Richard K.; Himmelhoch, S.; Evans, Warren H.; Januska, Margaret; Mage, Michael G.; Shelton, Emma

doi:10.1016/0003-9861(72)90164-6

Cited by 131 publications

(47 citation statements)

References 21 publications

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“…Circumference was measured around the greatest portion of the muscle belly with a flexible tape measure. After measurement of circumference mice were deeply anesthetized with sodium pentobarbital, guillotined, and the muscle removed and processed for neutrophilic activity using myeloperoxidase assay (Desser et al, 1972). We analyzed muscle 24 h (n=8 ASIC3−/−; n=7 ASIC3+/+), 1 week (n=7 ASIC3−/−; n=8 ASIC3+/+), and 2 weeks (n=7 ASIC3−/−; n=7 ASIC3 +/+) after induction of inflammation.…”

Section: Measurement Of Inflammationmentioning

confidence: 99%

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Sluka

Radhakrishnan

Benson

et al. 2007

Pain

166

160

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Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3−/− and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3−/−mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/ + mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3−/− mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3−/− mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult.

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Section: Measurement Of Inflammationmentioning

confidence: 99%

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Sluka

Radhakrishnan

Benson

et al. 2007

Pain

166

160

View full text Add to dashboard Cite

show abstract

“…Miyeloperoksidaz aktivitesi nötrofilik infiltrasyonun bir indeksi olarak akciğer doku homojenatlarında Desser ve arkadaşlarının spektrofotometrik yöntemiyle [21] belirlendi. 300 mg doku, 5 mL 0.02 M EDTA tamponu içerisinde homojenize edildi.…”

Section: Miyeloperoksidaz (Mpo) öLçümüunclassified

Effects of acetylsalicylate overdose on lipopolysaccharide-induced lung injury in rats: the role of the VEGF

Akyüz¹,

Aydın²,

Aydın³

et al. 2012

Turk J Bioch

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“…MPO activity was determined according to the method described by Desser et al 23 Briefly, 10 mL WF or enzyme solution was mixed with 290 mL substrate solution, containing 100 mmol/L Guaiacol (Sigma-Aldrich) and 0.017% (w/w) H 2 O 2 in 50 mmol/L potassium phosphate buffer ( pH 7.0). Enzyme standards containing 0.16 U/mL up to 10.0 U/mL MPO (Sigma-Aldrich) were prepared for calibration.…”

Section: Determination Of Mpo Activitymentioning

confidence: 99%

Analysis of myeloperoxidase activity in wound fluids as a marker of infection

et al. 2013

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Background: Neutrophilic polymorphonuclear leukocytes play a crucial role in the host defence against bacterial and fungal infections. They participate in the inflammatory response through the liberation of peptides and enzymes like myeloperoxidase (MPO). Therefore, MPO has a potential as a marker enzyme for the diagnosis of wound infection. Methods: Substrate specificities and reaction pathways of MPO were investigated for new MPO substrates: crystal violet, leuco crystal violet, fast blue RR (4-benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt) and various systematically substituted model substrates based on 2,7-dihydroxy-1-(4-hydroxyphenylazo)naphtalene-3,6-disulphonic acid. In addition, fast blue RR was covalently bound to siloxanes allowing immobilization of the substrate, while cellobiosedehydrogenase was integrated for generation of hydrogen peroxide required by MPO. Results: Elevated concentrations of MPO were found in infected wounds compared with non-infected wounds (92.2 + 45.0 versus 1.9 + 1.8 U/mL). Various soluble and immobilized substrates were oxidized by MPO in wound samples and the influence of substrate structure and reaction pathways were elucidated for selected compounds. Conclusions: Incubation of different MPO substrates with infected wound fluid samples resulted in a clear colour change in the case of elevated MPO concentrations, thus allowing early diagnosis of wound infection.

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Guinea pig heterophil and eosinophil peroxidase

Cited by 131 publications

References 21 publications

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Effects of acetylsalicylate overdose on lipopolysaccharide-induced lung injury in rats: the role of the VEGF

Analysis of myeloperoxidase activity in wound fluids as a marker of infection

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