Guilt by Association: A Phenotype-Based View of the Plant Phosphoinositide Network

Gerth, Katharina; Lin, Feng; Menzel, Wilhelm; Krishnamoorthy, Praveen; Stenzel, Irene; Heilmann, Mareike; Heilmann, Ingo

doi:10.1146/annurev-arplant-042916-041022

Cited by 67 publications

(86 citation statements)

References 149 publications

(107 reference statements)

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“…These analyses did not indicate a significant difference in PtdIns(4)P abundance between wild type, pi4kb1 and pi4kb2 single mutants, the double mutant, or complemented lines ectopically expressing either PI4Kb1 or PI4Kb2 from their respective intrinsic promoters in the pi4kb1 pi4kb2 double mutant background (Appendix Fig S5). Given that in yeast the knockdown of the PI4K, Pik1p, inhibits the recruitment of clathrin to the Golgi (Gloor et al, 2010) and that plant phosphoinositides have previously been linked to clathrin dynamics (Ischebeck et al, 2013;Gerth et al, 2017), we hypothesized that clathrin dynamics may be affected in the Arabidopsis pi4kb1 pi4kb2 double mutant. Interestingly, the association of the 2xmCherry FAPP1-PH with the trans-Golgi-network (TGN) was also reduced in the pi4kb1 pi4kb2 double mutant ( Fig 4A, right plot).…”

Section: Of 26mentioning

confidence: 99%

A dual role for cell plate‐associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis

et al. 2019

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Plant cytokinesis involves membrane trafficking and cytoskeletal rearrangements. Here, we report that the phosphoinositide kinases PI4Kβ1 and PI4Kβ2 integrate these processes in Arabidopsis thaliana (Arabidopsis) roots. Cytokinetic defects of an Arabidopsis pi4kβ1 pi4kβ2 double mutant are accompanied by defects in membrane trafficking. Specifically, we show that trafficking of the proteins KNOLLE and PIN2 at the cell plate, clathrin recruitment, and endocytosis is impaired in pi4kβ1 pi4kβ2 double mutants, accompanied by unfused vesicles at the nascent cell plate and around cell wall stubs. Interestingly, pi4kβ1 pi4kβ2 plants also display ectopic overstabilization of phragmoplast microtubules, which guide membrane trafficking at the cell plate. The overstabilization of phragmoplasts in the double mutant coincides with mislocalization of the microtubule‐associated protein 65‐3 (MAP65‐3), which cross‐links microtubules and is a downstream target for inhibition by the MAP kinase MPK4. Based on similar cytokinetic defects of the pi4kβ1 pi4kβ2 and mpk4‐2 mutants and genetic and physical interaction of PI4Kβ1 and MPK4, we propose that PI4Kβ and MPK4 influence localization and activity of MAP65‐3, respectively, acting synergistically to control phragmoplast dynamics.

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Section: Of 26mentioning

confidence: 99%

A dual role for cell plate‐associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis

et al. 2019

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“…The reporters enable visualization of the subcellular distribution of various lipids including PIPs, diacylglycerol (DAG) and PA in the cytoplasmic leaflet of plant membranes (Helling et al, 2006;Vermeer et al, 2006Vermeer et al, , 2009Vermeer et al, , 2017Van Leeuwen et al, 2007;Vermeer & Munnik, 2013;Potock y et al, 2014;Simon et al, 2014;. In combination with plant mutants impaired in PIP, DAG and PA homeostasis, the reporters have advanced our understanding of the location and potential roles of these lipids in plant cells and illustrated some fundamental differences in lipid signaling between animal and plant cells Boss & Im, 2012;Gerth et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Accumulation of phosphoinositides in distinct regions of the periarbuscular membrane

Ivanov

Harrison

2018

New Phytologist

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Summary Phosphoinositides and phosphatidic acid are small anionic lipids that comprise a minor proportion of total membrane lipids in eukaryotic cells but influence a broad range of cellular processes including endomembrane trafficking, signaling, exocytosis and endocytosis. To investigate the spatial distribution of phosphoinositides during arbuscular mycorrhizal symbiosis, we generated fluorescent reporters of PI(4,5)P2 and PI4P, as well as phosphatidic acid and diacylglycerol and used them to monitor lipid distribution on the cytoplasmic side of membrane bilayers in colonized cortical cells. The PI4P reporter accumulated strongly on the periarbuscular membrane (PAM) and transiently labeled Golgi bodies, while the PA reporter showed differential labeling of endomembranes and the PAM. Surprisingly, the PI(4,5)P2 reporter accumulated in small, discrete regions of the PAM on the arbuscule trunks, frequently in two regions on opposing sides of the hypha. A mutant reporter with reduced PI(4,5)P2 binding capacity did not show these accumulations. The PI(4,5)P2‐rich regions were detected at all phases of arbuscule development following branching, co‐localized with membrane marker proteins potentially indicating high membrane bilayer content, and were associated with an alteration in morphology of the hypha. A possible analogy to the biotrophic interfacial membrane complex formed in rice infected with Magnaporthe orzyae is discussed.

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“…Polyphosphoinositides (PPIs) play critical roles in intracellular signaling pathways and membrane trafficking (Munnik and Nielsen, 2011;Gerth et al, 2017). In particular, phosphatidylinositol 3-P (PI3P) is important for endosomal, vacuolar/lysosomal, and autophagic trafficking through recruitment of effector proteins (Schink et al, 2013).…”

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Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

et al. 2017

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Phosphatidylinositol 3-P (PI3P) is a signaling molecule that controls a variety of processes in endosomal, autophagic, and vacuolar/lysosomal trafficking in yeasts and mammals. Vacuolar protein sorting 34 (Vps34) is a conserved PI3K present in multiple complexes with specific functions and regulation. In yeast, the PI3K complex II consists of Vps34p, Vps15p, Vps30p/ Atg6p, and Vps38p, and is essential for vacuolar protein sorting. Here, we describe the Arabidopsis (Arabidopsis thaliana) homolog of yeast Vps38p and human UV radiation resistance-associated gene protein. Arabidopsis VPS38 interacts with VPS30/ATG6 both in yeast and in planta. Although the level of PI3P in Arabidopsis vps38 mutants is similar to that in wild type, vps38 cells contain enlarged multivesicular endosomes and fewer organelles enriched in PI3P than the wild type. The vps38 mutants are defective in the trafficking of vacuolar cargo and its receptor VACUOLAR SORTING RECEPTOR2;1. The mutants also exhibit abnormal cytoplasmic distributions of endocytic cargo, such as auxin efflux carriers PINFORMED1 (PIN1) and PIN2. Constitutive autophagy is normal in the mutants but starvation-induced autophagy was slightly inhibited. We conclude that Arabidopsis VPS38 is dispensable for autophagy but essential for efficient targeting of biosynthetic and endocytic cargo to the vacuole.

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Guilt by Association: A Phenotype-Based View of the Plant Phosphoinositide Network

Cited by 67 publications

References 149 publications

A dual role for cell plate‐associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis

A dual role for cell plate‐associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis

Accumulation of phosphoinositides in distinct regions of the periarbuscular membrane

Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

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