“…The following clinical prognostic features [2,6,13,17,[20][21][22] were assessed in relation to long term disability: age of onset, sex, degree and time to maximal impairment, duration of plateau phase, time to onset of recovery, motor, sensory, respiratory and cranial nerve impairment, and treatment. We also assessed the following laboratory data obtained during the acute phase: increased CSF protein levels (> 50 mg/dL); serum anti-ganglioside GM1, GD1a, GM2 and GQ1b IgG antibodies (> 1/320) by enzyme-linked immunoabsorbent assay (ELISA) [4]; serum anti-Campylobacter jejuni antibodies by ELISA and immunoblot [19]; serum antibodies to the following virus measured by ELISA according to manufacturers' instructions: HSV-1/HSV-2 IgG (Herpes 1/2 IgG, Bio-Rad Lab., Milan, Italy) and IgM (HSV IgM, Bio-Rad); CMV IgG (CMV IgG, Bio-Rad) and IgM (CMV IgM, Bio-Rad).…”