Guiding Stem Cell Differentiation into Oligodendrocytes Using Graphene‐Nanofiber Hybrid Scaffolds

Shah, Shreyas; Yin, Perry T.; Uehara, Thiers M.; Chueng, Sy-Tsong Dean; Yang, Letao; Lee, Ki‐Bum

doi:10.1002/adma.201400523

Cited by 275 publications

(247 citation statements)

References 36 publications

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“…Another important factor to consider is the scaffold chemistry which has a profound effect on cellular response (Saha et al 2007;Kharaziha et al 2013;Shah et al 2014;Collins & Birkinshaw 2013). …”

Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…Moreover, the differentiation of mesenchymal stem cells and PC12 cells into osteo-and neuro-like cells was enhanced with the incorporation of 0.3 and 0.5 wt% GO in PCL nanofibers. Shah et al [45] also observed that GO coated PCL nanofibers provided the physical cues for selective differentiation of neural stem cells into mature oligodendrocytes. Finally, Chaudhuri et al [145,146] stated excellent differentiation of myoblasts on GO/PCL scaffold meshes.…”

Section: Pcl Filmsmentioning

confidence: 95%

“…GO stimulated myogenic differentiation 0 and 2 wt% GO/PLGA [29] ESM by electrospinning PC12 2 wt% GO improved Tg and mechanical properties more than 1 wt% GO. 2 wt% GO also improved hydrophilicity, neuronal cell viability, and proliferation 0-1 wt% GO/PCL [144] ESM by electrospinning mMSCs, PC12 0.3 and 0.5 wt% GO dramatically enhanced differentiation of MSC and PC12 cells 0.1-1 mg ml À1 GO/PCL [45] ESM by electrospinning neural stem cells GO/PCL promoted differentiation of neural stem cells into mature oligodendrocytes.…”

Section: Natural Polymersmentioning

confidence: 99%

Graphene Oxide/Polymer‐Based Biomaterials

2017

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Since its discovery in 2004, derivatives of graphene have been developed and heavily investigated in the field of tissue engineering. Among the most extensively studied forms of graphene, graphene oxide (GO), and GO/ polymer-based nanocomposites have attracted great attention in various forms such as films, 3D porous scaffolds, electrospun mats, hydrogels, and nacre-like structures. In this review, the most actively investigated GO/ polymer nanocomposites are presented and discussed, these nanocomposites are based on chitosan, cellulose, starch, alginate, gellan gum, poly(vinyl alcohol) (PVA), poly(acrylamide), poly(e-caprolactone) (PCL), poly(lactic acid) (PLLA), poly(lactide-co-glycolide) (PLGA), gelatin, collagen, and silk fibroin (SF). The biological and mechanical performance of such nanocomposites are comprehensively scrutinized and ongoing research questions are addressed. The analysis of the literature reveals overall the great potential of GO/ polymer nanocomposites in tissue engineering strategies and indicates also a series of challenges requiring further research efforts.

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“…Weaver et al [43] also explored the potential of a GO/PEDOT (poly 3,4-ethylenedioxythiophene) nanocomposite films for NSCs differentiation in vitro and showed that they GO composites promoted neuronal differentiation while only PEDOT surfaces lead more toward oligodendrocyte differentiation. On the contrary, Shah et al [44] used nanofibrous scaffolds with graphene to guide NSC differentiation to oligodendrocytes without any differentiating agent. They observed that coating with a higher concentration of GO promoted differentiation toward mature oligodendrocytes.…”

Section: Discussionmentioning

confidence: 99%

Graphene oxide has a neuroprotective effect against glutamate-induced excitoxicity on B35 neuroblastoma cell line

Kayhan¹,

Taşdemir²,

İlhan³

et al. 2015

Anatomy

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Glutamate is one of the main excitatory neurotransmitters in the central nervous system (CNS), involved in neural transmission, development, differentiation and plasticity. It plays a vital role in neural pathways for cognition, memory and learning, and synapse induction and elimination during development, cell migration, differentiation and death. Glutamate is continuously released from neurons and uptaken from extracellular fluid. AbstractObjectives: Graphene is a quasi-two-dimensional material with unique electrical and chemical properties. In terms of biomedical applications of graphene, nervous system would be an ideal breakthrough model because neural cells are electroactive. Extreme glutamate concentrations cause excitotoxicity. In this study, we aimed to investigate if graphene can increase the resistance to glutamate stress in B35 rat neuroblastoma cells as a cultured cell model for central nervous system neurons.Methods: B35 neuroblastoma cells were grown in DMEM-F12 growth medium containing 10% fetal bovine serum. Graphene oxide (GO) powder was coated onto glass slides with chitosan as a thin film. B35 cells were cultured on GO films. Cells cultivated on glass slides were used as controls. After 24 h of cell culture, L-glutamine induced excitotoxicity was imposed on B35 cells. After 24 h of glutamate-induced stress, cell morphology was examined by scanning electron microscopy. Cell viability was measured with MTT assay. Results:The effects of glutamate stress on cell viability were visible as early as 1 h. The cell viability on GO films was higher than that on glass slides, and cells recovered from stress within 6 h on GO surfaces. After 24 h, viability on glass surfaces was 54% lower than that on GO surfaces; these findings were supported with cell morphology observations. Conclusion:The results of this study showed that GO has a protective role in reducing glutamate-induced excitotoxicity in B35 cell culture, indicating a potential use of GO for treatment of excitotoxicity induced neurodegenerative diseases.

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Guiding Stem Cell Differentiation into Oligodendrocytes Using Graphene‐Nanofiber Hybrid Scaffolds

Cited by 275 publications

References 36 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Graphene Oxide/Polymer‐Based Biomaterials

Graphene oxide has a neuroprotective effect against glutamate-induced excitoxicity on B35 neuroblastoma cell line

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