Guidelines for the Care and Use of Laboratory Animals in Biomedical Research

Jones‐Bolin, Susan

doi:10.1002/0471141755.pha04bs59

Cited by 94 publications

(58 citation statements)

References 24 publications

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“…All animal experiments were approved by the Animal Study committee of Kunming Medical University (approval no. KMMU 2015007) and were conducted according to the requirements of the National Institutes of Health Guidelines for care and use of laboratory animals (24). A total of 62 female 3-month-old Sprague-Dawley rats (270±15 g; Dossy Co.) were maintained in standard conditions with a controlled temperature (21-23˚C) and a strict 12:12 h light: dark cycle.…”

Section: Methodsmentioning

confidence: 99%

Osteoking improves OP rat by enhancing HSP90‑β expression

et al. 2020

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Osteoporosis (OP) is a chronic bone disease that affects individuals worldwide. Osteoporosis is primarily asymptomatic, and patients with OP suffer from pain, inconvenience, economic pressure and osteoporotic fracture (OPF). Osteoking, a Traditional chinese Medicine compound that originates from the Yi ethnic group, has been used for a number of years to treat fractures. In our previous study, osteoking exhibited therapeutic effects on rats with OPF by promoting calcium deposition. Based on bioinformatics and network pharmacology analyses of a component-target-disease database, heat shock protein HSP 90-β (HSP90-β), also known as HSP90-β, was identified to be a key target of osteoking in OP. High HSP90-β expression levels were observed in osteoporotic rats and rat bone mesenchymal stem cells (rBMScs) following osteoking treatment. After 12 weeks of administration in vivo, there was increased bone mineral density (BMd) (P<0.05), increased bone alkaline phosphatase (P<0.05), and improved bone microstructure in the osteoking group compared with those of the negative control group. In vitro, increased calcium deposition in rBMScs was observed after 4 weeks of osteoking treatment. These results suggest that the mechanisms of osteoking are closely associated with HSP90-β and activate the bone morphogenetic protein (BMP) signalling pathway, primarily through BMP-2. Osteoking treatment improves OP in rats by enhancing HSP90-β expression.

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Section: Methodsmentioning

confidence: 99%

Osteoking improves OP rat by enhancing HSP90‑β expression

et al. 2020

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“…The animals’ health status was monitored throughout the experiments by a health surveillance programme according to Institutional Animal Care and Use Committee (IACUC) guidelines [ 21 ]. The rabbits were free of all viral, bacterial, and parasitic pathogens listed in the IACUC recommendations.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Therapeutic Efficacy of Sequential Therapy Involving Percutaneous Microwave Ablation in Combination with 131I-Hypericin Using the VX2 Rabbit Breast Solid Tumor Model

et al. 2015

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PurposeCombination of percutaneous microwave ablation (PMWA) and intravenous injection of 131I-hypericin(IIIH) may bear potential as a mini-invasive treatment for tumor. The objective of this study was to assess the effect of PMWA and IIIH in breast tumor growth.MethodsTen New Zealand White rabbits bearing VX2 breast carcinomas were randomly divided into two groups (each 5 examples) and processed using PMWA followed by IIIH and IIIH alone. The IIIH activity was evaluated using planar scintigraphy, autoradiography and biodistribution analysis. The maximum effective safe dose of IIIH was found through 48 rabbits with VX2 breast tumor, which were randomized into six groups (n=8 per group). Subsequently, a further 75 rabbits bearing VX2 breast solid tumors were randomly divided into five groups (each 15 examples) and treated as follows: A, no treatment group; B, PMWA alone; C, IIIH alone; D, PMWA+IIIH×1 (at 8 h post-PMWA); and E, PMWA+IIIH×2 (at 8 h and at 8 days post-PMWA). The therapeutic effect was assessed by measurement of tumor size and performation of positron emission tomography/computed tomograph (PET/CT) scans, liver and renal function tests and Kaplan-Meier survival analysis.ResultsThe planar scintigraphy findings suggested a significant uptake of 131I in necrotic tumor tissue. The autoradiography gray scales indicated higher selective uptake of IIIH by necrotic tissue, with significant differences between the groups with and those without necrotic tumor tissue (P<0.05). The maximum effective safe dose of IIIH was 1mCi/kg. The PET/CT scans and tumor size measurement suggested improvements in treatment groups at all time points (P<0.01). Significant differences were detected among Groups A, B, D and E (P<0.05). Lower levels of lung metastasis were detected in Groups D and E (P<0.05). There were no abnormalities in liver and renal functions tests or other reported side effects.ConclusionIIIH exhibited selective uptake by necrotic tumor tissue. Sequential therapy involving PMWA+IIIH was successfully inhibiting tumor growth and prolonging survival.

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“…The mice received gentamycin in the drinking water (800,000 U/l) until 1 week following the implantation. All in vivo experiments were conducted according to the Guide for the Care and Use of Laboratory Animals (16) and approved by the Animal Care and Use Committee of Nanjing Medical University.…”

Section: Methodsmentioning

confidence: 99%

Screening and analysis of breast cancer genes regulated by the human mammary microenvironment in a humanized mouse model

et al. 2016

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Abstract. Tumor microenvironments play critical regulatory roles in tumor growth. Although mouse cancer models have contributed to the understanding of human tumor biology, the effectiveness of mouse cancer models is limited by the inability of the models to accurately present humanized tumor microenvironments. Previously, a humanized breast cancer model in severe combined immunodeficiency mice was established, in which human breast cancer tissue was implanted subcutaneously, followed by injection of human breast cancer cells. It was demonstrated that breast cancer cells showed improved growth in the human mammary microenvironment compared with a conventional subcutaneous mouse model. In the present study, the novel mouse model and microarray technology was used to analyze changes in the expression of genes in breast cancer cells that are regulated by the human mammary microenvironment. Humanized breast and conventional subcutaneous mouse models were established, and orthotopic tumor cells were obtained from orthotopic tumor masses by primary culture. An expression microarray using Illumina HumanHT-12 v4 Expression BeadChip and database analyses were performed to investigate changes in gene expression between tumors from each microenvironment. A total of 94 genes were differentially expressed between the primary cells cultured from the humanized and conventional mouse models. Significant upregulation of genes that promote cell proliferation and metastasis or inhibit apoptosis, such as SH3-domain binding protein 5 (BTK-associated), sodium/chloride cotransporter 3 and periostin, osteoblast specific factor, and genes that promote angiogenesis, such as KIAA1618, was also noted. Other genes that restrain cell proliferation and accelerate cell apoptosis, including tripartite motif containing TRIM36 and NES1, were downregulated. The present results revealed differences in various aspects of tumor growth and metabolism between the two model groups and indicated the functional changes specific to the human mammary microenvironment. IntroductionOver previous years, tumor microenvironments have been recognized to play important roles in regulating tumor progression (1). The tumor microenvironment includes interwoven stroma and cells, such as fibroblasts, adipocytes, myoepithelial cells, inflammatory cells and endothelial cells, in addition to the secreted factors of the stroma and cells. The tumor microenvironment promotes tumor growth, stimulates angiogenesis, increases inflammatory response and induces metastasis (1-4).Mouse models of human cancer play a crucial role in cancer research and the screening of anticancer agents (5,6). However, although certain agents show consistent and potent anticancer activity in mouse models, the response rate among patients in phase I clinical trials is <10% (7,8). Therefore, it has been hypothesized that current mouse models cannot accurately mimic the humanized tumor microenvironment (9).Previously, a humanized mouse model of breast cancer, in which normal human breast tissue was impl...

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Guidelines for the Care and Use of Laboratory Animals in Biomedical Research

Cited by 94 publications

References 24 publications

Osteoking improves OP rat by enhancing HSP90‑β expression

Osteoking improves OP rat by enhancing HSP90‑β expression

Evaluation of the Therapeutic Efficacy of Sequential Therapy Involving Percutaneous Microwave Ablation in Combination with 131I-Hypericin Using the VX2 Rabbit Breast Solid Tumor Model

Screening and analysis of breast cancer genes regulated by the human mammary microenvironment in a humanized mouse model

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