Guidelines for repeated measures statistical analysis approaches with basic science research considerations

Muhammad, Lutfiyya N.

doi:10.1172/jci171058

Cited by 15 publications

(5 citation statements)

References 24 publications

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“…The conversations with ChatGPT are given in the Supplementary Materials. The tool allowed for the facile application of statistical tests and analyses like the Kruskal-Wallis test [53], Mann-Whitney U test [54] with Bonferroni correction [55], ANOVA (analysis of variance) [56], ANCOVA (analysis of covariance) [57], and multivariate regression analysis.…”

Section: Discussionmentioning

confidence: 99%

The Impact of ZnO and Fe2O3 Nanoparticles on Sunflower Seed Germination, Phenolic Content and Antiglycation Potential

Al-Sudani,

Al-Shammari,

Abed

et al. 2024

Plants

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The enhancement of seed germination by using nanoparticles (NPs) holds the potential to elicit the synthesis of more desired compounds with important biomedical applications, such as preventing protein glycation, which occurs in diabetes. Here, we used 7 nm and 100 nm ZnO and 4.5 nm and 16.7 nm Fe2O3 NPs to treat sunflower seeds. We evaluated the effects on germination, total phenolic content, and the anti-glycation potential of extracted polyphenols. Sunflower seeds were allowed to germinate in vitro after soaking in NP solutions of different concentrations. Polyphenols were extracted, dosed, and used in serum albumin glycation experiments. The germination speed of seeds was significantly increased by the 100 nm ZnO NPs and significantly decreased by the 4.5 nm Fe2O3 NPs. The total phenolic content (TPC) of seeds was influenced by the type of NP, as ZnO NPs enhanced TPC, and the size of the NPs, as smaller NPs led to improved parameters. The polyphenols extracted from seeds inhibited protein glycation, especially those extracted from seeds treated with 7 nm ZnO. The usage of NPs impacted the germination speed and total polyphenol content of sunflower seeds, highlighting the importance of NP type and size in the germination process.

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Section: Discussionmentioning

confidence: 99%

The Impact of ZnO and Fe2O3 Nanoparticles on Sunflower Seed Germination, Phenolic Content and Antiglycation Potential

Al-Sudani,

Al-Shammari,

Abed

et al. 2024

Plants

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show abstract

“…For analyses that included a different number of males and females, homogeneity of variance was assessed using an F test. Lastly, the repeated-measures ANOVA used for our main comparisons of effects of stress or CORT vs control conditions assume sphericity (constant variance across time points/test conditions ( Muhammad, 2023 )). To control for any potential violations of this assumption, p values were corrected using the Huynh-Feldt method.…”

Section: Methodsmentioning

confidence: 99%

Acute stress differentially alters reward-related decision making and inhibitory control under threat of punishment

Laino Chiavegatti,

Floresco

2024

Neurobiology of Stress

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“…Due to the use of continuous independent variables, a linear mixed‐effects model was used to evaluate the effect of canopy openness and koa distance, instead of a repeated measures analysis of variance (ANOVA). The assumption of sphericity was not met for the data, but we concluded it was acceptable to proceed with the analysis because sphericity is not considered to be strictly necessary for mixed models, but compound symmetry is the more important aspect (Armstrong 2017; Muhammad 2023). Linear mixed‐effects models allow for specification of the covariance structure to meet the assumption of compound symmetry.…”

Section: Methodsmentioning

confidence: 99%

Tradeoffs in access to light and root networks of established host trees for restoration planting of the root hemiparasite Santalum paniculatum

Thyroff,

Friday,

Idol

et al. 2024

Restoration Ecology

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Restoration of root hemiparasite trees, such as Hawaiʻi's endemic Santalum species (ʻiliahi), may benefit from underplanting in stands of suitable hosts like the nitrogen‐fixing native tree, Acacia koa (koa). At a pasture site on Hawaiʻi Island previously reforested with koa, we underplanted seedlings of the island‐endemic sandalwood species, Santalum paniculatum, to examine the tradeoff between access to an established root network (distance to the nearest koa tree) under variable overstory shading (8.8–90.1% canopy openness range) during regeneration establishment. We hypothesized that there is an optimal parasite–host spacing and canopy openness that balance parasitic resource transfer with light availability. ʻIliahi seedling survival was 96% with no survival treatment differences. ‘Iliahi seedling growth was positively related to canopy openness but negatively related to the distance to the nearest koa tree, and the slope of these relationships increased over time. Leaf photosynthetic light compensation points, light saturation points, and stomatal density mostly followed similar trends as growth. These results demonstrate that ‘iliahi can be successfully underplanted in an established koa stand, which benefits ‘iliahi plantings and contributes to diversifying initial restoration and reforestation plantings. There appears to be a significant tradeoff in planting distance between benefits from and competition with the host; however, the improvement in growth with increased canopy openness appeared to be much greater than the effect of planting distance. Underplanting into an established host stand with sufficient canopy openness can help restore functionally compatible and abundant ‘iliahi regeneration into forests.

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Guidelines for repeated measures statistical analysis approaches with basic science research considerations

Cited by 15 publications

References 24 publications

The Impact of ZnO and Fe2O3 Nanoparticles on Sunflower Seed Germination, Phenolic Content and Antiglycation Potential

The Impact of ZnO and Fe2O3 Nanoparticles on Sunflower Seed Germination, Phenolic Content and Antiglycation Potential

Acute stress differentially alters reward-related decision making and inhibitory control under threat of punishment

Tradeoffs in access to light and root networks of established host trees for restoration planting of the root hemiparasite Santalum paniculatum

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