Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery

Covington, Brett C.; Seyedsayamdost, Mohammad R.

doi:10.1016/bs.mie.2021.11.020

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Molecular Biology1

Biosynthetic Gene Cluster Activation1

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2023

2024

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Cited by 2 publications

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Section: Molecular Biologymentioning

confidence: 99%

“…Using improved RGMS, several cryptic metabolites from mutant libraries of various Burkholderia species were identified. The authors used transposon mutagenesis instead of UV [156] and MS-based metabolomics instead of a reporter construct [157,158]. Ribosomal engineering as an approach was formulated and actively developed by a group of Japanese researchers under the leadership of Prof. Ochi [151,152].…”

Section: Biosynthetic Gene Cluster Activationmentioning

confidence: 99%

See 1 more Smart Citation

Modern Trends in Natural Antibiotic Discovery

Baranova

Alferova

Korshun

et al. 2023

Life

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Natural scaffolds remain an important basis for drug development. Therefore, approaches to natural bioactive compound discovery attract significant attention. In this account, we summarize modern and emerging trends in the screening and identification of natural antibiotics. The methods are divided into three large groups: approaches based on microbiology, chemistry, and molecular biology. The scientific potential of the methods is illustrated with the most prominent and recent results.

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Section: Molecular Biologymentioning

confidence: 99%