Guideline for flow cytometric immunophenotyping: A report from the national institute of allergy and infectious diseases, division of AIDS

Calvelli, Theresa A.; Denny, Thomas N.; Paxton, Helene; Gelman, Rebecca; Kagan, Jonathan

doi:10.1002/cyto.990140703

Cited by 192 publications

(130 citation statements)

References 27 publications

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“…The diagnosis of asthma and reactive airways disease reported by parents and recorded by WITS medical personnel in the first 1-2 years life of the study subjects suggested an overuse of the diagnosis of asthma as applied to wheezing infants with respiratory syncytial virus or rhinovirus present with asthma-like symptoms (data not shown). 17,[34][35][36] Many of these young infants infected with respiratory viruses, particularly those with underlying allergies, indeed go on to have persistent asthma, but a majority have their symptoms remit in a few years. The WITS program did not provide a long-term evaluation of the persistence of asthma symptoms with measurement of pulmonary function.…”

Section: Discussionmentioning

confidence: 99%

“…16 For flow cytometry, samples were prepared for staining and analysis by means of whole-blood lysis, as previously published. 17 The diagnosis of HIV infection required two positive assays, HIV culture or HIV DNA PCR. Two-to-three negative HIV assays after 1 month of age were required for the assignment of a child to the HIV− group.…”

Section: Laboratory Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Increased incidence of asthma in HIV-infected children treated with highly active antiretroviral therapy in the National Institutes of Health Women and Infants Transmission Study

Foster

McIntosh

Thompson

et al. 2008

Journal of Allergy and Clinical Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Laboratory Studiesmentioning

confidence: 99%

Increased incidence of asthma in HIV-infected children treated with highly active antiretroviral therapy in the National Institutes of Health Women and Infants Transmission Study

Foster

McIntosh

Thompson

et al. 2008

Journal of Allergy and Clinical Immunology

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“…For dual-color flow cytometric analysis, samples were prepared by whole-blood lysis [31]. The same reagent lots were used at all sites (Becton-Dickinson) and consisted of an isotype control (IgG1 and IgG2a), CD45/CD14 (gating reagent), CD4/ CD8 (compensation reagent), CD3/CD4 (CD4 + T lymphocytes), CD3/CD8 (CD8 + T lymphocytes), CD8/CD38 (activated CD8 + T lymphocytes), CD8/HLA-DR (activated CD8 + T lymphocytes), CD3 Ϫ /CD16 + CD56 + (natural killer cells), and CD3 Ϫ / CD19 (B lymphocytes), conjugated to fluorescein isothiocyanate and phycoerythrin, respectively [32].…”

Section: Methodsmentioning

confidence: 99%

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Mekmullica¹,

Brouwers²,

Charurat³

et al. 2009

CLIN INFECT DIS

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“…CD4+ cell counts were performed using standard flow cytometric methods in laboratories certified by the DAIDS Quality Assurance program [7]. Progesterone levels were measured at each visit using an enzyme-modified immunoassay .…”

Section: Methodsmentioning

confidence: 99%

Safety and tolerability of depot medroxyprogesterone acetate among HIV-infected women on antiretroviral therapy: ACTG A5093

et al. 2008

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Background-Concomitant use of antiretrovirals (ARV) and hormonal contraceptives may change the metabolism of each and the resulting safety profiles. We evaluated the safety and tolerability of depot medroxyprogesterone acetate (DMPA) among women on ARV.Study Design-HIV-infected women on selected ARV regimens or no ARV were administered DMPA 150 mg intramuscularly and evaluated for 12 weeks for adverse events, changes in CD4+ count and HIV RNA levels, and ovulation.Results-Seventy evaluable subjects were included, 16 on nucleoside only or no ARV, 21 on nelfinavir-containing regimens, 17 on efavirenz-containing regimens, and 16 on nevirapinecontaining regimens. Nine grade 3 or 4 adverse events occurred in 7 subjects; none were judged related to DMPA. The most common findings possibly related to DMPA were abnormal vaginal bleeding (9, 12.7%), headache (3, 4.2%), abdominal pain, mood changes, insomnia, anorexia, and fatigue, each occurring in 2 (2.9%) subjects. No significant changes in CD4+ count or HIV RNA levels occurred with DMPA. No evidence of ovulation was detected, and no pregnancies occurred. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Conclusions-The clinical profile associated with DMPA administration in HIV-infected women, most on ARV, appears similar to that seen in HIV-uninfected women. DMPA prevented ovulation and did not affect CD4+ counts or HIV RNA levels. In concert with previously published DMPA/ ARV interaction data, these data suggest that DMPA can be used safely by HIV-infected women on the ARV studied. Conflicts of interest

show abstract

Guideline for flow cytometric immunophenotyping: A report from the national institute of allergy and infectious diseases, division of AIDS

Cited by 192 publications

References 27 publications

Increased incidence of asthma in HIV-infected children treated with highly active antiretroviral therapy in the National Institutes of Health Women and Infants Transmission Study

Increased incidence of asthma in HIV-infected children treated with highly active antiretroviral therapy in the National Institutes of Health Women and Infants Transmission Study

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Safety and tolerability of depot medroxyprogesterone acetate among HIV-infected women on antiretroviral therapy: ACTG A5093

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