Guide to Selecting a Biorecognition Element for Biosensors

Morales, M. Ángeles F.; Halpern, Jeffrey Mark

doi:10.1021/acs.bioconjchem.8b00592

Cited by 324 publications

(243 citation statements)

References 130 publications

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“…Furthermore, the paper provides an in-depth survey on methods and applications [94]. Recently, a review on biological and synthetic materials as recognition elements for food safety analysis has been published [95,96] can help to select a suitable recognition element. Because of the wide field of detection principles and applications the review aims to focus on optical biosensing and within these many methods is restricted to biosensors based on direct optical detection.…”

Section: Biomolecular Interaction Analysismentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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Section: Biomolecular Interaction Analysismentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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“…Evaluation of the reproducibility of a surface modification is important to ensure a consistent and reliable response in subsequent characterization steps. 37 Reproducibility of the I40 surface modification was evaluated by comparing the impedance response across multiple unique modification events (N = 6) ( Fig. 5).…”

Section: Elastin-like Polymer Surface Immobilizationmentioning

confidence: 99%

Electrochemical characterization of the stimuli-response of surface-immobilized elastin-like polymers

et al. 2019

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“…There are two main types of impedimetric biosensors-with or without a specific biorecognition element [30]. The most common biorecognition elements used are specific antibodies [39,40]. The key to the successful performance of EIS-based biosensors is how to decrease the non-specific bindings and increase the selectivity.…”

Section: Challenges Of Eis-based Biosensorsmentioning

confidence: 99%

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

Kivirand¹,

Min²,

Rinken³

2019

Biosensors for Environmental Monitoring

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Monitoring of the environment is a global priority due to the close connection between the environmental pollution and human health. Many analytical techniques using various methods have been developed to detect and monitor the levels of pollutants (pesticides, toxins, bacteria, drug residues, etc.) in natural water bodies. The latest trend in modern analysis is to measure pollutants in real-time in the field. For this purpose, biosensors have been employed as cost-effective and fast analytical techniques. Among biosensors, impedance biosensors have significant potential for use as simple and portable devices. These sensors involve application of a small amplitude AC voltage to the sensor electrode and measurement of the in-/out-ofphase current response as a function of frequency integrated with some biorecognition element on the sensing electrodes that can bind to the target, modifying the sensor electrical parameters. However, there are some drawbacks concerning their selectivity, stability, and reproducibility. The aim of this paper is to give a critical overview of literature published during the last decade based on the development issues of impedimetric biosensors and their applicability in water analysis.

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Guide to Selecting a Biorecognition Element for Biosensors

Cited by 324 publications

References 130 publications

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Electrochemical characterization of the stimuli-response of surface-immobilized elastin-like polymers

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

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