Guidance of neural regeneration on the biomimetic nanostructured matrix

Lu, Yen-Pei; Yang, Chih-Hui; Yeh, J. Andrew; Ho, Fu Han; Ou, Yu-Cheng; Chen, Chieh Hsiao; Lin, Ming-Yen; Huang, Keng‐Shiang

doi:10.1016/j.ijpharm.2013.08.006

Cited by 16 publications

(17 citation statements)

References 35 publications

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“…The most basic requirement for a synthetic biomaterial scaffold for cell growth and tissue regeneration (beyond toxicity) is its ability to support cell attachment (Cooke et al, 2010). For this purpose, synthetic biomaterials for neuronal regeneration are typically coated or functionalized with either (a) polymers which are able to interact with the negatively charged cell membrane, (b) reactive layers that can (covalently) bind to the cell surface, or (c) specific cell adhesive molecules able to interact with adhesive receptors at the cell membrane (i.e., integrins) Lu et al, 2014;Akizawa et al, 2016;Hamsici et al, 2017). Positively charged polymers like polylysine (PL), poly(ornithine) (PO), poly(ethyleneimine) (PEI), poly(propyleneimine) (PPI) or poly(allylamine hydrochloride) are traditionally used in neuronal cell cultures (Roach et al, 2010).…”

Section: Biomaterials That Support Neuronal Cell Adhesionmentioning

confidence: 99%

Microenvironments Designed to Support Growth and Function of Neuronal Cells

2018

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Strategies for neural tissue repair heavily depend on our ability to temporally reconstruct the natural cellular microenvironment of neural cells. Biomaterials play a fundamental role in this context, as they provide the mechanical support for cells to attach and migrate to the injury site, as well as fundamental signals for differentiation. This review describes how different cellular processes (attachment, proliferation, and (directional) migration and differentiation) have been supported by different material parameters, in vitro and in vivo. Although incipient guidelines for biomaterial design become visible, literature in the field remains rather phenomenological. As in other fields of tissue regeneration, progress will depend on more systematic studies on cell-materials response, better understanding on how cells behave and understand signals in their natural milieu from neurobiology studies, and the translation of this knowledge into engineered microenvironments for clinical use.

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Section: Biomaterials That Support Neuronal Cell Adhesionmentioning

confidence: 99%

Microenvironments Designed to Support Growth and Function of Neuronal Cells

2018

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“…Posttraumatic or disease caused injury in nervous system is always very debilitating lesion. For this reason, search for new treatment solutions, implants that will match in the best way with neuroregeneration process requirements, is intensively continued [1][2][3][4][5][6][7][8]. The common methodology in tissue engineering is to mimic the architecture of the native extracellular matrix (ECM), which plays a significant role in regulating cellular behaviour [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…For this reason, search for new treatment solutions, implants that will match in the best way with neuroregeneration process requirements, is intensively continued [1][2][3][4][5][6][7][8]. The common methodology in tissue engineering is to mimic the architecture of the native extracellular matrix (ECM), which plays a significant role in regulating cellular behaviour [1][2][3][4][5][6][7]. The interest in fibrous scaffolds for regenerative medicine is based on the electrospun micro-/nanofibers of high resemblance to the hierarchical fibrillary arrangement of the ECM [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Conventionally, electrospun fibrous scaffolds have been fabricated from sole polymer sources [5,11,[19][20][21][22][23][24][25][26][27][28][29][30], but recently combinations of polymers have been used to create polyblend fibers [1,10,. Polyurethanes (PU) and polylactide (PLDL) are known to be used as biodegradable scaffolds (in vitro) [25,30] and implants (in vivo) [37,38].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Olfactory Ensheathing Glial Cells Cultured on Polyurethane/Polylactide Electrospun Nonwovens

Grzesiak

Fryczkowski

Lis

et al. 2015

International Journal of Polymer Science

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The aim of this research was to evaluate novel biomaterials for neural regeneration. The investigated materials were composed of polyurethane (PU) and polylactide (PLDL) blended at three different w/w ratios, that is, 5/5, 6/4, and 8/2 of PU/PLDL. Ultrathin fibrous scaffolds were prepared using electrospinning. The scaffolds were investigated for their applicability for nerve regeneration by culturing rat olfactory ensheathing glial cells. Cells were cultured on the materials for seven days, during which cellular morphology, phenotype, and metabolic activity were analysed. SEM analysis of the fabricated fibrous scaffolds showed fibers of a diameter mainly lower than 600 m with unimportant volume of protrusions situated along the fibers, with nonsignificant differences between all analysed materials. Cells cultured on the materials showed differences in their morphology and metabolic activity, depending on the blend composition. The most proper morphology, with numerous p75 + and GFAP + cells present, was observed in the sample 6/4, whereas the highest metabolic activity was measured in the sample 5/5. However, none of the investigated samples showed cytotoxicity or negatively influenced cellular morphology. Therefore, the novel electrospun fibrous materials may be considered for regenerative medicine applications, and especially when contacting with highly sensitive nervous cells.

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“…Studies have shown that changes in neurite outgrowth lead to changes in the ion channel of the nervous system. 4 Imitating the growth, 5,6 differentiation, and migration of neurons in vitro, especially by constructing a graphical neural network, simulating the model of neural network lesion, and studying the neurites of the graphical neural network, may be the most effective way to solve the basic problems of the pathology of encephalopathy and the mechanism of drug action on the neural network. [7][8][9] At present, the main method of constructing a graphical neural network in vitro is microcontact printing (micro-CP) technology.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic array chip based on excimer laser processing technology for the construction of in vitro graphical neuronal network

Shen

Tian

et al. 2020

Journal of Bioactive and Compatible Polymers

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To construct a graphical neural network in vitro and explore the morphological effects of neural network structural changes on neurons, this study aimed to introduce a method for fabricating microfluidic array chips with different graphical structures based on 248-nm excimer laser one-step etching. Through the comparative analysis of the graphical neural network cultured on our microfluidic array chip with the one on the glass slide, the morphological effects of the neural network on the morphology of the neurons were studied. First, the design of the chip was completed according to the specific structure of the neurons and the simulation of the flow field. The chips were fabricated by excimer laser processing combined with the casting technology. Neurons were cultured on the chip, and a graphical neural network was formed. The growth status of the neural network was analyzed by microscopy and immunofluorescence technology, and compared with the random neural network cultured on glass slides. The results showed that the neurons on the array chips grew in microchannels, and neurites grew along the direction of the channel, interlacing to form a neural network. Furthermore, when the structure of the neural network was graphically changed, the internal neuron morphology changed: on the same culture days, the maximum length of the neurites of the graphical neural network was higher than the average length of the neurites of the random neural network. This research can provide the foundation for the exploration of the neural network mechanism of neurological diseases.

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Guidance of neural regeneration on the biomimetic nanostructured matrix

Cited by 16 publications

References 35 publications

Microenvironments Designed to Support Growth and Function of Neuronal Cells

Microenvironments Designed to Support Growth and Function of Neuronal Cells

Characterization of Olfactory Ensheathing Glial Cells Cultured on Polyurethane/Polylactide Electrospun Nonwovens

Microfluidic array chip based on excimer laser processing technology for the construction of in vitro graphical neuronal network

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