Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend

Schnell, Eva; Klinkhammer, Kristina; Balzer, Simone; Brook, Gary; Klee, Doris; Dalton, Paul D.; Mey, Jörg

doi:10.1016/j.biomaterials.2007.03.009

Cited by 668 publications

(543 citation statements)

References 42 publications

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“…Obviously, this adhesive needs to be non-toxic. To fasten fibers, investigators have used adhesive bandage [1], biocompatible "super glues" [32], silicone glues [33] and silylated glass surfaces [3], as well as other fasteners. In this study, we found that PLGA proved to be a more biocompatible adhesive than bandage and silicone glue, which proved to be toxic to primary neurons.…”

Section: Discussionmentioning

confidence: 99%

“…PLGA is also very easy to use as a fastener for nanofibers. Silylated glass is also compatible with serum-free culture of neurons [34,35] and can be used to fasten fibers [3], but mixing and applying PLGA to glass cover slips is faster and less complicated than silylation.…”

Section: Discussionmentioning

confidence: 99%

“…Aligned electrospun nanofibers powerfully direct the growth of regenerating neurites in vitro, offering promise as scaffolds for peripheral nerve repair [1][2][3][4]. Additionally, nanofiber scaffolds may be useful in guiding regenerating neurons in the spinal cord and brain, as well as serve to differentiate and guide transplanted neurons and stem cells in nervous system lesions.…”

Section: Introductionmentioning

confidence: 99%

“…Instead of using primary neurons harvested from the nervous system, most in vitro testing of biomaterials relies on the use of renewable cell lines [1,2,[9][10][11][12][13] or, more rarely, primary cells grown in media supplemented with serum [1,3,14,15]. Both of these approaches have significant drawbacks.…”

Section: Introductionmentioning

confidence: 99%

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The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

et al. 2008

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Aligned electrospun nanofibers direct neurite growth and may prove effective for repair throughout the nervous system. Applying nanofiber scaffolds to different nervous system regions will require prior in vitro testing of scaffold designs with specific neuronal and glial cell types. This would be best accomplished using primary neurons in serum-free media; however, such growth on nanofiber substrates has not yet been achieved. Here we report the development of poly(L-lactic acid) (PLLA) nanofiber substrates that support serum-free growth of primary motor and sensory neurons at low plating densities. In our study, we first compared materials used to anchor fibers to glass to keep cells submerged and maintain fiber alignment. We found that poly(lactic-co-glycolic acid) (PLGA) anchors fibers to glass and is less toxic to primary neurons than bandage and glue used in other studies. We then designed a substrate produced by electrospinning PLLA nanofibers directly on cover slips pre-coated with PLGA. This substrate retains fiber alignment even when the fiber bundle detaches from the cover slip and keeps cells in the same focal plane. To see if increasing wettability improves motor neuron survival, some fibers were plasma etched before cell plating. Survival on etched fibers was reduced at the lower plating density. Finally, the alignment of neurons grown on this substrate was equal to nanofiber alignment and surpassed the alignment of neurites from explants tested in a previous study. This substrate should facilitate investigating the behavior of many neuronal types on electrospun fibers in serum-free conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

et al. 2008

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“…Such scaffolds hold the promise to provide the topographic cues to the seeded cells and may potentially enhance tissue regeneration. A few studies have shown that aligned electrospun scaffolds are able to provide contact guidance to cultured cells, resulting in an elongation and alignment of cells along the axes of the fibers [2][3][4]. The majority of the studies revolve around the evaluation of cell morphological changes, whilst some assess the preservation of cell phenotype via gene or protein expression analysis [5,6].…”

Section: Introductionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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Polyhydroxyalkanoate

Bhubalan¹,

Lee²,

Sudesh³

2011

Biodegradable Polymers in Clinical Use and Clinical Development

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Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend

Cited by 668 publications

References 42 publications

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

Polyhydroxyalkanoate

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