Guidance of circular RNAs to proteins’ behavior as binding partners

Luo, Junyun; Liu, Hui; Luan, Siyu; Li, Zhaoyong

doi:10.1007/s00018-019-03216-z

Cited by 47 publications

(28 citation statements)

References 82 publications

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“…Subsequently, we explored whether the function of BCRC-3 was affected by UBAC2, and found that UBAC2 could affect the inhibition of BCRC-3 on the proliferation of BC cells. On the other hand, previous studies have reported that circRNAs can affect the functions of proteins via direct interaction with them 23 – 26 . It has been reported that UBAC2 can negatively regulate the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 53 , and restrict trafficking of UBXD8 from the endoplasmic reticulum to lipid droplets 54 .…”

Section: Discussionmentioning

confidence: 94%

“…To date, numerous functions of circRNAs have been discovered, such as modulating parental gene expression 56 , 57 , regulating alternative splicing 50 , acting as miRNA sponges 20 , 58 , acting as protein sponges or protein scaffolds 26 , 59 , and so on. Among them, a well-defined function of circRNAs is binding with microRNAs (miRNAs) and sequestering them away from their target mRNAs as competitive 23 . In our previous study, we have identified that BCRC-3, circHIPK3, circNR3C1, and circ0001361 can act as “miRNA sponge” to regulate the progress of BC 32 , 36 , 60 , 61 .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a lot of studies have demonstrated that circRNAs play crucial roles in many diseases such as neurological disorders, atherosclerotic vascular disease, carcinomas, and so on 20 – 22 . Recently, increasing numbers of studies indicated that circRNAs could affect the functions of proteins via direct interactions with them 23 . For instance, circRNAs could facilitate their nuclear or cytoplasmic localizations 24 , 25 , regulate their functions or stability 26 , 27 , promote or inhibit the interactions between them 21 , 28 , or influence the interactions between them and DNA by binding directly with proteins 29 , 30 .…”

Section: Introductionmentioning

confidence: 99%

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UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis

Zhao

Zhou

et al. 2020

Cell Death Dis

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Emerging evidences have demonstrated that ubiquitin-associated domain-containing protein 2 (UBAC2) is closely related to the occurrence and development of malignant tumors. However, the functions and underlying molecular mechanisms of UBAC2 in bladder cancer (BC) development have not been defined. In this study, we found that both UBAC2 mRNA and protein levels were upregulated in BC tissues and cell lines, and knockdown of UBAC2 inhibited BC cells proliferation both in vitro and in vivo. Meanwhile, Kaplan–Meier survival plots of 406 BC cases from TCGA database showed that higher expression of UBAC2 in BC patients was associated with lower survival rate. Mechanistic studies revealed that knockdown of UBAC2 increased the expression of p27 by posttranscriptional regulation. Our previous study indicated that circular RNA BCRC-3 (BCRC-3) promoted the expression of p27 through interacting with miR-182-5p, and reversed miR-182-5p-induced inhibition of p27 3′UTR activity. In the present study, we found that UBAC2 could bind to BCRC-3, and subsequently affected the interaction of BCRC-3 with miR-182-5p to inhibit the expression of p27. Furthermore, knockdown of BCRC-3 partly reversed the upregulation of p27 expression induced by knockdown of UBAC2. Our findings highlight a novel mechanism of UBAC2 in regulating p27 through affecting the function of BCRC-3, and provide a research basis for the diagnostic and therapeutic application of BC.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis

Zhao

Zhou

et al. 2020

Cell Death Dis

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“…Circular RNAs (circRNAs) are an emerging class of non-coding RNA (ncRNA) generated by the back splicing of downstream exons to the 3′ acceptor splice site of upstream exons and result in a covalently closed circular structure containing one or more exons [7]. Their mode(s) of action remain to be fully elucidated but they have been suggested to manipulate gene expression by moderation of transcription [8], interaction with cellular proteins [9], sequestration of RNA-binding proteins [10] or sponging miRNA [11]. Their covalently closed structure means that they are resistant to exonucleases; accordingly, they have half-lives on average 19-24 h [12], being significantly more stable than linear mRNAs from their cognate genes which have half-lives typically in the region of 4-9 h [13].…”

Section: Introductionmentioning

confidence: 99%

Islet-expressed circular RNAs are associated with type 2 diabetes status in human primary islets and in peripheral blood

et al. 2020

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Background: Circular RNAs are non-coding RNA molecules with gene regulatory potential that have been associated with several human diseases. They are stable and present in the circulation, making them excellent candidates for biomarkers of disease. Despite their promise as biomarkers or future therapeutic targets, information on their expression and functionality in human pancreatic islets is a relatively unexplored subject. Methods: Here we aimed to produce an enriched circRNAome profile for human pancreatic islets by CircleSeq, and to explore the relationship between circRNA expression, diabetes status, genotype at T2D risk loci and measures of glycaemia (insulin secretory index; SI and HbA1c) in human islet preparations from healthy control donors and donors with type 2 diabetes using ANOVA or linear regression as appropriate. We also assessed the effect of elevated glucose, cytokine and lipid and hypoxia on circRNA expression in the human beta cell line EndoC-βH1. Results: We identified over 2600 circRNAs present in human islets. Of the five most abundant circRNAs in human islets, four (circCIRBP, circZKSCAN, circRPH3AL and circCAMSAP1) demonstrated marked associations with diabetes status. CircCIRBP demonstrated an association with insulin secretory index in isolated human islets and circCIRBP and circRPH3AL displayed altered expression with elevated fatty acid in treated EndoC-βH1 cells. CircCAMSAP1 was also noted to be associated with T2D status in human peripheral blood. No associations between circRNA expression and genotype at T2D risk loci were identified in our samples. Conclusions: Our data suggest that circRNAs are abundantly expressed in human islets, and that some are differentially regulated in the islets of donors with type 2 diabetes. Some islet circRNAs are also expressed in peripheral blood and the expression of one, circCAMSAP1, correlates with diabetes status. These findings highlight the potential of circRNAs as biomarkers for T2D.

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“…Because of their stability, circRNA can accumulate in quiescent and postmitotic cells, such as neurons (Chen and Schuman, 2016), as well as bodily fluids, such as blood, CSF or saliva (Deng et al, 2019;Zhang et al, 2017a;Zhao et al, 2018). circRNAs functions primarily as mi-croRNA (mRNA) or protein sponges (Luo et al, 2019). This circRNA ring structure is thought to be more efficient in suppressing miRNA activity versus linear transcripts (Thomson and Dinger, 2016).…”

Section: Introductionmentioning

confidence: 99%

The differential expression and potential roles of circular RNAs in children with anti-NMDA receptor encephalitis

Guo

Han

Wang

et al. 2020

Journal of Neuroimmunology

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To explore the role of circular RNAs (circRNAs) in pediatric anti-N-methyl-D-aspartate (NMDA) receptor encephalitis, we assessed the profiles of circRNAs and mRNAs in blood leukocytes from anti-NMDA receptor encephalitis patients and healthy controls using microarray analysis. 1196 circRNAs and 719 mRNAs (change ≥2, p < .05) were dysregulated in anti-NMDA receptor encephalitis patients, relative to healthy controls, based on microarray data. Further bioinformatic analysis demonstrated that the host genes of dysregulated circRNAs are primarily associated with receptor internalization. In conclusion, circRNAs may be implicated in the pathogenesis of pediatric anti-NMDA receptor encephalitis.

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Guidance of circular RNAs to proteins’ behavior as binding partners

Cited by 47 publications

References 82 publications

UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis

UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis

Islet-expressed circular RNAs are associated with type 2 diabetes status in human primary islets and in peripheral blood

The differential expression and potential roles of circular RNAs in children with anti-NMDA receptor encephalitis

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