Guidance for formulating ingredients/products from Chlorella vulgaris and Arthrospira platensis considering carotenoid and chlorophyll bioaccessibility and cellular uptake

Nass, Pricila Pinheiro; Nascimento, Tatiele Casagrande do; Fernandes, A. S.; Caetano, Patrícia Acosta; Rosso, Veridiana Vera de; Jacob‐Lopes, Eduardo; Zepka, Leila Queiroz

doi:10.1016/j.foodres.2022.111469

Cited by 12 publications

(3 citation statements)

References 53 publications

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“…They finally enter the circulatory system, becoming accessible to the organism (bioavailability). Many studies have investigated the bioaccessibility of nutrients from microalgae, focusing mainly on phenolic compounds, chlorophylls, and carotenoids [ 7 ]. Far less research has dealt with the bioaccessibility of trace elements, especially iron.…”

Section: Introductionmentioning

confidence: 99%

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Dalmonte

Vecchiato

Biagi

et al. 2023

Veterinary Sciences

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Chlorella vulgaris, Arthrospira platensis, Haematoccocus pluvialis, and Phaeodactylum tricornutum are species of interest for commercial purposes due to their valuable nutritional profile. The aim of this study was to investigate the iron content in these four microalgae, with emphasis on their iron bioaccessibility assessed using an in vitro digestion system to simulate the process which takes place in the stomach and small intestine of dogs, followed by iron quantification using atomic absorption spectrometry. Furthermore, the extraction of soluble proteins was carried out and size exclusion chromatography was applied to investigate iron speciation. Significant differences (p < 0.004) in iron content were found between C. vulgaris, which had the highest (1347 ± 93 μg g−1), and H. pluvialis, which had the lowest (216 ± 59 μg g−1) iron content. C. vulgaris, A. platensis, and H. pluvialis showed an iron bioaccessibility of 30, 31, and 30%, respectively, while P. tricornutum showed the lowest bioaccessibility (11%). The four species analysed presented soluble iron mainly bound to proteins with high molecular mass ranging from >75 to 40 kDa. C. vulgaris showed the highest iron content associated with good bioaccessibility; therefore, it could be considered to be an interesting natural source of organic iron in dog nutrition.

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Section: Introductionmentioning

confidence: 99%

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Dalmonte

Vecchiato

Biagi

et al. 2023

Veterinary Sciences

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“…Chlorella cells are rich in chlorophyll and lutein, which can frequently be utilized as raw materials in producing the natural pigments with high-added value. 6,7 Nevertheless, the process of pigment extraction inevitably produces a large amount of waste Chlorella residue, and developing a reasonable disposal process to deal with these residues and to even collect the remaining resources from them is of great importance. As reported, hightemperature pyrolysis can efficiently convert microalgae to functional biochar toward energy or environmental prospects.…”

Section: Introductionmentioning

confidence: 99%

“…Chlorella cells are rich in chlorophyll and lutein, which can frequently be utilized as raw materials in producing the natural pigments with high-added value. , Nevertheless, the process of pigment extraction inevitably produces a large amount of waste Chlorella residue, and developing a reasonable disposal process to deal with these residues and to even collect the remaining resources from them is of great importance. As reported, high-temperature pyrolysis can efficiently convert microalgae to functional biochar toward energy or environmental prospects. , However, the as-produced microalgal biochar is basically a direct conversion from cells or pigment-extracted residues without the cascade utilization process, and many of the residual components (e.g., proteins and polysaccharides) are lost through high-temperature pyrolysis, which does not meet economic feasibility. , Gratifyingly, owing to the considerable amounts of energy-rich components that still exist in the pigment-extracted microalgal residues, anaerobic microbes can efficiently convert them to CH 4 through a simple operation process with high conversion rate and energy yield, , and this process can be set between pigment extraction and biochar production to achieve the cascade utilization of microalgae.…”

Section: Introductionmentioning

confidence: 99%

Tailored a Multiple Tandem Reaction System for Microalga Cascade Utilization

et al. 2023

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Integrating multiple systems to achieve cascade utilization of microalgae is an advanced biorefinery prospect that can better deal with biowaste disposal. Unfortunately, the current implementations have not explored an efficient and feasible route for Chlorella cascade resource utilization. Here, a multiple tandem reaction system was imaginatively constructed in this investigation for the first time to achieve efficient utilization of Chlorella at different process stages. The system consists of (i) a pigments extraction module, (ii) an anaerobic fermentation module, and (iii) an advanced oxidation processes module. Specifically, the high-valued pigments [chlorophyll (a–b)] were first extracted from Chlorella. Then, the sludge was mixed with pigment-extracted Chlorella residue for anaerobic Co-fermentation to produce methane (CH4). Ultimately, the fermentation residue was calcined at a high temperature and doped with nonmetal (boron)–metal (cobalt) to obtain a recyclable biochar (B,Co-SBC) catalyst. The different modules work in concert with each other to maintain the stable operation of the tandem reaction system. Together, the multiple tandem reaction system proposed in this study can add a new technology library for resource utilization as well as provide a valuable reference for water remediation.

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Nicotine exacerbates liver damage in a mice model of Ehrlich ascites carcinoma through shifting SOD/NF-κB/caspase-3 pathways: ameliorating role of Chlorella vulgaris

Abu-Zeid,

El-Hady,

Ahmed

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Nicotine, a pervasive global environmental pollutant, is released throughout every phase of the tobacco’s life cycle. This study examined the probable ameliorative role of Chlorella vulgaris (ChV) extract against nicotine (NIC)-induced hepatic injury in Ehrlich ascites carcinoma (EAC) bearing female Swiss mice. Sixty female Swiss mice were assigned to four equal groups orally gavaged 2% saccharin 0.2 mL/mouse (control group), orally intubated 100 mg ChV /kg (ChV group), orally intubated 100 µg/mL NIC in 2% saccharin (NIC group), and orally intubated NIC + ChV as in group 3 and 2 (NIC+ChV group). The dosing was daily for 4 weeks. Mice from all experimental groups were then inoculated intraperitoneally with viable tumor cells 2.5 × 106 (0.2 mL/mouse) in the fourth week, and the treatments were extended for another 2 weeks. The results have shown that NIC exposure significantly altered the serum levels of liver function indices, lipid profile, LDH, and ALP in the NIC-exposed group. NIC administration significantly increased hepatic inflammation, lipid peroxidation, and DNA damage-related biomarkers but reduced antioxidant enzyme activities. NIC exposure downregulated SOD1, SOD2, CAT, GPX1, and GPX2 but upregulated NF-κB hepatic gene expression. Notably, the presence of the EAC cells outside the liver was common in all mice groups. Liver tissue of the NIC-exposed group showed multifocal expansion of hepatic sinusoids by neoplastic cells. However, with no evidence of considerable infiltration of EAC cells inside the sinusoids or in periportal areas in the NIC + ChV groups. NIC significantly altered caspase-3, Bax, and BcL2 hepatic immune expression. Interestingly, ChV administration significantly mitigates NIC-induced alterations in hepatic function indices, lipid profile, and the mRNA expression of antioxidant and NF-κB genes and regulates the caspase-3, Bax, and BcL2 immunostaining. Finally, the in vivo protective outcomes of ChV against NIC-induced hepatic injury combined with EAC in female Swiss mice could suggest their helpful role for cancer patients who are directly or indirectly exposed to NIC daily.

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Guidance for formulating ingredients/products from Chlorella vulgaris and Arthrospira platensis considering carotenoid and chlorophyll bioaccessibility and cellular uptake

Cited by 12 publications

References 53 publications

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Tailored a Multiple Tandem Reaction System for Microalga Cascade Utilization

Nicotine exacerbates liver damage in a mice model of Ehrlich ascites carcinoma through shifting SOD/NF-κB/caspase-3 pathways: ameliorating role of Chlorella vulgaris

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