Guidance-Cue Control of Horizontal Cell Morphology, Lamination, and Synapse Formation in the Mammalian Outer Retina

Matsuoka, Ryota; Jiang, Zheng; Samuels, Ivy S.; Nguyen-Ba-Charvet, Kim T.; Sun, Lu; Peachey, Neal S.; Chédotal, Alain; Yau, King Wai; Kolodkin, Alex L.

doi:10.1523/jneurosci.0267-12.2012

Cited by 62 publications

(61 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have identified multiple repulsive signaling pathways that function in dendrite patterning of vertebrate and invertebrate neurons, including semaphorin/plexin (Polleux et al 2000;Matsuoka et al 2012;Sun et al 2013), Dscams (Matthews et al 2007;Soba et al 2007;Fuerst et al 2009), protocadherins (Lefebvre et al 2012), Netrin/DCC/UNC-5 (Smith et al 2012), and Slit/Robo (Furrer et al 2007;Gibson et al 2014). We thus examined potential roles of the repulsive factors in the ventral boundary specification of v'ada dendrites by using genetic mutants or RNAi knockdown, but, to our surprise, none of them showed obvious defects in v'ada dendrite boundary specification (Supplemental Table S1).…”

Section: Discussionmentioning

confidence: 99%

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

et al. 2015

View full text Add to dashboard Cite

Sensory neurons with common functions are often nonrandomly arranged and form dendritic territories in stereotypic spatial patterns throughout the nervous system, yet molecular mechanisms of how neurons specify dendritic territories remain largely unknown. In Drosophila larvae, dendrites of class IV sensory (C4da) neurons completely but nonredundantly cover the whole epidermis, and the boundaries of these tiled dendritic fields are specified through repulsive interactions between homotypic dendrites. Here we report that, unlike the larval C4da neurons, adult C4da neurons rely on both dendritic repulsive interactions and external positional cues to delimit the boundaries of their dendritic fields. We identify Wnt5 derived from sternites, the ventral-most part of the adult abdominal epidermis, as the critical determinant for the ventral boundaries. Further genetic data indicate that Wnt5 promotes dendrite termination on the periphery of sternites through the Ryk receptor family kinase Derailed (Drl) and the Rho GTPase guanine nucleotide exchange factor Trio in C4da neurons. Our findings thus uncover the dendritic contact-independent mechanism that is required for dendritic boundary specification and suggest that combinatory actions of the dendritic contact-dependent and -independent mechanisms may ensure appropriate dendritic territories of a given neuron.

show abstract

Section: Discussionmentioning

confidence: 99%

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Loss of Dscam or Dscaml1 function results in a soma and dendrite-clustering phenotype that superficially resembles a similar dendrite self-avoidance phenotype observed in Drosophila Dscam1 mutants (Zhu et al, 2006;Matthews et al, 2007). Dscam1 has been shown to actively drive avoidance through repulsion, which limits dendrites of a given cell from crossing other dendrites originating from the same cell (Hughes et al, 2007).…”

Section: Dscam and Dendrite Avoidancementioning

confidence: 93%

“…4). These results suggest that the role of mouse Dscam may be better reflected by some of the other Drosophila Dscams or phenotypes (Millard et al, 2010;Hutchinson et al, 2014), with the role of Drosophila Dscam1 in mouse likely provided by other molecules, such as the ␥-protocadherins (Lefebvre et al, 2008(Lefebvre et al, , 2012.…”

Section: Dscam and Dendrite Avoidancementioning

confidence: 99%

“…We generated a transgenic mouse model that conditionally expresses Dscam under control of the constitutive CAG promotor (Miyazaki et al, 1989). This allele, denoted as Dscam floxGOF before Cre-mediated recombination, expresses either RFP, or Dscam and GFP, after Cre-mediated recombination removes the RFP sequence (Fig.…”

Section: Generation Of a Conditional Dscam Gain-of-function Mouse Modelmentioning

confidence: 99%

See 1 more Smart Citation

DSCAM Promotes Refinement in the Mouse Retina through Cell Death and Restriction of Exploring Dendrites

Sukeena

Simmons

et al. 2015

J. Neurosci.

Self Cite

View full text Add to dashboard Cite

In this study we develop and use a gain-of-function mouse allele of the Down syndrome cell adhesion molecule (Dscam) to complement loss-of-function models. We assay the role of Dscam in promoting cell death, spacing, and laminar targeting of neurons in the developing mouse retina. We find that ectopic or overexpression of Dscam is sufficient to drive cell death. Gain-of-function studies indicate that Dscam is not sufficient to increase spatial organization, prevent cell-to-cell pairing, or promote active avoidance in the mouse retina, despite the similarity of the Dscam loss-of-function phenotype in the mouse retina to phenotypes observed in Drosophila Dscam1 mutants. Both gain-and loss-of-function studies support a role for Dscam in targeting neurites; DSCAM is necessary for precise dendrite lamination, and is sufficient to retarget neurites of outer retinal cells after ectopic expression. We further demonstrate that DSCAM guides dendrite targeting in type 2 dopaminergic amacrine cells, by restricting the stratum in which exploring retinal dendrites stabilize, in a Dscam dosage-dependent manner. Based on these results we propose a single model to account for the numerous Dscam gain-and loss-of-function phenotypes reported in the mouse retina whereby DSCAM eliminates inappropriately placed cells and connections.

show abstract

“…Nonetheless, new concepts in this area are emerging quickly, including the demonstration of molecular control over outer retinal cell type tiling by guidance cue signaling. Kolodkin and coworkers (Matsuoka et al 2012) recently showed that the repellant guidance cues Sema6a and PlexinA4 are important for self-avoidance of horizontal cell processes. The independence of such phenotypes between specific retinal neuron subtypes and their expression of distinct ligands, receptors, and isoform profiles reinforces the idea that while mosaic spacing and tiling are ubiquitous features in retinal neurons, each retinal neuron subtype may employ a different set of molecular signals to achieve these key features of retinal organization.…”

Section: Cell Type Specification In the Mammalian Visual Systemmentioning

confidence: 99%

So many pieces, one puzzle: cell type specification and visual circuitry in flies and mice

2014

View full text Add to dashboard Cite

The visual system is a powerful model for probing the development, connectivity, and function of neural circuits. Two genetically tractable species, mice and flies, are together providing a great deal of understanding of these processes. Current efforts focus on integrating knowledge gained from three cross-fostering fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types (''connectomics'') by high-resolution three-dimensional circuit anatomy, and (3) causal testing of how identified circuit elements contribute to visual perception and behavior. Here we discuss representative examples from fly and mouse models to illustrate the ongoing success of this tripartite strategy, focusing on the ways it is enhancing our understanding of visual processing and other sensory systems.For many decades, the visual systems of both vertebrates and invertebrates have been a favorite arena for understanding how neural circuits are built and function. A considerable body of work has focused on the specification of cell types of the retina; for instance, the designation of different classes of photoreceptors with distinct spectral sensitivities in the Drosophila retina (Rister and Desplan 2011) and the establishment of the various cell types that comprise the vertebrate retina: photoreceptors, Muller glia, interneurons (horizontal, amacrine, and bipolar cells), and retinal ganglion cells (RGCs) (Livesey and Cepko 2001;Mu et al. 2004;Poch e and Reese 2006). With that knowledge in hand, focus in recent years has expanded to understanding how the circuits formed by these retinal cells are linked to the staggering number of diverse visual neurons in the brain, which in turn enables sophisticated and diverse computational tasks. The ultimate goal is to understand how cellular identity, function, and connectivity relate to visually guided behaviors. (Fig. 1A).

show abstract

Guidance-Cue Control of Horizontal Cell Morphology, Lamination, and Synapse Formation in the Mammalian Outer Retina

Cited by 62 publications

References 48 publications

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

DSCAM Promotes Refinement in the Mouse Retina through Cell Death and Restriction of Exploring Dendrites

So many pieces, one puzzle: cell type specification and visual circuitry in flies and mice

Contact Info

Product

Resources

About