Guests involved CB[8] capped silver nanoparticles as a means of electrochemical signal enhancement for sensitive detection of Caspase-3

Song, Sunfengda; Hu, Xiaojun; Li, Hongjie; Zhao, Jialin; Koh, Kwangnak; Chen, Hongxia

doi:10.1016/j.snb.2018.07.143

Cited by 21 publications

(4 citation statements)

References 32 publications

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“…The value was lower than those obtained by the homogeneous assays, including fluorescence (0.128 ng/ mL), 34 colorimetry (5 ng/mL), 40 electrochemistry (27.4 ng/ mL), 41 and mass spectrometry (3.02 ng/mL). 26 The detection limit is comparable to or even lower than that achieved by the heterogeneous electrochemical analysis with the signal amplification of enzymes or nanomaterials, such as methylene blue/graphene oxide (0.06 pg/mL), 42 calixarene-functionalized reduced graphene oxide (0.0167 pg/mL), 43 citratestabilized (0.1 pg/mL) or CB [8]-capped (24.62 pg/mL) silver nanoparticles, 44,45 magnetic bead/horseradish peroxidase (MB-HRP) (27.4 ng/mL), 46 and gold nanoparticle-coated silica-based mesoporous materials (AuNPs-MCM)/MB-HRP (1.37 pg/mL). 47 The high sensitivity can be attributed to the poor conductivity of (SA−biotin−GDEVDGK−biotin) n ag-gregates and the competition reaction between the hydrolysis products (biotin−GDEVD or GK−biotin) and the peptide substrate to bind SA both on the electrode surface and in solution.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

et al. 2021

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This work proposed a new sensing strategy for protease detection by converting a homogeneous assay into a surface-tethered electrochemical analysis. Streptavidin (SA), a tetramer protein, was used as the sensing unit based on the SA–biotin coupling chemistry. Caspase-3 was used as the model analyte, and a biotinylated peptide with a sequence of biotin–GDEVDGK–biotin was designed as the substrate. Specifically, the peptide substrate could induce an assembly of SA to form (SA–biotin–GDEVDGK–biotin) n aggregates through SA–biotin interactions, which was confirmed by atomic force microscopy (AFM). The peptide substrate-induced assembly of SA was facilely initiated on an electrode–liquid surface by modification of the electrode with SA. The in situ formation of (SA–biotin–GDEVDGK–biotin) n aggregates created an insulating layer, thus limiting the electron transfer of ferricyanide. Once the peptide substrate was cleaved into two shorter fragments (biotin–GDEVD and GK–biotin) by caspase-3, the resulting products would compete with biotin–GDEVDGK–biotin to bind SA proteins immobilized on the electrode surface and distributed in a solution, thus preventing the in situ formation of (SA–biotin–GDEVDGK–biotin) n assemblies. With the simple principle of the substrate-induced assembly of SA, a dual-signal amplification was achieved with improved sensitivity. Taking advantage of high sensitivity, simple principle, and easy operation, this method can be augmented to design various surface-tethered biosensors for practical applications.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

et al. 2021

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“…3 , b). Aggregates were specifically manufactured, for example, by reaction with CB[8] [ 91 ], DNA [ 92 ], or peptide [ 93 ] templated synthesis. However, analogous to the SPR aggregation sensor, finely tuned surfaces and methods are of immense importance as the signals vary greatly upon unspecific or uncontrolled aggregation, which typically occur in biological matrices.…”

Section: Silver Nanoparticles In Electrochemical Biosensorsmentioning

confidence: 99%

Signaling strategies of silver nanoparticles in optical and electrochemical biosensors: considering their potential for the point-of-care

2023

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Silver nanoparticles (AgNPs) have long been overshadowed by gold NPs’ success in sensor and point-of-care (POC) applications. However, their unique physical, (electro)chemical, and optical properties make them excellently suited for such use, as long as their inherent higher instability toward oxidation is controlled. Recent advances in this field provide novel strategies that demonstrate that the AgNPs’ inherent capabilities improve sensor performance and enable the specific detection of analytes at low concentrations. We provide an overview of these advances by focusing on the nanosized Ag (in the range of 1-100 nm) properties with emphasis on optical and electrochemical biosensors. Furthermore, we critically assess their potential for point-of-care sensors discussing advantages as well as limitations for each detection technique. We can conclude that, indeed, strategies using AgNP are ready for sensitive POC applications; however, research focusing on the simplification of assay procedures is direly needed for AgNPs to make the successful jump into actual applications. Graphical abstract

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“…Song et al dispersed Ag nanoparticles (NPs) onto bare gold electrode by wet chemistry approach, to amplify electrochemical signals of cysteine aspartase protease (Caspase‐3, Figure 1 A). [ 22 ] The assembly of Ag NPs not only prevented the conventional agglomeration but also enhanced the electrochemical signals by electron transfer between electrode and silver redox center. As a result, the detection of limit (LOD) for Caspase‐3 was 24.62 pg mL −1 , which was 65.75‐fold lower compared to the classic absorption method.…”

Section: Types Of Nanomaterial‐based Electrochemical Sensorsmentioning

confidence: 99%

Section: Types Of Nanomaterial‐based Electrochemical Sensorsmentioning

confidence: 99%

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

Liu

Huang

Qian

2021

Advanced NanoBiomed Research

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Nanomaterials possess unique properties, including excellent electrochemical properties, high surface area, and tunable electric conductivity, making them attractive for sensing applications, especially for electrochemical sensing. Notably, the compatibility of nanomaterials with other techniques opens up opportunities for electrochemical sensors in various biomedical applications. Herein, the enhancing mechanisms and preparation protocols of electrochemical sensors, regarding the material choices, are introduced. The integration of nanomaterial‐based electrochemical sensors with other techniques toward precise health management and controlled drug release is further highlighted. It is concluded with perspectives on the future directions for this topic. Future research directions of nanomaterial‐based electrochemical sensors and the challenges faced by commercialized implementation of the sensors are presented, paving the way for the upcoming era of accurate biosensing toward both academic and industrial use.

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Guests involved CB[8] capped silver nanoparticles as a means of electrochemical signal enhancement for sensitive detection of Caspase-3

Cited by 21 publications

References 32 publications

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

Signaling strategies of silver nanoparticles in optical and electrochemical biosensors: considering their potential for the point-of-care

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

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