Guard cell regulation: pulling the strings behind the scenes

Siqueira, João Antônio; Oliveira, Hellen Oliveira de; Nunes‐Nesi, Adriano; Araújo, Wagner L.

doi:10.1016/j.tplants.2021.07.005

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Therefore, while Glu and Gln seems to be important sinks of the carbons derived from CO 2 assimilation mediated by both RuBisCO and PEPc, the metabolic flux from Glu/Gln to GABA may be restricted during the dark-to-light transition as a mechanism to allow stomatal opening. This idea is supported by the fact that GABA is a negative regulator of ALTM9 (aluminium-activated malate transporter 9) (Xu et al 2021;Siqueira et al 2021), a key vacuolar anion uptake channel activated during stomatal opening (De Angeli et al 2013;Medeiros et al 2018a).…”

Section: Regulation Of the Tca Cycle And Associated Metabolic Pathway...mentioning

confidence: 99%

PEPc-mediated CO2 assimilation provides carbons to gluconeogenesis and the TCA cycle in both dark-exposed and illuminated guard cells

Lima,

Medeiros,

Cândido‐Sobrinho

et al. 2024

Preprint

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Evidence suggests that guard cells have higher rate of phosphoenolpyruvate carboxylase (PEPc)-mediated dark CO2 assimilation than mesophyll cells. However, it is unknown which metabolic pathways are activated following dark CO2 assimilation in guard cells. Furthermore, it remains unclear how the metabolic fluxes throughout the tricarboxylic acid (TCA) cycle and associated pathways are regulated in illuminated guard cells. Here we used 13C-HCO3 labelling of tobacco guard cells harvested under continuous dark or during the dark-to-light transition to elucidate principles of metabolic dynamics downstream of CO2 assimilation. Most metabolic changes were similar between dark-exposed and illuminated guard cells. However, illumination increased the 13C-enrichment in sugars and metabolites associated to the TCA cycle. Sucrose was labelled in the dark, but light exposure increased the 13C-labelling into this metabolite. Fumarate was strongly labelled under both dark and light conditions, while illumination increased the 13C-enrichment in pyruvate, succinate and glutamate. Only one 13C was incorporated into malate and citrate in either dark or light conditions. Our results collectively suggest that the PEPc-mediated CO2 assimilation provides carbons for gluconeogenesis, the TCA cycle and glutamate synthesis and that previously stored malate and citrate are used to underpin the specific metabolic requirements of illuminated guard cells.

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Section: Regulation Of the Tca Cycle And Associated Metabolic Pathway...mentioning

confidence: 99%

PEPc-mediated CO2 assimilation provides carbons to gluconeogenesis and the TCA cycle in both dark-exposed and illuminated guard cells

Lima,

Medeiros,

Cândido‐Sobrinho

et al. 2024

Preprint

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“…The precise regulation of stomatal movement is critical for plant adaptation to drought stress [ 176 ]. The disassembly of MTs is critical for stomatal closure in response to abscisic acid (ABA) and drought stress [ 177 ].…”

Section: Mts In Abiotic Stress Responsesmentioning

confidence: 99%

Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation

Hsiao

Huang

2023

Biomolecules

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Microtubules (MTs) are essential elements of the eukaryotic cytoskeleton and are critical for various cell functions. During cell division, plant MTs form highly ordered structures, and cortical MTs guide the cell wall cellulose patterns and thus control cell size and shape. Both are important for morphological development and for adjusting plant growth and plasticity under environmental challenges for stress adaptation. Various MT regulators control the dynamics and organization of MTs in diverse cellular processes and response to developmental and environmental cues. This article summarizes the recent progress in plant MT studies from morphological development to stress responses, discusses the latest techniques applied, and encourages more research into plant MT regulation.

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“…During plant growth, several factors can influence the features of stomata, including hormones, light conditions, and atmospheric CO 2 concentration [ 6 , 7 ]. Stomata density and size tend to increase with higher light intensity and decrease with increasing CO 2 concentration [ 8 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Rotating Stomata Measurement Based on Anchor-Free Object Detection and Stomata Conductance Calculation

Zhang,

Wang,

et al. 2023

Plant Phenomics

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Stomata play an essential role in regulating water and carbon dioxide levels in plant leaves, which is important for photosynthesis. Previous deep learning-based plant stomata detection methods are based on horizontal detection. The detection anchor boxes of deep learning model are horizontal, while the angle of stomata is randomized, so it is not possible to calculate stomata traits directly from the detection anchor boxes. Additional processing of image (e.g., rotating image) is required before detecting stomata and calculating stomata traits. This paper proposes a novel approach, named DeepRSD (deep learning-based rotating stomata detection), for detecting rotating stomata and calculating stomata basic traits at the same time. Simultaneously, the stomata conductance loss function is introduced in the DeepRSD model training, which improves the efficiency of stomata detection and conductance calculation. The experimental results demonstrate that the DeepRSD model reaches 94.3% recognition accuracy for stomata of maize leaf. The proposed method can help researchers conduct large-scale studies on stomata morphology, structure, and stomata conductance models.

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Guard cell regulation: pulling the strings behind the scenes

Cited by 8 publications

References 12 publications

PEPc-mediated CO2 assimilation provides carbons to gluconeogenesis and the TCA cycle in both dark-exposed and illuminated guard cells

PEPc-mediated CO2 assimilation provides carbons to gluconeogenesis and the TCA cycle in both dark-exposed and illuminated guard cells

Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation

Rotating Stomata Measurement Based on Anchor-Free Object Detection and Stomata Conductance Calculation

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