Guanosine 3',5'-Cyclic Monophosphate Reduces the Response of the Moth's Olfactory Receptor Neuron to Pheromone

Redkozubov, Alexei

doi:10.1093/chemse/25.4.381

Cited by 22 publications

(17 citation statements)

References 27 publications

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“…Daytime dependent olfactory adaptation by cGMP and antennal homogenates of Antherea polyphemus and Bombyx mori, remaining elevated for nearly 30·min and, thus, matching the time course of long-term adaptation (Ziegelberger et al, 1990;Boekhoff et al, 1993). In the silkmoth Bombyx mori the perfusion of the sensillar lymph with dibutyryl guanosine 3Ј,5Ј-cyclic monophosphate caused a suppression of the sensillar potential and a reduction of the AP response in pheromone-dependent sensilla, mimicking a state of adaptation (Redkozubov, 2000).…”

Section: Introductionmentioning

confidence: 66%

“…This selective decrease in the AP frequency distinguishes one of the different forms of adaptation in Manduca sexta for the first time. Interestingly, injection of dibutyryl guanosine 3Ј,5Ј-cyclic monophosphate into the antennae of Bombyx mori decreased the AP frequency as well as the sensillar potential amplitude (Redkozubov, 2000). The recording methods and the cGMP analogues employed differed between both moth species, as well as the stimulation scheme.…”

Section: Discussionmentioning

confidence: 99%

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Perfusion with cGMP analogue adapts the action potential response of pheromone-sensitive sensilla trichoidea of the hawkmoth Manduca sextain a daytime-dependent manner

Flecke

Dolzer

Krannich

et al. 2006

Journal of Experimental Biology

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SUMMARY Pheromone-dependent mate search is under strict circadian control in different moth species. But it remains unknown whether daytime-dependent changes in pheromone sensitivity already occur at the periphery in male moths. Because adapting pheromone stimuli cause rises of cyclic guanosine monophosphate (cGMP) in pheromone-sensitive trichoid sensilla of the night-active hawkmoth Manduca sexta, we wanted to determine whether cGMP decreases pheromone-sensitivity of olfactory receptor neurons in a daytime-dependent manner. Long-term tip recordings from trichoid sensilla were performed at the early day (ZT 1-4), when many moths are still active, and at the middle of the day (ZT 8-11), when moths are resting. A non-adapting pheromone-stimulation protocol combined with perfusion of the sensillum lymph with the membrane-permeable cGMP analogue 8bcGMP adapted the action potential response but not the sensillar potential. Perfusion with 8bcGMP decreased the initial action potential frequency, decreased the numbers of action potentials elicited in the first 100 ms of the pheromone response and attenuated the reduction of action potential amplitude. Furthermore, the decrease in 8bcGMP-dependent action potential frequency was stronger in recordings made at ZT 8-11 than at ZT 1-4. In the control recordings during the course of the day the pheromone responses became increasingly tonic and less phasic. At ZT 8-11 only, this daytime-dependent effect was further enhanced by 8bcGMP application. Thus we hypothesize that during the moths' resting phase,elevated cGMP levels underlie a daytime-dependent decrease in pheromone sensitivity and a decline in the temporal resolution of pheromone pulses.

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Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Perfusion with cGMP analogue adapts the action potential response of pheromone-sensitive sensilla trichoidea of the hawkmoth Manduca sextain a daytime-dependent manner

Flecke

Dolzer

Krannich

et al. 2006

Journal of Experimental Biology

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“…Application of the membrane-permeable dbcGMP to the outer dendritic membrane of the moth's olfactory receptor neuron reduced its response to pheromones (Redkozubov, 2000). The observed attenuated response was attributed to the reduction of an elementary receptor current, which elicits nerve impulses and underlies the overall receptor current.…”

Section: Discussionmentioning

confidence: 96%

Decrease in DEET repellency caused by nitric oxide in Rhodnius prolixus

Sfara

Zerba

Alzogaray

2007

Arch Insect Biochem Physiol

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N,N-diethyl-3-methylbenzamide (DEET) is widely used as an insect repellent; however, little is known about its mode of action. On the other hand, nitric oxide (NO) participates in the olfaction transduction pathway of insects. In this work, nitroso-acetyl-cysteine (SNAC), a nitric oxide donor, or dibutyril-cyclic-GMP (db-cGMP), the cyclic nucleotide analog, were applied on fifth instar nymphs of Rhodnius prolixus before exposing them to DEET, to obtain information about the possible role of NO/cGMP system in the olfaction process. In the first place, we exposed the nymphs to several DEET concentrations (70, 700, 1,750, and 3,500 microg/cm2). All these concentrations produced a repellent effect. A decrease in repellency during the course of the experiment was observed when the nymphs were exposed to high concentrations of DEET (700 and 1,750 microg/cm2), suggesting an adaptation phenomenon. The pre-treatment of the insects with 15 microg /insect of SNAC or 2 microg/insect of db-cGMP produced a reduction of the repellency. An increase in locomotor activity was observed in insects exposed to 350 or 700 microg/cm2 DEET. Although exposure to 70 microg/cm2 DEET produced a high repellency response, it did not modify the insects' locomotor activity. Insects treated with two doses of SNAC before being exposed to 350 microg/cm2 of DEET showed no differences in locomotor activity compared to controls.

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“…Most studies on NO and the sensorial function in insects have used biochemical (Stengl & Zintl, 1996;Redkozubov, 2000;Newland & Yates, 2008) or electrophysiological (Stengl et al, 2001;Waserman & Itagaki, 2003;Dolzer et al, 2008) methods. However, the insect's behavioural response was only studied in a few cases, mainly in the locust (Newland & Yates, 2007;Wenzel et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Deterrence of feeding in Rhodnius prolixus (Hemiptera: Reduviidae) after treatment of antennae with a nitric oxide donor

Sfara¹,

Zerba²,

Alzogaray³

2011

Eur. J. Entomol.

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Abstract. The blood-sucking bug Rhodnius prolixus is the main vector of Chagas Disease in Colombia, Venezuela and several countries in Central America. Nitric oxide (NO) is a ubiquitous gaseous molecule present in most types of cell and participates in the olfactory pathway of insects. In this work, nitroso-acetyl-cysteine (SNAC), a nitric oxide donor, was topically applied to the antennae of fifth instar nymphs of R. prolixus. After SNAC treatment, these insects showed a dose-dependent reluctance to feed when provided with a living pigeon as the food source (ED50 = 5.2 µg/insect). However, there was no reluctance to feed when dbcGMP was applied to the antennae of nymphs. In another experiment, insects that had their antennae treated with SNAC were less attracted than the control group to a CO2 source. A possible role of NO in the olfactory pathway of R. prolixus is discussed.

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Guanosine 3',5'-Cyclic Monophosphate Reduces the Response of the Moth's Olfactory Receptor Neuron to Pheromone

Cited by 22 publications

References 27 publications

Perfusion with cGMP analogue adapts the action potential response of pheromone-sensitive sensilla trichoidea of the hawkmoth Manduca sextain a daytime-dependent manner

Perfusion with cGMP analogue adapts the action potential response of pheromone-sensitive sensilla trichoidea of the hawkmoth Manduca sextain a daytime-dependent manner

Decrease in DEET repellency caused by nitric oxide in Rhodnius prolixus

Deterrence of feeding in Rhodnius prolixus (Hemiptera: Reduviidae) after treatment of antennae with a nitric oxide donor

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