Guanine tautomerism revealed by UV–UV and IR–UV hole burning spectroscopy

Nir, Eyal; Janzen, Christoph; Imhof, Petra; Kleinermanns, Karl; Vries, Mattanjah S. de

doi:10.1063/1.1391443

Cited by 193 publications

(278 citation statements)

References 25 publications

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“…Numbers are relative energies in cm -1 . Panels (e), (g) and (h) show experimental IR-UV double resonance spectra from Nir et al (31). The labels A-D refer to the four tautomers observed in REMPI spectroscopy.…”

Section: Monomers and Tautomeric Formsmentioning

confidence: 99%

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Gas-Phase IR Spectroscopy of Nucleobases

Vries

2014

Topics in Current Chemistry

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Abstract.IR spectroscopy of nucleobases in the gas phase reflects simultaneous advances in both experimental and computational technique. Important properties, such as excited state dynamics, depend in subtle ways on structure variations, which can be followed by their infrared signatures. Isomer specific spectroscopy is a particularly powerful tool for studying the effects of nucleobase tautomeric form and base pair hydrogen bonding patterns.

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Section: Monomers and Tautomeric Formsmentioning

confidence: 99%

“…Nir et al obtained IR-UV DRS spectra of a series of guanosines, some of which appear in Figure 2 (31). Trace (a) shows 9H enol guanine (trace (c) in Figure 2) for comparison.…”

Section: Purinesmentioning

confidence: 99%

Gas-Phase IR Spectroscopy of Nucleobases

Vries

2014

Topics in Current Chemistry

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“…This journal is © The Royal Society of Chemistry 2012 stable conformers in the gas phase, [52][53][54] 62 although an earlier simulation suggested that the C 6 pucker CI may also be involved. 65 In the solution phase, Gua itself is rarely studied as it has poor solubility, although dGMP -has been studied in a number of experiments with transient absorption 1,16,66 and fluorescence upconversion.…”

Section: Dgmp -mentioning

confidence: 99%

Time-resolved photoelectron imaging of the isolated deprotonated nucleotides

et al. 2014

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Publisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. Using time-resolved photoelectron spectroscopy, the excited state dynamics of gas-phase mass-selected nucleotide anions have been monitored following UV excitation at 4.66 eV. The spectra reveal that the dynamics of the 2'-deoxyguanosine 5'-monophosphate anion (dGMP -) are very similar to those of the adenosine nucleotide (dAMP -) and insensitive to a solvation environment. Comparison of our results with other literature suggest s that nucleotides of the two purine bases share a common relaxation pathway, whereby the initially populated 1 ππ* states relax to the ground electronic state without involvement of any other intermediary electronic states. In the analogous pyrimidine nucleotides of thymine and cytosine, dTMP -and dCMP -, no such unified mechanism is observed. Photoexcited dTMP -behaves much like the isolated nucleobase thymine, exhibiting rapid relaxation to the ground electronic state, although with a minor long-lived channel. On the other hand, isolated dCMP -is longer lived than its cytosine nucleobase, and hence it appears that the presence of the sugar and phosphate in the nucleotide arrangement leads to a modification of the available relaxation pathways. Nucleotides are the basic monomer building blocks of DNA and our results present important new benchmark data to develop an understanding of the molecular mechanism by which photodamage can be mediated when DNA is exposed to UV light.

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“…Traditionally, resonant two-photon ionization (R2PI) and optical double resonance spectroscopies [5][6][7][8][9] have been used to study the guanine tautomers produced via laser desorption. However, Choi and Miller 10 showed that the tautomer distribution observed by IR spectroscopy of guanine formed by thermal vaporization with subsequent entrainment in ultracold helium droplets differs from that observed in laser desorption probed by R2PI.…”

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confidence: 99%

Experimental Observation of Guanine Tautomers with VUV Photoionization

et al. 2009

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Two methods of preparing guanine in the gas phase, thermal vaporization and laser desorption, have been investigated. The guanine generated by each method is entrained in a molecular beam, single-photon ionized with tunable VUV synchrotron radiation, and analyzed using reflectron mass spectrometry. The recorded photoionization efficiency (PIE) curves show a dramatic difference for experiments performed via thermal vaporization compared to that with laser desorption. The calculated vertical and adiabatic ionization energies for the eight lowest-lying tautomers of guanine suggest that the experimental observations arise from different tautomers being populated in the two different experimental methods.

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Guanine tautomerism revealed by UV–UV and IR–UV hole burning spectroscopy

Cited by 193 publications

References 25 publications

Gas-Phase IR Spectroscopy of Nucleobases

Gas-Phase IR Spectroscopy of Nucleobases

Time-resolved photoelectron imaging of the isolated deprotonated nucleotides

Experimental Observation of Guanine Tautomers with VUV Photoionization

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