GSZ Formula Enhances Sleep Quality: Exploring Its Active Ingredients and Mechanism Using a Network Medicine Approach

Ren, Airong; Ma, Mingxuan; Liang, Yongyin; Wang, Yarong; Li, Zhengkun; Liu, Yahui; Fan, Qing; Cui, Guozhen

doi:10.1016/j.ccmp.2023.100107

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…To construct a network involving drug targets and disease‐related proteins, we first mapped the ingredient target proteins and ALD proteins to the human interaction network. Subsequently, we extracted a sub‐network to generate the drug–disease subgraph utilizing the Python NetworkX package, as previously described (Airong Ren et al., 2024 ; Ren et al., 2023 ). The extracted node and edge lists were subsequently imported into Gephi software (version 0.9.2) to generate a comprehensive drug–disease diagram.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the samples were centrifuged at 4000× g for 20 min. The supernatant was characterized by a Waters Ultra‐High‐Performance Liquid Chromatography‐Quadrupole/Time‐of‐Flight–Tandem Mass Spectrometry (UPLC‐Q/TOF–MS/MS), as previously described (Ren et al., 2024 ; Ren et al., 2023 ). Chromatographic separation was performed on a Waters Acquity™ UPLC system using an ACQUITY UPLC BEH C18 column.…”

Section: Methodsmentioning

confidence: 99%

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Network medicine‐based analysis of the hepatoprotective effects of Amomum villosum Lour. on alcoholic liver disease in rats

Wei,

Wang,

Huang

et al. 2024

Food Science & Nutrition

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Alcoholic liver disease (ALD) is characterized by high morbidity and mortality, and mainly results from prolonged and excessive alcohol use. Amomum villosum Lour. (A. villosum), a well‐known traditional Chinese medicine (TCM), has hepatoprotective properties. However, its ability to combat alcohol‐induced liver injury has not been fully explored. The objective of this study was to investigate the hepatoprotective effects of A. villosum in a rat model of alcohol‐induced liver disease, thereby establishing a scientific foundation for the potential preventive use of A. villosum in ALD. We established a Chinese liquor (Baijiu)‐induced liver injury model in rats. Hematoxylin and eosin (HE) staining, in combination with biochemical tests, was used to evaluate the protective effects of A. villosum on the liver. The integration of network medicine analysis with experimental validation was used to explore the hepatoprotective effects and potential mechanisms of A. villosum in rats. Our findings showed that A. villosum ameliorated alcohol‐induced changes in body weight, liver index, hepatic steatosis, inflammation, blood lipid metabolism, and liver function in rats. Network proximity analysis was employed to identify 18 potentially active ingredients of A. villosum for ALD treatment. These potentially active ingredients in the blood were further identified using mass spectrometry (MS). Our results showed that A. villosum plays a hepatoprotective role by modulating the protein levels of estrogen receptor 1 (ESR1), anti‐nuclear receptor subfamily 3 group C member 1 (NR3C1), interleukin 6 (IL‐6), and tumor necrosis factor‐α (TNF‐α). In conclusion, the results of the current study suggested that A. villosum potentially exerts hepatoprotective effects on ALD in rats, possibly through regulating the protein levels of ESR1, NR3C1, IL‐6, and TNF‐α.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Network medicine‐based analysis of the hepatoprotective effects of Amomum villosum Lour. on alcoholic liver disease in rats

Wei,

Wang,

Huang

et al. 2024

Food Science & Nutrition

Self Cite

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Network Medicine: A Potential Approach for Virtual Drug Screening

Ma,

Huang,

et al. 2024

Pharmaceuticals

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Traditional drug screening methods typically focus on a single protein target and exhibit limited efficiency due to the multifactorial nature of most diseases, which result from disturbances within complex networks of protein–protein interactions rather than single gene abnormalities. Addressing this limitation requires a comprehensive drug screening strategy. Network medicine is rooted in systems biology and provides a comprehensive framework for understanding disease mechanisms, prevention, and therapeutic innovations. This approach not only explores the associations between various diseases but also quantifies the relationships between disease genes and drug targets within interactome networks, thus facilitating the prediction of drug–disease relationships and enabling the screening of therapeutic drugs for specific complex diseases. An increasing body of research supports the efficiency and utility of network-based strategies in drug screening. This review highlights the transformative potential of network medicine in virtual therapeutic screening for complex diseases, offering novel insights and a robust foundation for future drug discovery endeavors.

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GSZ Formula Enhances Sleep Quality: Exploring Its Active Ingredients and Mechanism Using a Network Medicine Approach

Cited by 2 publications

References 44 publications

Network medicine‐based analysis of the hepatoprotective effects of Amomum villosum Lour. on alcoholic liver disease in rats

Network medicine‐based analysis of the hepatoprotective effects of Amomum villosum Lour. on alcoholic liver disease in rats

Network Medicine: A Potential Approach for Virtual Drug Screening

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