GSTM1, GSTT1, and GSTP1 Polymorphism in North Indian Population and its Influence on the Hydroquinone-Induced <i>In Vitro</i> Genotoxicity

Kumar, Munish; Chauhan, Lalit Kumar Singh; Paul, Bhola Nath; Agarwal, Sudhir K.; Goel, Sudhir K.

doi:10.1080/15376510802399057

Cited by 10 publications

(6 citation statements)

References 24 publications

(30 reference statements)

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“…Contradictory to our results, Rossi et al 23 did not find any significant association between GST genotypes ( GSTM1 and GSTT1 ) and chromosomal aberrations in exposed participants. Regarding the effect of GSTP1 polymorphism, similar to our results, Kumar et al 24 found that the null genotypes of GSTM1 , GSTT1 , and GSTP1 (val/val) are sensitive to hydroquinone genotoxicity. Individuals with the GSTP1*C (Val) allele had a higher risk of developing lung cancer than patients with wild type allele (Ryberg et al and Schneider et al).…”

Section: Discussionsupporting

confidence: 91%

Glutathione S-Transferase Gene Polymorphisms

et al. 2015

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This study investigated genetic damage in gasoline pump workers using the cytokinesis blocked micronucleus (CBMN) assay. Blood and urine samples were collected from 50 gasoline pump workers and 50 control participants matched with respect to age and other confounding factors except for exposure to benzene through gasoline vapors. To determine the benzene exposure, phenol was analyzed in urinary samples of exposed and control participants. Urinary mean phenol level was found to be significantly high (P < 0.05) in exposed workers. The CBMN frequency was found to be significantly higher in gasoline pump workers (6.70 ± 1.78) when compared to control individuals (2.20 ± 0.63; P < 0.05). We also investigated influence of polymorphisms of GSTM1, GSTT1, and GSTP1 genes on CBMN frequency. The individuals having GSTM1 and GSTT1 null genotypes had significantly higher frequency of CBMN (P < 0.05). Our study indicates that chronic and long-term exposure of gasoline vapors can increase genotoxic risk in gasoline pump workers.

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Section: Discussionsupporting

confidence: 91%

Glutathione S-Transferase Gene Polymorphisms

et al. 2015

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“…Although oxidative stress was observed (as measured by the sensitivity of yap1 Δ and the induction of the thioredoxin pathway), we found little requirement for the glutathione (glutaredoxin) pathway in yeast benezene metabolite tolerance, in contrast to data from both rodent and human epidemiological studies [15], [40], [41]. Given the contrasting data, we analyzed the growth of gsh1 Δ and glr1 Δ, involved in GSH synthesis and recycling, in the presence of HQ, CAT and BT (Figure S4).…”

Section: Resultscontrasting

confidence: 74%

Genome-Wide Functional Profiling Reveals Genes Required for Tolerance to Benzene Metabolites in Yeast

et al. 2011

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Benzene is a ubiquitous environmental contaminant and is widely used in industry. Exposure to benzene causes a number of serious health problems, including blood disorders and leukemia. Benzene undergoes complex metabolism in humans, making mechanistic determination of benzene toxicity difficult. We used a functional genomics approach to identify the genes that modulate the cellular toxicity of three of the phenolic metabolites of benzene, hydroquinone (HQ), catechol (CAT) and 1,2,4-benzenetriol (BT), in the model eukaryote Saccharomyces cerevisiae. Benzene metabolites generate oxidative and cytoskeletal stress, and tolerance requires correct regulation of iron homeostasis and the vacuolar ATPase. We have identified a conserved bZIP transcription factor, Yap3p, as important for a HQ-specific response pathway, as well as two genes that encode putative NAD(P)H:quinone oxidoreductases, PST2 and YCP4. Many of the yeast genes identified have human orthologs that may modulate human benzene toxicity in a similar manner and could play a role in benzene exposure-related disease.

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“…They did not find any association between GSTM1 and GSTT1 polymorphism and genotoxicity induced by the Benzopyrene (BaP). Similarly, no considerable relationship was found between CYP1A1, GSTM1, GSTT1, GSTP1 polymorphism and genotoxicity of trichloroethylene under both in vivo and in vitro conditions [42].…”

Section: Discussionmentioning

confidence: 90%

Effects of GST Polymorphism on Ameliorative Effect of Curcumin and Carvacrol against DNA Damage Induced by Combined Treatment of Malathion and Parathion

Kumar

Yadav

Gulati

et al. 2016

IJT

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Background: Organophosphorus pesticides has been widely used in agriculture fields to control various crop insects and their extensive use pose human life at threat because of their adverse effects on human health. In this study, we checked the effects of GST polymorphism on ameliorative effect of curcumin and carvacrol against DNA damages. Methods:Comet assay was used to assess the DNA damage and results were expressed as Tail moment. Heparinised fresh blood from healthy individuals was treated with combined concentration of malathion and parathion (i.e. 30 µg/ml of malathion and 2.5 µg/ml of parathion) in presence of combination of curcumin and carvacrol (25 µg/ml curcumin + 2.5 µg/ml carvacrol and 50 µg/ml curcumin + 5.0 µg/ml carvacrol) in order to observe the ameliorative role of curcumin and carvacrol. Multiplex PCR was performed for GSTM1 and GSTT1 genotyping.Results: Curcumin in combination with carvacrol (i.e. 25 µg/ml curcumin + 2.5 µg/ml carvacrol and 50 µg/ml curcumin + 5.0 µg/ml carvacrol) significantly reduced the DNA damage caused by combined action of malathion and parathion which supports their antigenotoxic property. No significant relationship of GSTT1 and GSTM1 polymorphism with genotoxicity of both the pesticides and antigenotoxic potential of curcumin and carvacrol was observed.Conclusion: Malathion and parathion were genotoxic in human PBL. Curcumin and carvacrol had an antigenotoxic effect against the malathion and parathion while there was not any significant effect of GSTT1 and GSTM1 polymorphism on genotoxicity of these pesticides and antigenotoxicity of curcumin and carvacrol.

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GSTM1, GSTT1, and GSTP1 Polymorphism in North Indian Population and its Influence on the Hydroquinone-Induced In Vitro Genotoxicity

Cited by 10 publications

References 24 publications

Glutathione S-Transferase Gene Polymorphisms

Glutathione S-Transferase Gene Polymorphisms

Genome-Wide Functional Profiling Reveals Genes Required for Tolerance to Benzene Metabolites in Yeast

Effects of GST Polymorphism on Ameliorative Effect of Curcumin and Carvacrol against DNA Damage Induced by Combined Treatment of Malathion and Parathion

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